Detection of Aichi virus genotype B in two lines of wastewater treatment processes

Ibrahim, Chourouk; Hammami, Salah; Mejri, Selma; Mehri, Inès; Pothier, Pierre; Hassen, Abdennaceur

doi:10.1016/j.micpath.2017.06.001

Cited by 25 publications

(20 citation statements)

References 39 publications

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“…Automatic extractors have been used by Ibrahim et al (2017) and Di Bonito et al (2017) to extract nucleic acids of viruses from influent and effluent wastewater samples. Most of the automated extractors rely on the use of magnetic beads which bind the nucleic acids, leaving impurities in the solution.…”

Section: Methods Of Pre-treatment Of Wastewater Samplesmentioning

confidence: 99%

“…There have been numerous studies on the detection of viruses in different water matrices, including: surface water treated for drinking water purposes ( Wang et al, 2020b ), bottled drinking water ( Martin-Latil et al, 2012 ), well water ( Emelko et al, 2019 ; Ji et al, 2020 ), seawater ( Canh et al, 2019 ), irrigation water ( Rusiñol et al, 2020 ), surface water ( Canh et al, 2019 ; Guo et al, 2018 ; Hata et al, 2014 ; Khalenkov et al, 2008 ) and wastewater ( Di Bonito et al, 2017 ; Ibrahim et al, 2017 ; Jahne et al, 2020 ; Masclaux et al, 2013 ; Prado et al, 2019 ; Qiu et al, 2016 ; Symonds et al, 2014 ).…”

Section: Detection Methods Of Viruses In Wastewatermentioning

confidence: 99%

“…Samples were taken from influents, primary settling tanks, secondary and tertiary treatment effluents, and final effluents. Some of these studies examined the fate of viruses inside WWTPs by examining samples originating from secondary treatment steps ( Ibrahim et al, 2017 ; Prado et al, 2011 , Prado et al, 2019 ; Symonds et al, 2014 ). Other studies collected samples prior to and after tertiary treatment to determine its effectiveness in the context of the removal of viruses ( Prado et al, 2011 ; Qiu et al, 2018 ).…”

Section: Detection Methods Of Viruses In Wastewatermentioning

confidence: 99%

“…As discussed, the sample processing methods affect the efficiency of subsequent detection methods. The choice of a specific method for detection or quantification also depends on the ease of cultivation of the viruses in laboratory conditions ( Ibrahim et al, 2017 ). Specific characteristics of the viruses affect the concentration and detection methods to be applied to the samples being examined.…”

Section: Detection Methods Of Viruses In Wastewatermentioning

confidence: 99%

“… Ibrahim et al (2017) utilized the RT-PCR technique to amplify nucleic acids of AiVs, which have RNA genomes, in wastewater samples. Amplification with RT-PCR enabled the analysis of AiVs which are not easily cultivable in the laboratory.…”

Section: Detection Methods Of Viruses In Wastewatermentioning

confidence: 99%

See 4 more Smart Citations

Viruses in wastewater: occurrence, abundance and detection methods

Corpuz

Buonerba

Vigliottá

et al. 2020

Science of The Total Environment

228

178

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This paper presents an updated and comprehensive review on the different methods used for detection and quantification of viruses in wastewater treatment systems. The analysis of viability of viruses in wastewater and sludge is another thrust of this review. Recent studies have mostly focused on determining the abundance and diversity of viruses in wastewater influents, in samples from primary, secondary, and tertiary treatment stages, and in final effluents. A few studies have also examined the occurrence and diversity of viruses in raw and digested sludge samples. Recent efforts to improve efficiency of virus detection and quantification methods in the complex wastewater and sludge matrices are highlighted in this review. A summary and a detailed comparison of the pre-treatment methods that have been utilized for wastewater and sludge samples are also presented. The role of metagenomics or sequencing analysis in monitoring wastewater systems to predict disease outbreaks, to conduct public health surveillance, to assess the efficiency of existing treatment systems in virus removal, and to re-evaluate current regulations regarding pathogenic viruses in wastewater is discussed in this paper. Challenges and future perspectives in the detection of viruses, including emerging and newly emerged viruses such as the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), in wastewater systems are discussed in this review.

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Section: Methods Of Pre-treatment Of Wastewater Samplesmentioning

confidence: 99%

Section: Detection Methods Of Viruses In Wastewatermentioning

confidence: 99%

Section: Detection Methods Of Viruses In Wastewatermentioning

confidence: 99%

Section: Detection Methods Of Viruses In Wastewatermentioning

confidence: 99%

Section: Detection Methods Of Viruses In Wastewatermentioning

confidence: 99%

See 3 more Smart Citations

Viruses in wastewater: occurrence, abundance and detection methods

Corpuz

Buonerba

Vigliottá

et al. 2020

Science of The Total Environment

228

178

View full text Add to dashboard Cite

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Detection of Hepatovirus a in Two Tunisian Wastewater Treatment Plants

Ibrahim

Hammami

Hassen

2021

Recent Advances in Environmental Science From the Euro-Mediterranean and Surrounding Regions (2nd Edition)

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Molecular detection and genotypic characterization of enteric adenoviruses in a hospital wastewater

Ibrahim

Hassen

Pothier³

et al. 2018

Environ Sci Pollut Res

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Hospital wastewater (HWW) represents a major source of the diffusion of many antibiotics and some toxic pathogenic microorganisms in the aquatic environment. Sanitation services play a critical role in controlling transmission of numerous waterborne pathogens, especially enteric human adenoviruses (HAdVs) that can cause acute gastroenteritis. This study intended to evaluate the human adenoviruses (HAdVs) detection rates, to determine the genotype of these viruses and to assess the efficiency of HAdVs removal in hospital pilot wastewater treatment plant (PWWTP) in Tunis City, Tunisia. Therefore, hospital wastewater samples (n = 102) were collected during the study year from the two biological wastewater treatment techniques: natural oxidizing ponds and the rotating biological disks or biodisks. Nested polymerase chain reaction (Nested PCR) was used to evaluate the HAdVs detection rates. The genotype of HAdVs positive samples was achieved by the sequencing of the PCR products. HAdVs were detected in 64% (65/102) of positive wastewater samples. A substantial increase in the frequencies of HAdVs was observed at the exit of the two wastewater treatment techniques studied. The typing of HAdVs species F showed the occurrence of only HAdVs type 41. This data acquired for the first time in Tunisia showed high persistence and survival of HAdVs in the two biological wastewater treatment techniques experienced, and mainly highlighted the poor virological quality of the treated wastewater intended for recycling, agriculture reuse, and discharges into the natural receiving environments. Consequently, tertiary wastewater treatment appeared necessary in this case to decrease the load of enteric viruses flowing in the water environment.

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Detection of Aichi virus genotype B in two lines of wastewater treatment processes

Cited by 25 publications

References 39 publications

Viruses in wastewater: occurrence, abundance and detection methods

Viruses in wastewater: occurrence, abundance and detection methods

Detection of Hepatovirus a in Two Tunisian Wastewater Treatment Plants

Molecular detection and genotypic characterization of enteric adenoviruses in a hospital wastewater

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