Detection of Antibodies Against Capripoxviruses Using an Inactivated Sheeppox Virus ELISA

Babiuk, Shawn; Wallace, David B.; Smith, S J; Bowden, Timothy R.; Dalman, Brett; Parkyn, Geoff; Copps, John; Boyle, D. B.

doi:10.1111/j.1865-1682.2009.01067.x

Cited by 81 publications

(55 citation statements)

References 28 publications

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“…Capripoxvirus neutralizing antibodies were measured in sheep sera using Micro-neutralisation test (MNT) [10,2]. A total of 30 sera samples were tested for antibodies to SPPV and GTPV by the MNT.…”

Section: Methodsmentioning

confidence: 99%

Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32

Tian

Chen

et al. 2010

Virol J

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BackgroundSheeppoxvirus (SPPV), goatpoxvirus (GTPV) and lumpy skin disease virus (LSDV) of cattle belong to the Capripoxvirus genus of the Poxviridae family and can cause significant economic losses in countries where they are endemic. Despite the considerable threat that these viruses pose to livestock production and global trade in sheep, goats, cattle and their products, convenient and effective serodiagnostic tools are not readily available. Toward this goal, two synthetic peptides corresponding to the major antigen P32 were synthesized. These synthetic peptides were then used as antigen to develop an ELISA method to detect anti-SPPV and GTPV antibodies.ResultsThe results indicated that the optimal concentration of coated recombinant antigen was 0.2 μg per well for a serum dilution of 1:10. The ELISA performed favorably when sera from sheep immunized experimentally were tested.ConclusionThis assay offers the prospect of synthetic peptide as antigens for indirect ELISA to detect SPPV and GTPV antibody in sheep and goat sera.

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Section: Methodsmentioning

confidence: 99%

Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32

Tian

Chen

et al. 2010

Virol J

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“…The earliest ELISA developed for capripoxviruses utilized a protein encoded by P32 (vaccinia H3L homologue) as an antigen (Carn et al., 1994; Heine et al., 1999). More recently, an indirect ELISA was developed based on whole heat‐inactivated sheep pox virus as an antigen (Babiuk et al., 2009). When 276 cattle serum samples were tested, the diagnostic sensitivity and specificity of this assay were 88% and 97%, respectively.…”

Section: Novel Diagnostic Testsmentioning

confidence: 99%

Review: Lumpy Skin Disease: An Emerging Threat to Europe, the Middle East and Asia

Tuppurainen

Oura

2011

Transboundary and Emerging Diseases

354

416

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Lumpy skin disease (LSD) is an economically devastating emerging viral disease of cattle. Lumpy skin disease is currently endemic in most African countries and has recently spread out of Africa into the Middle East region. In this article, we review the putative mechanisms of spread of LSD into the Middle East and the risks of further spread into Turkey, Europe and Asia. We also review the latest findings on the epidemiology of LSD, its mechanisms of transmission, the potential role of wildlife in its maintenance and spread and the diagnostic tests and control methods currently available.

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“…The sensitivity of neutralisation assays varies between 70 and 96 % and specificity can reach 100 % (Sadri et al, 2002;Gari et al, 2008;Babiuk et al, 2009). They are very specific but not sufficiently sensitive for the detection of low antibody titres in animals with mild clinical disease and vaccinated animals; they are too labour-intensive and time-consuming; and they are not suitable for primary assay or testing large number of samples.…”

Section: Detection Of Antibodies Against Lsdvmentioning

confidence: 99%

Scientific Opinion on lumpy skin disease

2015

EFSA Journal

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Lumpy skin disease (LSD) is a viral disease of cattle characterised by severe losses, especially in naive animals. LSD is endemic in many African and Asian countries, and it is rapidly spreading throughout the Middle East, including Turkey. LSD is transmitted by mechanical vectors, but direct/indirect transmission may occur. The disease would mainly be transferred to infection-free areas by transport of infected animals and vectors. In the EU, it could only happen through illegal transport of animals. The risk for that depends on the prevalence in the country of origin and the number of animals illegally moved. Based on a model to simulate LSD spread between farms, culling animals with generalised clinical signs seems to be sufficient to contain 90 % of epidemics around the initial site of incursion, but the remaining 10 % of simulated epidemics can spread up to 400 km from the site of introduction by six months after incursion. Whole-herd culling of infected farms substantially reduces the spread of LSD virus, and the more rapidly farms are detected and culled, the greater the magnitude of the reduction is. Only live attenuated vaccines against LSD are available. Homologous vaccines are more effective than sheep pox strain vaccines. The safety of the vaccines should be improved and the development of vaccines for differentiating between infected and vaccinated animals is recommended. Epidemics are not self-limiting when effective vaccination or culling are not applied. Active surveillance, rapid detection and prompt culling of infected herds are effective measures for LSD control. The role of vectors for LSD transmission should be further investigated in both controlled environments and the field. Awareness-raising campaigns for farmers and veterinary staff to promote recognition of LSD should be considered. The cooperation of the EU with neighbouring countries should be encouraged to prevent transboundary disease spread.

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Detection of Antibodies Against Capripoxviruses Using an Inactivated Sheeppox Virus ELISA

Cited by 81 publications

References 28 publications

Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32

Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32

Review: Lumpy Skin Disease: An Emerging Threat to Europe, the Middle East and Asia

Scientific Opinion on lumpy skin disease

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