Detection of accessory gene regulator groups genes and cassette chromosome mec types among Staphylococcus aureus isolated from intensive care unit patients

Ghasemian, Abdolmajid; Peerayeh, Shahin Najar; Bakhshi, Bita; Mirzaee, Mohsen

doi:10.1016/s2222-1808(14)60643-5

Cited by 23 publications

(17 citation statements)

References 27 publications

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“…The majority of MRSA strains in this study harbored SCCmec type III, which is important in healthcare associated infections or HA-MRSA infections. Similarly, our previous studies and Japoni's survey from south of Iran, depicted that SCCmec type III was the predominant SCCmec type (19)(20)(21). In this study, one MRSA with SCCmec type III was resistant to all the antibiotics, except for vancomycin and linezolid.…”

Section: Discussionsupporting

confidence: 67%

The Comparison ofStaphylococcus aureusIsolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

Ghasemian¹,

Peerayeh²,

Bakhshi³

2015

Avicenna J Clin Microbiol Infect

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Background:The polysaccharide intercellular adhesion (PIA) produced by Staphylococcus aureus is effective in the protection of isolates from outer harsh conditions and progress of infection. Objectives: The aim of this study was to compare the icaABCD genes encoding polysaccharide intercellular adhesion (PIA) between blood and wound isolates of Staphylococcus aureus (S. aureus) in Tehran. Patients and Methods: Forty-eight clinical isolates (including 30 blood and 18 skin wounds) were collected from patients and were identified. Next, mecA gene, SCCmec types and icaABCD genes were detected among blood and wound isolates of S. aureus by PCR assay and specific primers. Results: Nine (19%) out of 12 methicillin resistant S. aureus (MRSA) isolates harbored SCCmec type III and three (6.2%) isolates harbored SCCmec type V. Prevalence of icaA, icaB, icaC and icaD in blood isolates was twenty-one (70%), fourteen (48%), nineteen (64%) and eighteen (60%), respectively; while the prevalence in wound isolates was as nine (50%), seven (39%), six (34%) and twelve (67%), respectively. Conclusions: These findings showed no significant difference regarding the presence of icaADBC genes between blood and wound isolates.

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Section: Discussionsupporting

confidence: 67%

The Comparison ofStaphylococcus aureusIsolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

Ghasemian¹,

Peerayeh²,

Bakhshi³

2015

Avicenna J Clin Microbiol Infect

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“…In Press(In Press):e14495. (39). The frequency of toxin and adhesive moleculeencoding genes in isolates with agr type I was found to be higher than that for type III in the present study, which is in line with the results reported by other studies in various areas (40).…”

Section: U N C O R R E C T E D P R O O Fsupporting

confidence: 93%

Molecular Investigation of Methicillin-Resistant Staphylococcus aureus Strains Recovered from the Intensive Care Unit (ICU) Based on Toxin, Adhesion Genes and agr Locus Type Analysis

Nasirian

Saadatmand

Goudarzi

et al. 2018

Arch Clin Infect Dis

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Background: Staphylococcus aureus, as one of the most common causes of nosocomial infections, has widely spread to all parts of the world and is becoming a serious concern in public health. Objectives: The present study aimed at evaluating the prevalence of adhesion and toxin gene profiles and their distribution among different agr types. Methods: The current cross-sectional study was performed in Tehran, Iran, by analyzing 125 methicillin resistant Staphylococcus aureus (MRSA) strains isolated from hospitalized patients at the ICUs from March, 2016 to January, 2017. In vitro antibiotic susceptibility testing of isolates was assessed using the Kirby-Bauer disk diffusion method. The MRSA strains were genetically typed by agr typing and virulence and adhesion genes profile by conventional PCR. Results: Antibiotic susceptibility testing showed that inducible macrolide-lincosamide-streptogramin B, constitutive macrolidelincosamide-streptogramin B, and high-level mupirocin resistance phenotypes had a frequency of 18 (14.4%), 50 (56%), and 10 (31.3%), respectively. The predominant resistance profile among MDR-MRSA isolates included resistance profile to seven antibiotics (32%). A total of ten virulence genotypes were observed, from which genotype spa, clfA, clfB, fnbB, fnbA, ebp, and can / tst (36%, 45/125) comprised the majority followed by spa, clfA, clfB, and fnbB (24%, 30/125). Type I was the most prevalent agr type (52%), followed by type III (34.4%), type II (9.6%), I 5(5.3%), and IV (4%). All isolates carrying PVL-encoding genes and HLMUPR-MRSA strains corresponded exclusively to agr type I. Conclusions:The current data demonstrated that virulence gene profiles among different agr types of MRSA isolates were divers. The present study suggests that molecular characterization of MRSA strains should periodically be studied.

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“…Drug resistance is increasingly developing amongst nosocomial pathogens (13,14). Approximately 0.3% of P. aeruginosa genes encode agents for antibiotic resistance (15).…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of blaVEB-1 Beta-Lactamase Encoding Gene Among Extended Spectrum B-Lactamase Positive Wound Isolates of Pseudomonas aeruginosa

Davodian

Sadeghifard

Ghasemian

et al. 2015

Arch Pediatr Infect Dis

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Background: Pseudomonas aeruginosa is considered as a leading cause of nosocomial infections. Burn and wound infections are mainly caused by multidrug-resistant P. aeruginosa isolates. Drug resistance frequently occurs among nosocomial isolates and can usually resist a myriad of antibiotics such as novel β-lactam antibiotics. Detection of multidrug-resistant isolates could assist better drug administration. Objectives: The aim of this study was to detect Extended Spectrum Beta-Lactamases (ESBL) positive wound isolates and the genes encoding bla VEB-1 ESBL among wound isolates of P. aeruginosa. Materials and Methods: A total of 89 wound isolates of P. aeruginosa were collected from patients (47% (n = 42) were male and 53% (n = 47) were female) at six Iranian hospitals between years 2009 and 2011. Antibiotic susceptibility and phenotypic ESBL production tests were conducted. The combined disk was used to determine ESBLs production. The bla VEB-1 gene was detected with the polymerase chain reaction (PCR). Results: The majority of the wound isolates were resistant to augmentin (90%, n = 80) and cefpodoxime (87.6%, n = 78). However, the majority was susceptible to imipenem and meropenem. Fifty-eight (42%) wound isolates were ESBL positive. The antibiotic resistance amongst ESBL positive isolates was relatively higher than ESBL negative isolates. Twenty-three (40%) ESBL-positive isolates amplified the bla VEB-1 gene. Conclusions: More than behalf of the wound isolates were ESBL positive, and the presence of bla VEB-1 was determined in less than half of these isolates. Fortunately, resistance to imipenem and meropenem was low.

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Detection of accessory gene regulator groups genes and cassette chromosome mec types among Staphylococcus aureus isolated from intensive care unit patients

Cited by 23 publications

References 27 publications

The Comparison ofStaphylococcus aureusIsolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

The Comparison ofStaphylococcus aureusIsolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

Molecular Investigation of Methicillin-Resistant Staphylococcus aureus Strains Recovered from the Intensive Care Unit (ICU) Based on Toxin, Adhesion Genes and agr Locus Type Analysis

Molecular Detection of blaVEB-1 Beta-Lactamase Encoding Gene Among Extended Spectrum B-Lactamase Positive Wound Isolates of Pseudomonas aeruginosa

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