2014
DOI: 10.5433/1679-0359.2014v35n5p2539
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Detection of an untyped strain of bovine respiratory syncytial virus in a dairy herd

Abstract: Bovine respiratory syncytial virus (BRSV) causes important lower respiratory tract illness in calves. According to F and G proteins genetic sequences, three BRSV subgroups have been reported and characterized in several countries, showing differences in its distribution. In Brazil, the virus is widely disseminated throughout the herds and the few characterized isolates revealed the solely occurrence of the subgroup B. This study describes the detection and characterization of an untyped BRSV strain from a twen… Show more

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Cited by 5 publications
(3 citation statements)
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“…1c ) differ between human and bovine RSV F (N63K and Q202E). The other main difference between the two RSV F molecules is the presence of an N-linked glycosylation site at N70 in hRSVF that is absent in the bovine counterpart for the strain used in this study 37 (Figs. 1a, c ).…”
Section: Resultsmentioning
confidence: 99%
“…1c ) differ between human and bovine RSV F (N63K and Q202E). The other main difference between the two RSV F molecules is the presence of an N-linked glycosylation site at N70 in hRSVF that is absent in the bovine counterpart for the strain used in this study 37 (Figs. 1a, c ).…”
Section: Resultsmentioning
confidence: 99%
“…Following a positive ELISA, lung homogenates were added to the cell culture and observed daily until CPE including syncytia and round apoptotic cells were visible. The isolation of BRSV in culture can be challenging because the virus is relatively labile and can struggle to grow in culture; however, this successful isolation now opens the door to extensive studies that would not have been possible otherwise [25][26][27].…”
Section: Discussionmentioning
confidence: 99%
“…BRSV infection in calves is widespread in America [20,21] , many European countries [1,8,[22][23][24] and Turkey according to serologic [10,11] and virologic [2] evidence. There is only one study on the investigation with virological methods of BRSV and also other some viruses causing the respiratory disease in calves.…”
Section: Discussionmentioning
confidence: 99%