Detection of antibodies against porcine parvovirus nonstructural protein NS1 may distinguish between vaccinated and infected pigs

Madsen, E. S.; Madsen, K.G.; Nielsen, Jens Peter; Jensen, Merethe Holm; Lei, J.C.; Have, P.

doi:10.1016/s0378-1135(96)01270-9

Cited by 25 publications

(15 citation statements)

References 17 publications

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“…The lower bands may represent degraded protein. Other researchers have also reported the rapid degradation of the NS1 protein of PPV (Madsen et al, 1997) and ADV (Christensen et al, 1995). The two minor bands (60 and 43 kDa) did not react with the PPV-positive sera in the Western blotting test.…”

Section: Discussionmentioning

confidence: 90%

“…In this study, we obtained a high yield of rNS1 proteins (86 kDa) from a prokaryotic expression system and veri¢ed its high antigenicity. The rNS1 protein reacted with the sera samples from PPV-infected pigs, but not those from inactivated virus-vaccinated pigs, probably because NS1 is not expressed from the inactivated virus vaccines or because the amount contained in the vaccine was too low to induce detectable antibody response (Madsen et al, 1997;Lv, 2002). When PPV-NS1 antigen was expressed in an E. coli expression system, three di¡erent forms of rNS1 protein with observed molecular weights of about 86, 60 and 43 kDa were found in SDS-PAGE.…”

Section: Discussionmentioning

confidence: 99%

“…The NS1 protein of PPV was originally described as a soluble complement-¢xing antigen. It may also be a circulating antigen that plays an important role in mediating immunity after active infection (Madsen et al, 1997;Lv, 2002;Jauling et al, 2001). Madsen and colleagues (1997) used a baculovirus expression system to express PPV NS1 protein, which is more expensive and complicated than a prokaryotic expression system.…”

Section: Discussionmentioning

confidence: 99%

“…Detection of parvovirus B19 NS1 speci¢c antibody is useful in determining the level of protection from a capsid-based vaccine, as the emergence of anti-NS1 antibodies indicates parvovirus infection (Heegaard et al, 2002). Since the inactivated PPV vaccines that consist of semi-puri¢ed, chemically inactivated virus can elicit only antibodies against the structural proteins, assays that speci¢cally detect antibodies against the NS1 proteins may be useful in di¡erentiating vaccinated from infected animals (Madsen et al, 1997;Lv, 2002).…”

Section: Introductionmentioning

confidence: 99%

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The Recombinant Nonstructural Polyprotein NS1 of Porcine Parvovirus (PPV) as Diagnostic Antigen in ELISA to Differentiate Infected from Vaccinated Pigs

Qing

et al. 2006

Vet Res Commun

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To differentiate pigs infected with porcine parvovirus (PPV) from those vaccinated with inactivated whole-virus vaccine, an enzyme-linked immunosorbent assay (ELISA) based on detection of a nonstructural polyprotein 1 (NS1) was developed. A threshold of 0.23 optical density units was established and the assay had high specificity (100), sensitivity (88), accuracy (90) and positive predictive value (100) using haemagglutination inhibition as the standard method. A reproducibility test revealed that the coefficients of variation of sera within-plates and between-run were less than 10%. The assay showed no cross-reactivity with antibodies to porcine reproductive respiratory syndrome virus, pseudorabies virus, foot and mouth disease virus, Actinobacillus pleuropneumoniae, Toxoplasma or Chlamydia. Sera obtained from pigs infected with PPV reacted with recombinant NS1 protein in the ELISA. Sera from pigs vaccinated with inactivated whole virus did not recognize this protein in the ELISA. In contrast, antibodies against PPV whole virus were present in both PPV-infected and vaccinated animals. Serum conversion against NS1 was first detected 10 days after infection and NS1-specific antibodies were detectable up to half a year post infection. In conclusion, the PPV-NS1 ELISA can differentiate PPV-infected versus inactivated PPV-vaccinated pigs and could be applied in disease diagnosis and surveillance.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Recombinant Nonstructural Polyprotein NS1 of Porcine Parvovirus (PPV) as Diagnostic Antigen in ELISA to Differentiate Infected from Vaccinated Pigs

Qing

et al. 2006

Vet Res Commun

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show abstract

“…In weeks 9, 12 and 14, whole blood was collected in plane tubes and stored at room temperature for 6 to 10 h until centrifugation at 1850Âg for 20 min. The serum was separated from the blood corpuscles and stored at À 20 8C until analysis for specific antibodies to PPV using a blocking ELISA (Madsen et al, 1997).…”

Section: Immunological Assessmentmentioning

confidence: 99%