Detection of a Toxic Phenolic Compound in Cottonseed Extract and its Products

Mannan, S. Mudi; Shad, Muhammad Aslam; Perveen, Asia

doi:10.3923/pjn.2010.994.997

Cited by 5 publications

(2 citation statements)

References 9 publications

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“…Formic acid and acetic acid in the SF5 were analyzed by a Dionex ion chromatography ICS 5000 system (Sunnyvale, CA) with a Dionex IonPac ® ICE‐AS1 column (4 × 250 mm 2 ) . The concentration of total phenolic compounds was measured by a colorimetric method as described previously . As one of the most important phenols identified by GC/MS, vanillin was chosen as the calibration standard.…”

Section: Methodsmentioning

confidence: 99%

Microalgae fermentation of acetic acid‐rich pyrolytic bio‐oil: Reducing bio‐oil toxicity by alkali treatment

Zhao¹,

Chi

Rover

et al. 2013

Env Prog and Sustain Energy

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Bio‐oil derived from fast pyrolysis of lignocellulosic biomass contains various substrates that can be fermented to fuels and chemicals. The goal of this research was to utilize an acetic acid‐rich fraction of bio‐oil for the growth and lipid production by microalga Chlamydomonas reinhardtii. As toxic compounds are contained in the bio‐oil, the algae cannot survive in medium containing this bio‐oil fraction even at a low level (0.05 wt %). An alkali‐based treatment with sodium hydroxide was used to reduce the toxicity and enhance its fermentability by microalgae. It was found that treating the acetic acid‐rich bio‐oil fraction by adjusting pH to 10 greatly improved the algal growth. The algae can thrive in medium containing 4 wt % alkali‐treated bio‐oil fraction. When using a metabolic‐evolved strain with high level of toxicity tolerance, the algae were even capable of growing in medium containing 5.5 wt % bio‐oil fraction, which replaced 100% of the acetic acid in the medium. The algal biomass grown in medium containing alkali‐treated bio‐oil fraction exhibited fatty acid profiles similar to the culture grown in pure acetic acid, but with a lower total fatty acid content. but the total fatty acid content (∼10% DW) was lower than that of the control (∼20% DW).The benefit of alkali treatment for enhancing algal growth was confirmed to be due to the removal of toxic compounds such as furfural, acetol, phenolics, and 5‐hydroxymethylfurfural (HMF). Collectively, the results showed that fast pyrolysis‐microalgal fermentation is a viable approach for producing lipid from lignocellulosic biomass. Alkali‐based treatment is an effective method for reducing bio‐oil toxicity, and thereby, greatly enhancing the algae fermentability of bio‐oil. © 2013 American Institute of Chemical Engineers Environ Prog, 32: 955–961, 2013

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Section: Methodsmentioning

confidence: 99%

Microalgae fermentation of acetic acid‐rich pyrolytic bio‐oil: Reducing bio‐oil toxicity by alkali treatment

Zhao¹,

Chi

Rover

et al. 2013

Env Prog and Sustain Energy

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“…Studies conducted at different thermodynamic conditions have revealed that gossypol has a very high boiling point of 177-183°C, and heating of cottonseeds during oil extraction binds gossypol to proteins thus reducing protein availability in cottonseed meals [11]. Excessive consumption of cotton seeds by animals has been known to result in gossypol toxicosis.…”

Section: Introductionmentioning

confidence: 99%

Comparative profiling of gossypol enantiomers and seed traits’ related transcripts in cotton lines

Felts

Bhatta²,

Bhatti³

et al. 2019

PAB

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Cotton (Gossypium spp.) is the world's third most important oilseed crop as well as an economic source of protein. However, the presence of (-)-gossypol limits the nutritional value of cottonseeds. To genetically profile relevant traits, seeds of the two cotton parental lines TM-1 (G. hirstum) and 3-79 (G. barbadense) along with their 17 chromosomal substitution (CS) progenies were quantified for (-)-and (+)-enantiomer levels. The TM-1 parent was found to contain a higher ratio of (+)-:(-)-gossypol enantiomers (13.34:8.52 µg/mg) compared to that of 3-79 levels (6.69:7.41 µg/mg) in the seeds. Among progeny CS-B (containing substituted chromosomes from G. barbadense in G. hirsutum background) lines, only CS-B17 was found to contain significantly lower (7.65 µg/mg) level of (+)-enantiomer in its seeds, and also had the lowest (5.09 µg/mg) amount of (-)-gossypol among all lines. A singular protocol was then devised for RNA yields from polysaccharide-rich cottonseeds of CS-B17 and its parents. Transcription derived fragments (TDFs) analyses of these three lines were conducted on seeds harvested at 28, 35 and 42 days post anthesis (DPA). 64 TDFs profiles were analyzed using differential expression analyses, and similarities were revealed between 3-79 and CS-B17 along with unique expression trends for CS-B17. Such expression analyses, which are based on single chromosome differences with TM-1 parent while reflecting seed nutritional merit of 3-79 parent, may facilitate cotton breeding endeavors.

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Green tea catechins suppress xanthine oxidase activity in dairy products: An improved HPLC analysis

Rashidinejad

Birch

Everett

2016

Journal of Food Composition and Analysis

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Detection of a Toxic Phenolic Compound in Cottonseed Extract and its Products

Cited by 5 publications

References 9 publications

Microalgae fermentation of acetic acid‐rich pyrolytic bio‐oil: Reducing bio‐oil toxicity by alkali treatment

Microalgae fermentation of acetic acid‐rich pyrolytic bio‐oil: Reducing bio‐oil toxicity by alkali treatment

Comparative profiling of gossypol enantiomers and seed traits’ related transcripts in cotton lines

Green tea catechins suppress xanthine oxidase activity in dairy products: An improved HPLC analysis

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