Detection of anti‐lens crystallin antibody in dogs with and without cataracts

Denis, H. M.; Brooks, Dennis; Alleman, A. Rick; Andrew, Stacy E.; Plummer, Caryn E.

doi:10.1111/j.1463-5224.2003.00314.x

Cited by 13 publications

(12 citation statements)

References 24 publications

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“…Interestingly, in the work of Denis et al about the detection of anti-lens crystalline antibodies in dogs with and without cataract, the authors also did not find a correlation between the presence and maturity of cataract and the presence of anti-lens crystalline serum antibodies [6]. The present study confirms these findings in humans.…”

Section: Discussionsupporting

confidence: 93%

High incidence of antibodies to lens proteins in sera from patients with uveitis

Doycheva

Preuß

Klein

et al. 2006

Graefe's Arch Clin Exp Ophthalmol

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These data indicate that antibodies to lens proteins occur in a high incidence in sera from patients with uveitis. Forty-nine percent of the patients with anterior uveitis and only 12% of healthy controls were positive in the ELISA. In our groups of patients and controls the autoantibodies reacted in the Western blot predominantly with alpha-crystallin. Further studies are required to analyze in more detail the clinical and etiopathogenetic relevance of the antilens antibodies in uveitis.

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Section: Discussionsupporting

confidence: 93%

High incidence of antibodies to lens proteins in sera from patients with uveitis

Doycheva

Preuß

Klein

et al. 2006

Graefe's Arch Clin Exp Ophthalmol

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“…Further, the data do not support a proposed mechanism whereby βH‐crystallins leak from lenses due to membrane breakdown into the serum causing a generalized increased autoimmune reaction against lens proteins resulting in cataract. A similar negative correlation between serum antibodies against general lens crystallins and stage of cataract in an unknown species of dog was also reported, and the data supporting a similar autoimmune mechanism for human cataract are conflicted …”

Section: Discussionsupporting

confidence: 56%

“…Post‐developmental modifications, include truncation, phosphorylation, and deamidation. Another postulated mechanism is that the plasma membranes of the lens leak crystallins into the anterior chamber and systemic circulation, causing an autoimmune reaction to lens proteins, cataract formation, and uveitis . As this latter mechanism is controversial, the purpose of this study was to determine the relationship between serum antibodies to βH‐crystallins and the stage of cataract in ACS.…”

Section: Introductionmentioning

confidence: 99%

Serum antibodies against βH‐crystallins in the American Cocker Spaniel

Kanemaki

Fukiage

Ichikawa

et al. 2013

Veterinary Ophthalmology

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ObjectiveTo detect antibodies for lens βH-crystallins in the serum from the American Cocker Spaniel (ACS) presenting with and without cataracts and with and without uveitis.Animal StudiedSeventy-three American Cocker Spaniels and six normal Beagles.ProceduresSera were collected from 73 ACSs, including those with normal lenses and those with cataracts, or uveitis. Fractionated, normal Beagle lens βH-crystallins were separated by one- or two-dimensional electrophoresis. The separated lens βH-crystallins were used on immunoblots as sentinel substrates against which the ACS sera were tested for the presence of antibodies against βH-crystallins.ResultsSera from approximately two-thirds of study animals contained antibodies to some βH-crystallin polypeptides, but reactivity varied among patients. Contrary to some hypotheses, serum antibodies to groups of βH-crystallins did not relate to the stages of cataract. However, detailed analysis by two-dimensional immunoblotting and mass spectrometry showed that three spots originating from βA1-crystallin were detected only in sera from cataract patients.ConclusionSerum antibodies to βA1-crystallin may be associated with the development of cataract.

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“…For instance, crystallins leaking from lens fiber cells through intact capsules can cause severe eye inflammation (55). EGF, IGF, and insulin are vital for proper lens cell growth and differentiation (56), while the carrier proteins BSA, transferrin, and ceruloplasmin are responsible for the endo- and transcytotic delivery of the iron, copper, and fatty acids essential for normal cell metabolism to the lens (57–59).…”

Section: Discussionmentioning

confidence: 99%

Characterizing molecular diffusion in the lens capsule

et al. 2010

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The lens capsule compartmentalizes the cells of the avascular lens from other ocular tissues. Small molecules required for lens cell metabolism, such as glucose, salts, and waste products, freely pass through the capsule. However, the lens capsule is selectively permeable to proteins such as growth hormones and substrate carriers which are required for proper lens growth and development. We used fluorescence recovery after photobleaching (FRAP) to characterize the diffusional behavior of various sized dextrans (3, 10, 40, 150, and 250kDa) and proteins endogenous to the lens environment (EGF, γD-crystallin, BSA, transferrin, ceruloplasmin, and IgG) within the capsules of whole living lenses. We found that proteins had dramatically different diffusion and partition coefficients as well as capsule matrix binding affinities than similar sized dextrans, but they had comparable permeabilities. We also found ionic interactions between proteins and the capsule matrix significantly influence permeability and binding affinity, while hydrophobic interactions had less of an effect. The removal of a single anionic residue from the surface of a protein, γD-crystallin [E107A], significantly altered its permeability and matrix binding affinity in the capsule. Our data indicated that permeabilities and binding affinities in the lens capsule varied between individual proteins and cannot be predicted by isoelectric points or molecular size alone.

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Detection of anti‐lens crystallin antibody in dogs with and without cataracts

Abstract: There is a negative association between the presence (P = 0.004) and maturity (P = 0.004) of cataract and presence of ALC serum antibodies.

Cited by 13 publications

References 24 publications

High incidence of antibodies to lens proteins in sera from patients with uveitis

High incidence of antibodies to lens proteins in sera from patients with uveitis

Serum antibodies against βH‐crystallins in the American Cocker Spaniel

Characterizing molecular diffusion in the lens capsule

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