Detection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis

Lu, Chong; Hou, Xianliang; Li, Minwei; Wang, Lin; Zeng, Ping; Jia, Hongyu; Chen, Jianing; Wei, Yu; He, Hong; Liu, Xiangdong; Diao, Hongyan

doi:10.1038/s41598-017-00906-1

Cited by 15 publications

(19 citation statements)

References 43 publications

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“…A previous study reported that IL-6 and IFN-γ levels were significantly increased in the saliva of PBC patients. Moreover, the IL-6 and IFN-γ levels in the saliva of PBC patients are positively associated with those in the sera of those patients [ 41 ]. Similarly, elevated levels of salivary IL-1β, IL-6, and secretory IgA in cirrhosis patients have also been reported [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Dysbiosis of oral microbiota and its association with salivary immunological biomarkers in autoimmune liver disease

et al. 2018

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The gut microbiota has recently been recognized to play a role in the pathogenesis of autoimmune liver disease (AILD), mainly primary biliary cholangitis (PBC) and autoimmune hepatitis (AIH). This study aimed to analyze and compare the composition of the oral microbiota of 56 patients with AILD and 15 healthy controls (HCs) and to evaluate its association with salivary immunological biomarkers and gut microbiota. The subjects included 39 patients with PBC and 17 patients with AIH diagnosed at our hospital. The control population comprised 15 matched HCs. Salivary and fecal samples were collected for analysis of the microbiome by terminal restriction fragment length polymorphism of 16S rDNA. Correlations between immunological biomarkers measured by Bio-Plex assay (Bio-Rad) and the oral microbiomes of patients with PBC and AIH were assessed. Patients with AIH showed a significant increase in Veillonella with a concurrent decrease in Streptococcus in the oral microbiota compared with the HCs. Patients with PBC showed significant increases in Eubacterium and Veillonella and a significant decrease in Fusobacterium in the oral microbiota compared with the HCs. Immunological biomarker analysis showed elevated levels of inflammatory cytokines (IL-1β, IFN-γ, TNF-α, IL-8) and immunoglobulin A in the saliva of patients with AILD. The relative abundance of Veillonella was positively correlated with the levels of IL-1β, IL-8 and immunoglobulin A in saliva and the relative abundance of Lactobacillales in feces. Dysbiosis of the oral microbiota is associated with inflammatory responses and reflects changes in the gut microbiota of patients with AILD. Dysbiosis may play an important role in the pathogenesis of AILD.

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Section: Discussionmentioning

confidence: 99%

Dysbiosis of oral microbiota and its association with salivary immunological biomarkers in autoimmune liver disease

et al. 2018

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“…CSPs are considered as carriers of odorants in insect chemoreception 2. They are typically found in various insect tissues and perform divergent functions 42. Here, we cloned MsepCSP5 from M. separata which shares high sequence similarity with other CSPs and possesses four conserved cysteine connected by two disulfide bonds between neighbor residues showing the typical characteristics of CSPs 43.…”

Section: Discussionmentioning

confidence: 99%

A chemosensory protein MsepCSP5 involved in chemoreception of oriental armyworm Mythimna separata

et al. 2018

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Chemosensory proteins (CSPs) have been suggested to perform several functions in insects, including chemoreception. To find out whether MsepCSP5 identified from Mythimna separata shows potential physiological functions in olfaction, gene expression profiles, ligand-binding experiments, molecular docking, RNA interference, and behavioral test were performed. Results showed that MsepCSP5 was highly expressed in female antennae. MsepCSP5 showed high binding affinities to a wide range of host-related semiochemicals, and displayed that 26 out of 35 candidate volatiles were highly bound (Ki < 10 µM) at pH 5.0 rather than pH 7.4. The binding sites of MsepCSP5 to candidate volatiles were well predicted by three-dimensional structure modeling and molecular docking experiments. Pursuing further, biological activities of M. separata to highly bound compounds elicited strong behavioral responses, such as alcoholic compounds displayed strong attractiveness whereas terpenes showed repellency to M. separata. The transcript expression level of MsepCSP5 gene significantly decreased after injecting target dsRNAs, and resulted in non-significant preference responses of M. separata to semiochemicals, such as 3-pentanol and 1-octene-3-ol. In conclusion, MsepCSP5 may involve in semiochemical reception of M. separata.

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“…The development not only of refined testing for AMA but also the addition of other PBC-defining autoantibodies to the repertoire has led to an increasing proportion of PBC patients displaying autoantibodies and likely also to earlier diagnosis. Today, even non-invasive testing for AMA seems feasible as a recently published approach to test AMA in saliva has demonstrated ( 81 ). It remains unclear whether these autoantibodies are pathogenetically relevant.…”

Section: Autoantibodies In Pbcmentioning

confidence: 99%

Autoantibodies in Autoimmune Liver Disease—Clinical and Diagnostic Relevance

et al. 2018

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Testing for liver-related autoantibodies should be included in the workup of patients with hepatitis or cholestasis of unknown origin. Although most of these autoantibodies are not disease specific, their determination is a prerequisite to diagnose autoimmune hepatitis (AIH) and primary biliary cholangitis (PBC), and they are components of the diagnostic scoring system in these diseases. In primary sclerosing cholangitis (PSC), on the other hand, autoantibodies are frequently present but play a minor role in establishing the diagnosis. In PSC, however, data on antibodies suggest a link between disease pathogenesis and the intestinal microbiota. This review will focus on practical aspects of antibody testing in the three major autoimmune liver diseases AIH, PBC, and PSC.

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Detection of AMA-M2 in human saliva: Potentials in diagnosis and monitoring of primary biliary cholangitis

Cited by 15 publications

References 43 publications

Dysbiosis of oral microbiota and its association with salivary immunological biomarkers in autoimmune liver disease

Dysbiosis of oral microbiota and its association with salivary immunological biomarkers in autoimmune liver disease

A chemosensory protein MsepCSP5 involved in chemoreception of oriental armyworm Mythimna separata

Autoantibodies in Autoimmune Liver Disease—Clinical and Diagnostic Relevance

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