Detection of anti-feline infectious peritonitis virus activity of a Chinese herb extract using geneLEAD VIII, a fully automated nucleic acid extraction/quantitative PCR testing system

NISHIJIMA, Rui; Endo, Takuro; GANKHUYAG, Enkhjavkhlan; KHIN, Shwe Thiri Maung Maung; JAFAR, Sheikhy Mohammad; Shinohara, Yuta; Tanaka, Yoshikazu; Sawakami, Kazumi; YODA, Masafumi; Furuya, Tetsuya

doi:10.1292/jvms.22-0185

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Several anti-malarial drugs have been investigated for their antiviral effects. An anti-malarial Chinese herbal extract of unknown identity was found to inhibit the in vitro growth of FIP-associated FCoV [ 391 ]. Chloroquine is too toxic for cats [ 340 , 349 ], and hydroxychloroquine, although used in in vitro studies only, has been suggested as a less-toxic alternative to chloroquine [ 350 ].…”

Section: Treatment Of Fipmentioning

confidence: 99%

Feline Infectious Peritonitis: European Advisory Board on Cat Diseases Guidelines

Tasker,

Addie,

Egberink

et al. 2023

Viruses

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Feline coronavirus (FCoV) is a ubiquitous RNA virus of cats, which is transmitted faeco-orally. In these guidelines, the European Advisory Board on Cat Diseases (ABCD) presents a comprehensive review of feline infectious peritonitis (FIP). FCoV is primarily an enteric virus and most infections do not cause clinical signs, or result in only enteritis, but a small proportion of FCoV-infected cats develop FIP. The pathology in FIP comprises a perivascular phlebitis that can affect any organ. Cats under two years old are most frequently affected by FIP. Most cats present with fever, anorexia, and weight loss; many have effusions, and some have ocular and/or neurological signs. Making a diagnosis is complex and ABCD FIP Diagnostic Approach Tools are available to aid veterinarians. Sampling an effusion, when present, for cytology, biochemistry, and FCoV RNA or FCoV antigen detection is very useful diagnostically. In the absence of an effusion, fine-needle aspirates from affected organs for cytology and FCoV RNA or FCoV antigen detection are helpful. Definitive diagnosis usually requires histopathology with FCoV antigen detection. Antiviral treatments now enable recovery in many cases from this previously fatal disease; nucleoside analogues (e.g., oral GS-441524) are very effective, although they are not available in all countries.

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Section: Treatment Of Fipmentioning

confidence: 99%

Feline Infectious Peritonitis: European Advisory Board on Cat Diseases Guidelines

Tasker,

Addie,

Egberink

et al. 2023

Viruses

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show abstract

“…Recent advancements in understanding the molecular biology of FIPV and therapeutic interventions have provided new insights into the management and potential eradication of the disease. A recent study has highlighted the antiviral activity of an extract from the d3 Chinese herb against FIPV, suggesting a promising avenue for natural component-based therapies (Nishijıma et al, 2023). Furthermore, flavonoids such as isoginkgetin and luteolin have been found to inhibit FIPV replication and exhibit virucidal effects, providing a foundation for the development of flavonoid-based antiviral drugs (Triratapiban et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Theoretical studies of phytochemicals with feline infectious peritonitis virus proteins: a search for novel antivirals

Kurt

2024

International Journal of Agriculture Environment and Food Sciences

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Feline Infectious Peritonitis Virus (FIPV) is a highly lethal pathogen affecting cats worldwide. Developing effective antiviral treatments is crucial for managing this disease. This study investigates the potential of flavonoids to act as antiviral agents and allosteric modulators against the FIPV spike protein using molecular docking simulations. Thirteen flavonoids were docked against the FIPV spike protein (PDB ID: 6JX7) in both ligand-free (cleaned) and ligand-bound (uncleaned) states to assess their binding affinities and potential allosteric effects. The docking results revealed that all tested flavonoids exhibited strong binding affinities, with docking scores ranging from -7.9 to -9.6 kcal/mol in the cleaned receptor state. Notably, Hesperidin, Morin, Hesperetin, and Quercetin maintained or even improved their binding affinities in the presence of native ligands, suggesting their potential as allosteric modulators. Comparative analysis of the binding modes in the cleaned and uncleaned receptor states further supports the allosteric modulator potential of Morin, Hesperetin, and Hesperidin. These findings highlight the promising role of flavonoids as antiviral agents and allosteric modulators targeting the FIPV spike protein. Further experimental validation and optimization of these compounds could lead to the development of effective treatments for feline infectious peritonitis. This study provides valuable insights into the application of flavonoids in the management of viral diseases and contributes to the ongoing efforts in antiviral drug discovery.

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“…Therefore, efforts have been made to develop rapid, portable, and point-of-care devices that can provide nucleic acid test results. Many researchers are currently focusing on developing all-in-one nucleic acid testing devices that are faster, smaller, and more automated [28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection

Wang,

Zhou

et al. 2023

Biosensors

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Pathogenic pathogens invade the human body through various pathways, causing damage to host cells, tissues, and their functions, ultimately leading to the development of diseases and posing a threat to human health. The rapid and accurate detection of pathogenic pathogens in humans is crucial and pressing. Nucleic acid detection offers advantages such as higher sensitivity, accuracy, and specificity compared to antibody and antigen detection methods. However, conventional nucleic acid testing is time-consuming, labor-intensive, and requires sophisticated equipment and specialized medical personnel. Therefore, this review focuses on advanced nucleic acid testing systems that aim to address the issues of testing time, portability, degree of automation, and cross-contamination. These systems include extraction-free rapid nucleic acid testing, fully automated extraction, amplification, and detection, as well as fully enclosed testing and commercial nucleic acid testing equipment. Additionally, the biochemical methods used for extraction, amplification, and detection in nucleic acid testing are briefly described. We hope that this review will inspire further research and the development of more suitable extraction-free reagents and fully automated testing devices for rapid, point-of-care diagnostics.

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Detection of anti-feline infectious peritonitis virus activity of a Chinese herb extract using geneLEAD VIII, a fully automated nucleic acid extraction/quantitative PCR testing system

Cited by 4 publications

References 12 publications

Feline Infectious Peritonitis: European Advisory Board on Cat Diseases Guidelines

Feline Infectious Peritonitis: European Advisory Board on Cat Diseases Guidelines

Theoretical studies of phytochemicals with feline infectious peritonitis virus proteins: a search for novel antivirals

Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection

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