Detection of a Traumatic Brain Injury Biomarker at the 10 fg/mL Level

Mathew, Anup; Shi, Xuyang; Yau, S.‐T.

doi:10.1007/s40291-018-0365-5

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…Even though other studies report a wider range of detection and lower LODs for S100B [ 40 – 42 ], this work demonstrates an effective detection of S100B in a clinically relevant range since plasmatic S100B concentrations lower than a cutoff of 100pg/mL [ 9 ] ruled out the presence of bleeding in the CT, while levels below 30pg/mL ruled out BBB disruption [ 10 ]. Furthermore, many of the sensors found in the literature exhibit limitations for a fast analysis due to the requirement of sandwich-type immunoassays and fluorescent labels.…”

Section: Discussionmentioning

confidence: 75%

TBISTAT: An open-source, wireless portable, electrochemical impedance spectroscopy capable potentiostat for the point-of-care detection of S100B in plasma samples

et al. 2022

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Point-of-Care (POC) testing for biomarker detection demands techniques that are easy to use, readily available, low-cost, and with rapid response times. This paper describes the development of a fully open-source, modular, wireless, battery-powered, smartphone-controlled, low-cost potentiostat capable of conducting electrochemical impedance spectroscopy for the electrochemical detection of the S100B protein captured in an ANTI-S100B functionalized thin-film gold interdigitated electrode platform to support traumatic brain injury diagnosis and treatment. EIS results from the developed potentiostat were validated with a commercial benchtop potentiostat by comparing impedance magnitude and phase values along the EIS frequency range. In addition, an experimental design was performed for detecting S100B in spiked human plasma samples with S100B concentrations of clinical utility, and a calibration curve was found for quantifying S100B detection. No statistically significant differences were found between EIS results from the developed potentiostat and the commercial potentiostat. Statistically significant differences in the changes in charge transfer resistance signal between each tested S100B concentration (p < 0.05) were found, with a limit of detection of 35.73 pg/mL. The modularity of the proposed potentiostat allows easier component changes according to the application demands in power, frequency excitation ranges, wireless communication protocol, signal amplification and transduction, precision, and sampling frequency of ADC, among others, when compared to state-of-the-art open-source EIS potentiostats. In addition, the use of minimal, easy acquirable open-source hardware and software, high-level filtering, accurate ADC, Fast Fourier Transform with low spectral leakage, wireless communication, and the simple user interface provides a framework for facilitating EIS analysis and developing new affordable instrumentation for POC biosensors integrated systems.

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Section: Discussionmentioning

confidence: 75%

TBISTAT: An open-source, wireless portable, electrochemical impedance spectroscopy capable potentiostat for the point-of-care detection of S100B in plasma samples

et al. 2022

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“…To achieve voltage-controlled amplification, the WE has been connected to the gating electrode via a DC power supply to apply the gating voltage ( V G ) yielding an electric field at the WE/solution interface and thus resulting in changes of interfacial charge distribution. 130 The proposed technique enabled LDL as low as 10 fg mL −1 in serum which is sufficient for S100β quantification (cutoff for mTBI 42 pg mL −1 , 37 Table 1 ). 130 Importantly, this is one of very few publications on FED-based architectures reporting its successful application in undiluted serum.…”

Section: Types Of Electrochemical (Bio)sensors For Detection Of Protein Biomarkers Relevant To Mtbimentioning

confidence: 97%

“… 130 The proposed technique enabled LDL as low as 10 fg mL −1 in serum which is sufficient for S100β quantification (cutoff for mTBI 42 pg mL −1 , 37 Table 1 ). 130 Importantly, this is one of very few publications on FED-based architectures reporting its successful application in undiluted serum.…”

Section: Types Of Electrochemical (Bio)sensors For Detection Of Protein Biomarkers Relevant To Mtbimentioning

confidence: 97%

“…The key issue limiting SAM practical applications remains however the overall stability of the monolayer lm. 126,127 CNTs have also found their application for transducer modication in various composites, 128,129 such as AuPd-multiwall carbon nanotubes (MWCNTs) for the detection of the NSE biomarker, 128 CNTs with Naon and glutaraldehyde for the detection of S100b, 130 MWCNTs incorporated into molecularly imprinted polymer (MIP) layer for the detection of GFAP 131 and MWCNTs with reduced graphene oxide (rGRO) and chitosan for detection of tau-441 protein. 132 Nanostructured conducting polymers (CPs) such as PPy-NWs functionalized via diazonium coupling reaction 113 or (nano)composite layer (e.g.…”

Section: Design Of Electrochemical Sensors For Detection Of Protein Biomarkers Relevant To Mtbimentioning

confidence: 99%

“…A peculiar strategy based on field-effect enzymatic detection (FEED) reported by Mathew et al has adopted gating voltage for signal amplification for ultrasensitive detection of S100β. 130 In this approach (see Fig. 7 ), a sandwich assay with an HRP-labelled detection antibody has been realized on a working electrode (WE) using a conventional screen-printed three-electrode cell.…”

Section: Types Of Electrochemical (Bio)sensors For Detection Of Protein Biomarkers Relevant To Mtbimentioning

confidence: 99%

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Electrochemical sensing of blood proteins for mild traumatic brain injury (mTBI) diagnostics and prognostics: towards a point-of-care application

2021

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In Vitro Detection of S100B and Severity Evaluation of Traumatic Brain Injury Based on Biomimetic Peptide‐Modified Nanochannels

Zhang,

Wang

et al. 2023

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The diagnosis and evaluation of traumatic brain injury (TBI) are crucial steps toward the treatment and prognosis of patients. A common question remains as to whether it is possible to introduce an ideal device for signal detection and evaluation that can directly connect digital signals with TBI, thereby enabling prompt response of the evaluation signal and sensitive and specific functioning of the detection process. Herein, a method is presented utilizing polymetric porous membranes with TRTK‐12 peptide‐modified nanochannels for the detection of S100B (a TBI biomarker) and assessment of TBI severity. The method leverages the specific bonding force between TRTK‐12 peptide and S100B protein, along with the nanoconfinement effect of nanochannels, to achieve high sensitivity (LOD: 0.002 ng mL−1) and specificity (∆I/I0: 44.7%), utilizing ionic current change as an indicator. The proposed method, which is both sensitive and specific, offers a simple yet responsive approach for real‐time evaluation of TBI severity. This innovative technique provides valuable scientific insights into the advancement of future diagnostic and therapeutic integration devices.

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Detection of a Traumatic Brain Injury Biomarker at the 10 fg/mL Level

Cited by 7 publications

References 16 publications

TBISTAT: An open-source, wireless portable, electrochemical impedance spectroscopy capable potentiostat for the point-of-care detection of S100B in plasma samples

TBISTAT: An open-source, wireless portable, electrochemical impedance spectroscopy capable potentiostat for the point-of-care detection of S100B in plasma samples

Electrochemical sensing of blood proteins for mild traumatic brain injury (mTBI) diagnostics and prognostics: towards a point-of-care application

In Vitro Detection of S100B and Severity Evaluation of Traumatic Brain Injury Based on Biomimetic Peptide‐Modified Nanochannels

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