“…Airborne dust was sampled at one site each from 13 Holstein dairy barns (eastern Quebec, Canada). Three different air samplers were used, namely, the Institute of Occupational Medicine cassettes (SKC, Ancaster, ON, Canada) or IOM samplers, loaded with 25-mm-diameter gelatin membranes (SKC) and plugged into a Giliar-5 pump (Levitt-Sécurité Limitée, Dorval, QC, Canada), at 2 liters/min (50% cutoff size of 4.0 m); the Coriolis (Bertin Technologies, Montigny-le-Bretonneux, France), which collects 100% of the particles of 4.4 m, at 100 liters/min and loaded with 15 ml of 0.9% saline solution; and the NIOSH two-stage bioaerosol cyclone (BC 251) sampler (31,42), plugged into an AirCon-2 pump (Gilian) and sampling at 10 liters/min for particulate size separation. The size distribution for each stage (50% cutoff size) of the NIOSH sampler was as follows: 2.1 m for the first stage and 0.41 m for the second stage.…”