Detection of anti‐Ro/SSA and anti‐La/SSB autoantibody‐producing cells in salivary glands from patients with Sjögren's syndrome

Tengnér, Pia; Halse, Anne‐Kristine; Haga, Hans‐Jacob; Jönsson, Roland; Wahren‐Herlenius, Marie

doi:10.1002/1529-0131(199812)41:12<2238::aid-art20>3.3.co;2-m

Cited by 80 publications

(106 citation statements)

References 7 publications

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“…Anti-Ro/SSA and anti-La/SSB autoantibodyproducing cells have been detected in the MSG of patients with Sjö gren's syndrome. (Tengner et al, 1998).…”

Section: Discussionmentioning

confidence: 73%

Two Different Types of Sialoadenitis in the NOD- and MRL/lpr Mouse Models for Sjögren's Syndrome: A Differential Role for Dendritic Cells in the Initiation of Sialoadenitis?

Blokland

Helden-Meeuwsen

Wierenga‐Wolf

et al. 2000

Laboratory Investigation

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SUMMARY: Sjö gren's syndrome is an autoimmune disease that primarily affects the salivary and lacrimal glands. In these glands, focal lymphocytic infiltrates develop. Little is known about the initiation of this autoimmune disease. Antigen-presenting cells (APC) such as dendritic cells (DC) can play a role in the initiation of autoimmunity. To date, no data on the presence of DC in Sjö gren's syndrome are available. Several mouse strains, the nonobese diabetic (NOD) and the MRL/lpr mouse, can be used as models for Sjö gren's syndrome. We compared the development of sialoadenitis in the submandibular glands (SMG) of NOD and MRL/lpr mice with particular focus on the presence of APC. DC, macrophages, T cells, and B cells in the SMG were studied by means of immunohistochemistry, after which positively stained cells were quantified. NOD-severe combined immunodeficiency (SCID) mice were used to study the presence of APC in the SMG in the absence of lymphocytes. Before lymphocytic infiltration, increased numbers of DC were detected in the SMG of NOD mice compared with those numbers in control mice and MRL/lpr mice, which suggests that DC play a role in the initiation of sialoadenitis in NOD mice. In the SMG of NOD mice, lymphocytic infiltrates organized in time. In MRL/lpr mice, however, lymphocytic infiltrates were already organized at the time of appearance. This organization was lost over time. In conclusion, two types of sialoadenitis are described in two mouse models for Sjö gren's syndrome. Differences exist with regard to early events that may lead to the development of sialoadenitis and to the composition and organization of inflammatory infiltrates. It is possible that different types of sialoadenitis also exist in humans and that the pathogenetic process in both the early and late phases of the autoimmune reaction differs among patients. (Lab Invest 2000, 80:575-585).

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“…Anti-Ro/SSA and anti-La/SSB autoantibodyproducing cells have been detected in the MSG of patients with Sjö gren's syndrome. (Tengner et al, 1998).…”

Section: Discussionmentioning

confidence: 73%

Two Different Types of Sialoadenitis in the NOD- and MRL/lpr Mouse Models for Sjögren's Syndrome: A Differential Role for Dendritic Cells in the Initiation of Sialoadenitis?

Blokland

Helden-Meeuwsen

Wierenga‐Wolf

et al. 2000

Laboratory Investigation

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“…Production and purification of recombinant Ro 52-kd, Ro 60-kd, and La proteins were performed as described previously (28). The proteins were used in ELISA and Western blotting for detection of antibodies as described previously (11).…”

Section: Methodsmentioning

confidence: 99%

Signs of first‐degree heart block occur in one‐third of fetuses of pregnant women with anti–SSA/Ro 52‐kd antibodies

Sonesson

Salomonsson

Jacobsson

et al. 2004

Arthritis & Rheumatism

187

182

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Objective. To prospectively investigate the development of fetal heart block in anti-SSA/Ro 52-kdpositive women, and to evaluate the usefulness of serial Doppler echocardiography in detecting early signs of congenital heart block.Methods. Twenty-four women with anti-SSA/Ro 52-kd antibodies and consequently increased risk for fetal heart block were followed up weekly, between 18 and 24 weeks of gestation, with two Doppler echocardiographic methods designed to estimate the time delay between hemodynamic events caused by atrial and ventricular depolarizations. Two hundred eighty-four women with normal pregnancies served as controls. Anti-Ro 52-kd, anti-Ro 60-kd, and anti-La antibodies were investigated by immunoblotting and enzymelinked immunosorbent assay using recombinant proteins.Results. In anti-Ro 52-kd-positive women, fetal atrioventricular (AV) time intervals were longer and heart rates were slightly lower compared with those in controls. Eight of 24 fetuses had signs of first-degree block. One of these fetuses had progression to complete block, and another showed recovery from second-degree block to first-degree block with betamethasone treatment. In the remaining 6 fetuses, spontaneous normalization occurred before or shortly after birth. Fetuses with normal AV time intervals at 18-24 weeks had normal electrocardiographic results at birth.Conclusion. Anti-Ro 52-kd-positive pregnant women frequently carry fetuses with Doppler echocardiographic signs of first-degree AV block. These blocks revert spontaneously in the majority of fetuses, but progression to a more severe degree of block may occur in some. Serial Doppler echocardiographic measurement of AV time intervals is suggested as a useful method for surveillance of these high-risk pregnancies.

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“…The frequent identification of GCs in salivary glands in SS and the circumstantial evidence in favour of local antibody production [83] would support the hypothesis that MALT lymphoma develops from B cells chronically activated in the glands. Since there is a clear association between MESA and B-cell lymphoma this hypothesis would predict that post GC cells migrate to and into ductal epithelium, perhaps by alteration in chemokine responsiveness mediated by alterations in chemokine receptor expression.…”

Section: Mesa Formation and Malt Lymphomamentioning

confidence: 99%

Chemokines and Cell Trafficking in Sjögren's Syndrome

Amft

Bowman

2001

Scand J Immunol

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Sjögren's syndrome is a chronic inflammatory condition affecting exocrine glands, manifested clinically as dry eyes and dry mouth. It arises secondary to systemic immune‐mediated diseases such as rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), scleroderma or ‘primary’ Sjögren's syndrome. Histologically it is characterized by peri‐ductal aggregates of CD4 T lymphocytes, the frequent occurrence of ectopic germinal centres and, in some patients, B‐cell infiltration of ductal epithelium (myoepithelial sialadenitis). This latter lesion is the precursor for the development of low grade (MALT) B‐cell lymphoma. The identification over recent years of chemokines and their receptors enables us to address the specific processes involved in the migration of inflammatory cells into exocrine glands, the development of their secondary structures and patterns of retention within the glands and potentially the subsequent transformation of B cells into mucosa associated lymphoid tissue (MALT) lymphoma.

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Detection of anti‐Ro/SSA and anti‐La/SSB autoantibody‐producing cells in salivary glands from patients with Sjögren's syndrome

Cited by 80 publications

References 7 publications

Two Different Types of Sialoadenitis in the NOD- and MRL/lpr Mouse Models for Sjögren's Syndrome: A Differential Role for Dendritic Cells in the Initiation of Sialoadenitis?

Two Different Types of Sialoadenitis in the NOD- and MRL/lpr Mouse Models for Sjögren's Syndrome: A Differential Role for Dendritic Cells in the Initiation of Sialoadenitis?

Signs of first‐degree heart block occur in one‐third of fetuses of pregnant women with anti–SSA/Ro 52‐kd antibodies

Chemokines and Cell Trafficking in Sjögren's Syndrome

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