DETECTION OF ANTIBIOTIC RESISTANCE IN Pseudomonas aeruginosa ISOLATES WITH SPECIAL REFERENCE TO METALLO Β-LACTAMASES FROM A TERTIARY CARE HOSPITAL IN WESTERN INDIA

Multidrug-resistant (MDR) bacteria, such as extended-spectrum beta-lactamase (ESBL) Enterobacteriaceae, pose a challenge to human and animal health care systems. Antibiotic resistance in non-lactose fermenting pathogens such as Pseudomonas aeruginosa is increasing, making these clinical pathogens more difficult to treat. The most predominant Pseudomonas species that cause mortality among birds, especially chickens, is P. aeruginosa. The present study detected multi-drug resistance and ESBL-production among P. aeruginosa isolated from broiler chickens in different poultry farms. A total of 450 broiler chickens from 150 private farms were examined for the presence of P. aeruginosa using bacteriological and molecular methods. Samples were collected from the internal organs of the birds and subjected to bacteriological examination and identification. The bacteriological examination revealed that 29 farms (19.33%) were positive for P. aeruginosa. On the other hand, by PCR, only 25 farms (16.66%) were positive. All P. aeruginosa isolates were subjected to in vitro antimicrobial susceptibility testing against 15 antimicrobial agents by the disc diffusion method. P. aeruginosa isolates showed resistance to a wide range of antimicrobial agents, including trimethoprim/sulfamethoxazole (80%), followed by streptomycin (56%), colistin sulfate (48%), ceftazidime (32%), gentamicin and ciprofloxacin (28% each). In contrast, resistance levels to cefotaxime, oxytetracycline, and norfloxacin were 24%, 20%, and 16%, respectively. The lowest resistance was against apramycin (12%), fosfomycin, imipenem, and ertapenem (8%). All P. aeruginosa isolates were sensitive to amikacin and meropenem. The antibiotic-resistant pattern of the isolated P. aeruginosa revealed that the multi-drug resistance (MDR) level was 14/25 (56%). Only 16 isolates (64%) were ESBL producing. From the ESBL isolates, 9/16 (56.25%) were MDR. Molecular detection for antibiotic resistance gene revealed that blaTEM was detected in 20/25 (80%) of P. aeruginosa isolates. From the blaTEM gene-positive P. aeruginosa, 16/20 isolates (80%) were ESBL producers, and 11/20 (55%) were MDR. The results indicated that MDR and ESBL producing P. aeruginosa frequency reached an alarming level in poultry isolates in Egypt. In conclusion, the misuse of antibiotics leads to the development of resistant bacteria that may transfer from poultry to humans. Strict supervision and enforcement of laws to control antibiotic usage in the food chain within established safe levels must be done.

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Comparison of Double-Disc Synergy, Combined Disc Diffusion, and E Test for Metallo-Beta-Lactamase Detection in Pseudomonas Aeruginosa in a Tertiary Care Hospital

et al. 2023

Asian J Pharm Clin Res

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Objective: Pseudomonas aeruginosa is an important opportunistic pathogen associated with nosocomial infections. The metallo-beta-lactamases (MBLs) are an important class of carbapenemases which are predominantly produced by P. aeruginosa. This study is aimed to study the prevalence of MBLs among imipenem (IMP)-resistant P. aeruginosa and to evaluate three different methods of screening and detecting MBLs produced by P. aeruginosa. Methods: 100 isolates of P. aeruginosa were obtained from various clinical specimens, including pus, wound, sputum, urine, body fluids, and ET tips received at the laboratory from July 2021 to December 2021. Screening for MBL production among IMP-resistant P. aeruginosa was done by double-disc synergy test and combined disc test. Confirmation of MBL production was done by E-test. Results: Of the 100 P. aeruginosa isolates, 16 were IMP resistant, of which 16 are MBL producers. Most of the samples were obtained from 50 to 60 years followed by 40–50 and 60–70 were pus (50%) followed by sputum (20%), urine (15%), and body fluids (5%). Of the 100 isolates, 66 were isolated from females and 34 from males. Pus samples showed most MBL-producing isolates, accounting for 60.5%. MBL producers accounted for 90% of IMP-resistant cases using combined disc method and 100% using MBL E-strip test. By comparison, double-disc synergy test (DDST) retrieved 45% of Pseudomonas MBL producers. Conclusion: This study found moderate prevalence of Pseudomonas MBL producers (16/100). The study supports the use of combined disc-diffusion test and DDST for screening and confirming MBL producers of P. aeruginosa by E test where polymerase chain reaction is not available.

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DETECTION OF ANTIBIOTIC RESISTANCE IN Pseudomonas aeruginosa ISOLATES WITH SPECIAL REFERENCE TO METALLO Β-LACTAMASES FROM A TERTIARY CARE HOSPITAL IN WESTERN INDIA

Cited by 3 publications

References 10 publications

Exploring the potential reservoirs of non specific TEM beta lactamase ( bla TEM ) gene in the Indo-Gangetic region: A risk assessment approach to predict health hazards

Exploring the potential reservoirs of non specific TEM beta lactamase ( bla TEM ) gene in the Indo-Gangetic region: A risk assessment approach to predict health hazards

Emergency of extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa isolated from broiler chickens in Egypt

Comparison of Double-Disc Synergy, Combined Disc Diffusion, and E Test for Metallo-Beta-Lactamase Detection in Pseudomonas Aeruginosa in a Tertiary Care Hospital

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