Context.Recently an unidentified emission line at 3.55 keV has been detected in X-ray spectra of clusters of galaxies. The line has been discussed as a possible decay signature of 7.1 keV sterile neutrinos, which have been proposed as a dark matter (DM) candidate. Aims. We aim to put constraints on the proposed line emission in a large sample of Chandra-observed clusters and obtain limits on the mixing angle in a 7.1 keV sterile neutrino DM scenario. Methods. For a sample of 33 high-mass clusters of galaxies, we merge all observations from the Chandra data archive. Each cluster has more than 100 ks of combined exposure. The resulting high signal-to-noise spectra are used to constrain the flux of an unidentified line emission at 3.55 keV in the individual spectra and a merged spectrum of all clusters. Results. We obtained very detailed spectra around the 3.55 keV range and limits on an unidentified emission line. Assuming all DM were made of 7.1 keV sterile neutrinos, the upper limits on the mixing angle are sin 2 (2Θ) <10.1 × 10 −11 from ACIS-I and <40.3 × 10 −11 from ACIS-S data at 99.7 per cent confidence level. Conclusions. We do not find evidence for an unidentified emission line at 3.55 keV. The sample extends the list of objects searched for an emission line at 3.55 keV and will help to identify the best targets for future studies of the potential DM decay line with upcoming X-ray observatories like Hitomi (Astro-H), eROSITA, and Athena.