Detection of anti-citrullinated protein antibody (ACPA) in saliva for rheumatoid arthritis using DBA mice infected with Porphyromonas gingivalis

Sakaguchi, Wakako; To, Masahiro; Yamamoto, Yuko; Inaba, Keitaro; Yakeishi, Mayumi; Saruta, Juri; Fuchida, Shinya; Hamada, Naoki; Tsukinoki, Keiichi

doi:10.1016/j.archoralbio.2019.104510

Cited by 17 publications

(17 citation statements)

References 35 publications

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“…ACPA is highly specific to RA and can be early detected, even before the onset of RA. Therefore, it is used as a specific diagnostic marker for RA [ 38 ]. Recently, it was also found that the pathogenic ACPA are hyperglycosylated which might be regulated by Th17 cells [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Ameliorated Autoimmune Arthritis and Impaired B Cell Receptor-Mediated Ca2+ Influx in Nkx2-3 Knock-out Mice

Khanfar

Olasz

Gábris

et al. 2020

IJMS

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B cells play a crucial role in the pathogenesis of rheumatoid arthritis. In Nkx2-3-deficient mice (Nkx2-3−/−) the spleen’s histological structure is fundamentally changed; therefore, B cell homeostasis is seriously disturbed. Based on this, we were curious, whether autoimmune arthritis could be induced in Nkx2-3−/− mice and how B cell activation and function were affected. We induced arthritis with immunization of recombinant human proteoglycan aggrecan G1 domain in Nkx2-3−/− and control BALB/c mice. We followed the clinical picture, characterized the radiological changes, the immune response, and intracellular Ca2+ signaling of B cells. Incidence of the autoimmune arthritis was lower, and the disease severity was milder in Nkx2-3−/− mice than in control BALB/c mice. The radiological changes were in line with the clinical picture. In Nkx2-3−/− mice, we measured decreased antigen-induced proliferation and cytokine production in spleen cell cultures; in the sera, we found less anti-CCP-IgG2a, IL-17 and IFNγ, but more IL-1β, IL-4 and IL-6. B cells isolated from the lymph nodes of Nkx2-3−/− mice showed decreased intracellular Ca2+ signaling compared to those isolated from BALB/c mice. Our findings show that the transcription factor Nkx2-3 might regulate the development of autoimmune arthritis most likely through modifying B cell activation.

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Section: Discussionmentioning

confidence: 99%

Ameliorated Autoimmune Arthritis and Impaired B Cell Receptor-Mediated Ca2+ Influx in Nkx2-3 Knock-out Mice

Khanfar

Olasz

Gábris

et al. 2020

IJMS

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“…which induce citrullination of different autoantigens (levels of anti-citrullinated protein antibodies are considerably higher in RA patients with than in those without periodontal disease) [143]. These results were recently confirmed on a mice model [144]. Dysregulation would induce tissue destruction and bone resorption.…”

Section: P Gingivalis and Bone Diseasesmentioning

confidence: 85%

Porphyromonas gingivalis outside the oral cavity

et al. 2021

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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“…The index was scored as 0, no change; 1, finger swelling; 2, finger and foot swelling; 3, whole foot swelling; 4, and severe swelling. This was based on the same technique as previously reported [30].…”

Section: Ra Inductionmentioning

confidence: 99%

“…[26,27]. Proteome analysis is currently used to study biomarkers for various diseases such as cancer and autoimmune diseases, leading to early detection of these diseases [28][29][30][31]. In this paper, we report a proteomic analysis of the expression of proteins related to citrullination in saliva using DBA mice in a comprehensive manner.…”

Section: Introductionmentioning

confidence: 99%

Detection of Novel Biomarkers in Saliva of Rheumatoid Arthritis Model Mice by Proteomics and Pathological Analysis

Sakaguchi¹,

Saruta²,

Fuchida³

et al. 2021

Preprint

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Rheumatoid arthritis (RA) is an autoimmune disease in which joints are gradually de-stroyed, and early diagnosis and treatment before joint deformation or destruction is im-portant. The detection of novel RA biomarkers in saliva may facilitate the early detection of RA before the onset of disease. In this study, we conducted a comprehensive proteomic analysis of salivary proteins from RA model mice. Proteins were identified by Western blotting and enzyme-linked immunosorbent assay in serum, saliva, and ankle joints from DBA/1JJmsSlc mice, a model of rheumatoid arthritis. Ankle joints and submandibular glands were hematoxylin and eosin stained and immunostained, and the results were compared with those of control mice. Citrullinated α1 antitrypsin (A1AT, 46 kDa) was commonly detected in saliva, serum, and ankle joints of severe RA model mice, and was confirmed by proteome analysis. Western blotting detected a band corresponding to 46 kDa in serum, saliva and ankle joints, and immunostaining of ankle joints with A1AT an-tibody showed a strong positive signal in the synovium. In DBA/1JJmsSlc mice, not only cyclic citrullinated peptide antibodies but also A1AT may be involved in protein citrulli-nation and contribute to the development and severity of RA.

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Detection of anti-citrullinated protein antibody (ACPA) in saliva for rheumatoid arthritis using DBA mice infected with Porphyromonas gingivalis

Cited by 17 publications

References 35 publications

Ameliorated Autoimmune Arthritis and Impaired B Cell Receptor-Mediated Ca2+ Influx in Nkx2-3 Knock-out Mice

Ameliorated Autoimmune Arthritis and Impaired B Cell Receptor-Mediated Ca2+ Influx in Nkx2-3 Knock-out Mice

Porphyromonas gingivalis outside the oral cavity

Detection of Novel Biomarkers in Saliva of Rheumatoid Arthritis Model Mice by Proteomics and Pathological Analysis

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