Detection of anti-HLA antibodies by ELISA: relevance to heart transplantation

Bishara, Amal; Admon, Dan; Sherman, L; Brautbar, Chaim

doi:10.1016/s0041-1345(01)02247-3

Cited by 2 publications

(3 citation statements)

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“…Such antibodies have been associated with rejection of solid organ transplants. [24][25][26] Investigators have reported extracorporeal immunoadsorption as a method for decreasing anti-HLA antibodies in organ transplant recipients. 27 In our experience, the use of anti-CD20 antibody and plasmapheresis, followed by reconditioning with fludarabine and TLI led to successful engraftment after a second haploidentical infusion from the same donor.…”

Section: Discussionmentioning

confidence: 99%

Effective donor lymphohematopoietic reconstitution after haploidentical CD34+-selected hematopoietic stem cell transplantation in children with refractory severe aplastic anemia

Woodard

Cunningham

Benaim

et al. 2003

Bone Marrow Transplant

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Summary:Peritransplant toxicity and a delay in effective immune reconstitution have limited the utility of alternate donor transplantation for children with refractory severe aplastic anemia. We have assessed the effectiveness of infusing large numbers of highly purified haploidentical CD34 þ cells after immunoablative conditioning in three patients who had failed intensive immunosuppression, lacked unrelated donors, and had active or recent serious infections. One patient rejected the first infusion, but engrafted after a second infusion from the same donor. This patient died 4 months after hematopoietic stem cell transplantation with no evidence of lymphoid reconstitution. Two patients experienced mixed chimerism requiring treatment with antibodies and/or donor lymphocyte infusion. Both currently survive more than 1 year after transplantation with normal blood counts, 100% donor engraftment, effective lymphoid reconstitution, and no chronic graft-versus-host disease. We observed functional thymopoiesis as measured by lymphocyte immunophenotyping, T cell receptor excision circles and T cell receptor Vb spectratyping complexity analysis. Further study is required to validate the initial promise of these preliminary observations.

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Section: Discussionmentioning

confidence: 99%

Effective donor lymphohematopoietic reconstitution after haploidentical CD34+-selected hematopoietic stem cell transplantation in children with refractory severe aplastic anemia

Woodard

Cunningham

Benaim

et al. 2003

Bone Marrow Transplant

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“…The class distribution of anti-HLA antibody after transplantation was: 5 Class I, 2 Class II, and 12 Class I ϩ II. De novo antibodies included 4 Class I, 6 Class I ϩ II, and 1 Class II (Table 1) …”

Section: Elisa-detected Anti-hla Antibodiesmentioning

confidence: 99%

“…Preformed anti-human leukocyte antigen (HLA) antibodies have been associated with hyperacute rejection, humoral rejection, and increased incidence of acute cellular rejection [3][4][5][6][7][8]. More recently, some authors also emphasized a correlation between preformed antibodies and chronic rejection, including in heart transplantation [7,8].…”

Section: Introductionmentioning

confidence: 99%