1992
DOI: 10.1007/bf01309815
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Detection of anti-gag antibodies of feline immunodeficiency virus in cat sera by enzyme-linked immunosorbent assay

Abstract: Using gag protein of feline immunodeficiency virus (FIV) expressed in Escherichia coli, an enzyme-linked immunosorbent assay (ELISA) system was developed for detection of antibodies to FIV gag protein in cat sera. With serum samples from cats experimentally infected with several strains and an infectious molecular clone of FIV, increases of the antibody titers to FIV gag protein were observed in all cases by the ELISA at early stage of infection. When we examined a total of 415 field cat sera which were previo… Show more

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Cited by 22 publications
(11 citation statements)
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“…Recently we reported that neutralizing antibodies against FIV Petaluma strain using the sera of Cat 105 were rather high [15]. Furuya et al [12] reported that some serum samples from FIV-infected cats were positive by IFA using FIV-infected MYA-1 cells but negative by enzyme-linked immunosorbent assay using the expressed Gag antigen. Radioimmunoprecipitation assay revealed that those sera had antibodies reactive to envelope protein but not to Gag protein [12].…”
Section: Discussionmentioning
confidence: 92%
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“…Recently we reported that neutralizing antibodies against FIV Petaluma strain using the sera of Cat 105 were rather high [15]. Furuya et al [12] reported that some serum samples from FIV-infected cats were positive by IFA using FIV-infected MYA-1 cells but negative by enzyme-linked immunosorbent assay using the expressed Gag antigen. Radioimmunoprecipitation assay revealed that those sera had antibodies reactive to envelope protein but not to Gag protein [12].…”
Section: Discussionmentioning
confidence: 92%
“…Antibody titer increased in parallel with virus replication, and remained to be high [12]. However, the final phase of disease progression, as suggested by comparison of plasma viral load of Cats 103 and 104 with that of Cat 105, active virus replication was led again when suppression of bone marrow and immunological disorders were progressed.…”
Section: Discussionmentioning
confidence: 93%
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“…Each of these experimental cats was infected orally with 10,000 bradyzoites of the T. gondii Beverley strain. A total of 187 field cat sera were collected from domestic cats, which had been brought to veterinary hospitals near Tokyo and Sapporo for treatment, as described previously (6,7).…”
Section: Methodsmentioning
confidence: 99%
“…Although pattems of antibody reactivity to specific viral antigens are not as well characterized in lentiviral infections of domestic animals, there is some evidence for a similar link between a loss of antibodies to viral core antigens and disease development in feline immunodeficiency virus (FIV) (12,15) and visna virus (19) infections.…”
Section: Introductionmentioning
confidence: 99%