Detection of antibiotic resistance in probiotics of dietary supplements

Abstract

BackgroundProbiotics are live microorganisms that confer nutrition- and health-promoting benefits if consumed in adequate amounts. Concomitant with the demand for natural approaches to maintaining health is an increase in inclusion of probiotics in food and health products. Since probiotic bacteria act as reservoir for antibiotic resistant determinants, the transfer of these genes to pathogens sharing the same intestinal habitat is thus conceivable considering the fact that dietary supplements contain high amounts of often heterogeneous populations of probiotics. Such events can confer pathogens protection against commonly-used drugs. Despite numerous reports of antibiotic resistant probiotics in food and biological sources, the antibiogram of probiotics from dietary supplements remained elusive.FindingsHere, we screened five commercially available dietary supplements for resistance towards antibiotics of different classes. Probiotics of all batches of products were resistant towards vancomycin while batch-dependent resistance towards streptomycin, aztreonam, gentamycin and/or ciprofloxacin antibiotics was detected for probiotics of brands Bi and Bn, Bg, and L. Isolates of brand Cn was also resistant towards gentamycin, streptomycin and ciprofloxacin antibiotics. Additionally, we also report a discrepancy between the enumerated viable bacteria amounts and the claims of the manufacturers.ConclusionsThis short report has highlighted the present of antibiotic resistance in probiotic bacteria from dietary supplements and therefore serves as a platform for further screenings and for in-depth characterization of the resistant determinants and the molecular machinery that confers the resistance.Electronic supplementary materialThe online version of this article (doi:10.1186/s12937-015-0084-2) contains supplementary material, which is available to authorized users.

Antibiotic resistant microencapsulated probiotics synergistically preserved orange juice

“…However, the bacteria counts indicated that all of the samples had lower numbers of probiotics than that declared by their producers (Fig. 1) and the results were consistent with other reports [23,24]. Most importantly, the viable bacteria counts in S4 and S5 were between 100 and 1000 times lower than claimed in their respective datasheets.…”

“…However, this factor alone was not sufficient to explain the large differences in CFUs reached and those claimed. Therefore, other potential problems have occurred: 1) a large number of bacteria were destroyed during different stages of processing and sometimes, the manufacturing companies intentionally exaggerated the contents in their probiotic products [27,28], 2) the product was not dried enough and a high water activity in the powder was detrimental to viability during storage, 3) an efficient antioxidant was absent in the product, 4) packaging did not prevent the entrance of moisture or oxygen in the product during storage, 5) storage temperature was not cold enough or there was indiscriminate handling and storage conditions (cold chains) [23]. It was reported that probiotic products should be consumed regularly, at a daily intake of approximately 10 9 viable cells [29].…”

Antibiotic Susceptibility of Lactobacillus sp. Isolated from Commercial Probiotic Products by E-Test Strip Method

“…The health benefits of consuming these organisms range from improved intestinal health and immunity, prevention of antibiotic associated diarrhea and even cancer. Probiotic foods are having global popularity and widespread acceptability as depicted by an estimated growth of more than 10% in last one decade (Wong et al, 2015). Accelerated by increased consumer demand, these health beneficial microorganisms have been comprehensively…”

Dut Yaprağından Elde Edilen Flavonoidler ile Candida Tropicalis’in Buzağılarda Performans ve Besin Sindirilebilirliği Üzerine Etkileri

“…Probiotics are live microorganisms including bacteria and yeasts, which can improve intestinal health and immune response, prevent acute and antibiotic-associated diarrhea [9] . It is reported that GSY10 is the most promising oleaginous yeast for microbial lipid production from molasses, and it can be used as feed supplement for microbial lipid production in dairy cattle [10] .…”