“…At the present, rivers are highly contaminated with antibiotic-resistant bacteria, transferred from sewage drainage, especially from hospitals, agricultural drainage, slaughterhouses, clinics, and animal husbandries (Djenadi, 2017). Recently, (Zaki et al, 2023) found antibioticresistant bacteria in the Nile River's raw and tap water in the Dakahlia Region of Egypt due to urbanization, accumulation of microbial contamination during different water transfers from source to homes, misuse and increased exposure to antibiotics in human treatment, in poultry, and livestock farms and agriculture, which may pose a serious ecological risk to the waters and public health. Therefore, careful use of antibiotics should be applied to counteract the rising problem of multidrug resistant P. putida that is both survive tough environments and zoonotic.…”