Detection of a streptomycin-resistant Mycobacterium bovis strain through antitubercular drug susceptibility testing of Tunisian Mycobacterium tuberculosis complex isolates from cattle

Djemal, Saif Eddine; Camperio, Cristina; Armas, Federica; Siala, Mariam; Smaoui, Salma; Messadi-Akrout, Férièle; Gdoura, Radhouane; Marianelli, Cinzia

doi:10.1186/s12917-018-1623-9

Cited by 9 publications

(12 citation statements)

References 46 publications

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“…Comparing this large number of the recorded mutations to the number of strains in the current study indicates the large‐scale polymorphisms in M. bovis genome (Djemal et al., 2018). Many efforts have been made for the development of computational methods to predict the effects of these mutations because experimental elucidating of their effects is expensive and time‐consuming.…”

Section: Discussionsupporting

confidence: 62%

“…Bovine tuberculosis (bTB) is a chronic contagious disease caused predominantly by M . bovis and infrequently by other pathogenic mycobacteria (Djemal et al., 2018; Romha et al., 2018). The disease has serious implications on animal health and severe socio‐economic impacts due to impaired milk and meat productivity, test and slaughter regime costs, culling of infected animals, carcass condemnation, movement controls and international trade restrictions (Vordermeier et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

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Abattoir survey of bovine tuberculosis in tanta, centre of the Nile delta, with in silico analysis of gene mutations and protein–protein interactions of the involved mycobacteria

Borham

Oreiby

El-Gedawy³

et al. 2021

Transbounding Emerging Dis

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Bovine tuberculosis is a transboundary disease of high economic and public health burden worldwide. In this study, post‐mortem examination of 750 cattle and buffalo in Tanta abattoir, Centre of the Nile Delta, revealed visible TB in 4% of animals and a true prevalence of 6.85% (95% CI: 5.3%–8.9%). Mycobacterial culture, histopathology and RT‐PCR targeting all members of M. tuberculosis complex were performed, upon which 85%, 80% and 100% of each tested lesions were confirmed as TB, respectively. Mpb70‐targeting PCR was conducted on ten RT‐PCR positive samples for sequencing and identified nine Mycobacterium (M.) bovis strains and, interestingly, one M. tuberculosis (Mtb) strain from a buffalo. Bioinformatics tools were used for prediction of mutations, nucleotide polymorphisms, lineages, drug resistance and protein–protein interactions (PPI) of the sequenced strains. The Mtb strain was resistant to rifampicin, isoniazid and streptomycin, and to the best of our knowledge, this is the first report of multidrug resistant (MDR)‐Mtb originating from buffaloes. Seven M. bovis strains were resistant to ethambutol and ethionamide. Such resistances were associated with KatG, rpoB, rpsL, embB and ethA genes mutations. Other mutations and nucleotide polymorphisms were also predicted, some are reported for the first time and require experimental work for validation. PPI revealed more interactions than what would be expected for a random set of proteins of similar size and had dense interactions between nodes that are biologically connected, as a group. Two M. bovis strains belonged to BOV AFRI lineage (Spoligotypes BOV 1; BOV 2) and eight strains belonged to East‐Asian (Beijing) lineage. In conclusion, visible TB was prevalent in the study area, RT‐PCR is the best to confirm the disease, MDR‐Mtb is associated with buffalo TB, and mycobacteria of different lineages carry many resistance genes to chemotherapeutic agents used in treatment of human TB constituting a major public health risk.

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Section: Discussionsupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Abattoir survey of bovine tuberculosis in tanta, centre of the Nile delta, with in silico analysis of gene mutations and protein–protein interactions of the involved mycobacteria

Borham

Oreiby

El-Gedawy³

et al. 2021

Transbounding Emerging Dis

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“…At present, mutation of the rst-line anti-tuberculosis drug resistance genes have been extensively studied at home and abroad, such as INH, RFP, EMB and SM. Studies showed that the mutation sites of the drug resistance genes of the above anti-tuberculosis drugs are quite different in different regions [21] .This study showed that the mutation rates of isoniazid resistance gene katG, rifampicin resistance gene rpoB, ethambutol resistance gene embB, streptomycin resistance gene rpsL and RRs in multidrug resistance group were higher than those in full-sensitivity group, which consistent with the results of domestic or foreign research [22][23][24][25] .However, there was no mutation in inhA gene of isoniazid-resistant group and full-sensitive group, which was inconsistent with the results of Asho Ali et al The results showed that the mutation rate of inhA gene was about 8% [26] . This difference may relate with geographical differences.…”

Section: Discussionsupporting

confidence: 86%

Gene Mutation Characteristics and Cluster Analysis of Multidrug-Resistant Mycobacterium Tuberculosis in Urumqi

Yang

Yadikaer²,

Chen

et al. 2021

Preprint

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Objective To understand the molecular biological characteristics of multidrug-resistant Mycobacterium tuberculosis in Urumqi by the analysis of gene mutation and clustering of multidrug-resistant Mycobacterium tuberculosis in Urumqi, to provid theoretical basis for the prevention and control of multidrug-resistant tuberculosis in the future. Methods The tuberculosis patients who were diagnosed, registered, cultured positive Mycobacterium tuberculosis and over 16 years old in Urumqi were studied. Geno Type MTBDR plus Assay and MIRU-VNTR were used to analysis the gene mutation and clustering of multidrug-resistant mycobacterium tuberculosis in Urumqi. Result Mutation gene included Isoniazid resistance gene katG 315; Rifampicin resistance gene rpoB 526, 531; Ethambutol resistance gene embB 206; Streptomycin resistance gene rpsL 43 and RRs 1401; Ofloxacin resistance gene gyrA 74, 90, 91 and 94. It was shown that the Beijing genotype was predominant (49.530%) among these319strains. There were 14 strains multi-drug resistant strains, it was predominant (73.684%) of multi-drug resistant strains. Multi-drug resistant prevalence of Beijing strains was 8.861%. The clustering rate of MDR-TB was 5.263% in Urumqi. Conclusion Molecular biological characteristics of multidrug-resistant Mycobacterium tuberculosis have some particularities in Urumqi. Therefore, modern molecular epidemiological techniques such as molecular biology and DNA fingerprinting can be used to improve the speed of diagnosis and to carry out case tracking and source investigation.

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“…M. bovis exhibits an intrinsic resistance to pyrazinamide, a first-line anti-TB agent that is included in the standard treatment regimen offered to new TB patients [ 8 – 10 ]. Furthermore, reports of primary resistance to isoniazid, rifampicin and streptomycin primary resistance have recently emerged [ 3 , 8 ]. Multidrug resistant (MDR) forms of M. bovis have also been isolated [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

A case report of transmission and disease caused by Mycobacterium caprae and Mycobacterium bovis in Lima, Peru

Shrestha

Picoy²,

Torres

et al. 2021

BMC Infect Dis

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Background The Tuberculosis (TB) burden in Peru is significant with respect to both disease morbidity and mortality. Furthermore the recent diversification of farming enterprise to include a wide range of animal species has necessitated the consideration of members of the Mycobacterium Tuberculosis Complex (MTBC) with the potential for zoonotic transmission. M. bovis and M. caprae, a lesser known member of the MTBC exhibit an exceptionally wide host spectrum in animals and are capable of causing disease in humans. M. bovis has a predictable resistance profile which includes resistance to pyrazinamide. Thus, failure to identify M. bovis as the causative agent in reported TB cases leads to higher levels of treatment failure and contributes to the transmission of drug-resistant TB. Case presentation Reported here are the clinical presentations, investigations and treatment histories of two patients identified from a population level genotyping study in Lima, Peru that were at the time of treatment thought to be M. tuberculosis patients but in retrospect were spectated using whole genome sequencing as M. caprae and M. Bovis. Conclusions The cases reported here constitute convincing evidence that M. caprae and M. bovis are causative agents of TB infection in humans in Peru and underscore the importance of species-level MTBC member identification to effectively control and treat zoonotic TB. Furthermore these cases highlight the challenges of using clinical risk factors to identify cases of zoonotic TB in humans as their clinical presentation and transmission history is often difficult to distinguish from anthroponotic TB.

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Detection of a streptomycin-resistant Mycobacterium bovis strain through antitubercular drug susceptibility testing of Tunisian Mycobacterium tuberculosis complex isolates from cattle

Cited by 9 publications

References 46 publications

Abattoir survey of bovine tuberculosis in tanta, centre of the Nile delta, with in silico analysis of gene mutations and protein–protein interactions of the involved mycobacteria

Abattoir survey of bovine tuberculosis in tanta, centre of the Nile delta, with in silico analysis of gene mutations and protein–protein interactions of the involved mycobacteria

Gene Mutation Characteristics and Cluster Analysis of Multidrug-Resistant Mycobacterium Tuberculosis in Urumqi

A case report of transmission and disease caused by Mycobacterium caprae and Mycobacterium bovis in Lima, Peru

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