Detection of antibodies bound to tumor cell surface antigens with radioiodinated Staphylococcus aureus protein A (SPA)

Nayak, Shankar K.; Knotts, F. Barry; Drogemuller, Carol R.; Pilch, Yosef H.

doi:10.1007/bf00200147

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…Other authors investigating the IgG uptake by can cer cells have suggested the participation of natural antibodies [29], Forssman-like antibodies [11], auto antibodies or allo-antibodies [15] on the part of the serum, as well as Fc-like receptors on the part of the cells [10,22,24]. Judging by our own studies, it would appear possible that surface binding of autologous and allogeneic IgG by cancer cells may at least partly be of a non-specific, perhaps even non-immune type due to the dynamic conditions of the external cancer-cell plasma membranes which are highly associated with glycoproteins and mucopolysaccharides [12,16] …”

Section: '25i-s[)a Binding and Re-binding O F Igg From Various Sourcementioning

confidence: 99%

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Radioimmunometric Demonstration of Immunoglobulin G on Cancer Cells Derived from Malignant Pleural Effusions

Górny¹,

Micksche²,

Wolf³

1982

Oncology

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Well-preserved viable cancer cells, lymphocytes and mesothelial cells were prepared from various human malignant and non-malignant pleural effusions by a Ficoll-Hypaque discontinuous gradient. Using ¹²⁵I-labelled protein A from Staphylococcus aureus as tracer, IgG was found on all cancer cells and also on apparently non-malignant mesothelial cells. Loss of cell surface IgG by overnight incubation, and fluctuation of surface IgG levels in the same patient occurred with cancer cells as well as with mesothelial cells. The IgG in vivo coat of cancer cells reflected the IgG levels of the sera rather than those of the pleural effusions. Binding of IgG from autologous and allogeneic sera and from supernatantsof acid and mild overnight elution of cancer cells and mesothelial cells did not indicate a cancer-specific uptake of IgG. Various unspecific modes of IgG uptake by cancer cells are discussed including the possibility of a non-immune mechanism. Modification and loss of antigens by metastatic cells have also been considered.

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Section: '25i-s[)a Binding and Re-binding O F Igg From Various Sourcementioning

confidence: 99%

“…This technique has successfully been employed for the evaluation of xenoantisera to mel anoma-associated antigens [13,15).…”

Section: Introductionmentioning

confidence: 99%

Radioimmunometric Demonstration of Immunoglobulin G on Cancer Cells Derived from Malignant Pleural Effusions

Górny¹,

Micksche²,

Wolf³

1982

Oncology

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“…Attempts at serologic identification of antibodies to TAMA have employed cytotoxicity, complement-fixation, immunofluorescence, isotopic antiglobulin binding, as well as antibody-dependent cellular cytotoxicity (Ting and Herberman, 1976). Recent advances in SPA applications have resulted in the development of RIAs for quantitating soluble proteins (Langone, 1978) and normal (Welsch et al, 1975) as well as surface tumor cellular antigens (Brown et af., 1977;Zeltzer et al, 1977;Nayak et al, 1979). A modification of these latter methods employing glutaraldehyde-fixed mammalian tumor cells in suspension was developed by Rockoff et al (1979) and used for our work.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, the l2%SPA technique was modified for the detection of antibodies capable of binding to the surface of tumor cells grown in tissue culture (Brown et al, 1977;Zeltzer and Seeger, 1977). A recent report by Nayak et al, (1979) is similar in approach to these last methods except that the target cells are allowed to adhere to a microtiter plate and the individual wells must be separated by means of a band saw prior to quantification of radioactivity. A further modification of this protocal by Rockoff et al, (1979) employs glutaraldehydefixed tumor cells in suspension as a target for a solidphase RIA.…”

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confidence: 99%

Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma

Veltri

McKolanis

Rockoff

et al. 1980

Intl Journal of Cancer

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Xenoantisera were prepared in rabbits against intact paraformaldehyde-fixed Lewis lung carcinoma (LLCa) cells and membrane solubilized proteins extracted with Triton X-100 and 3M KCI. The antisera were analyzed for antibodies to tumor-associated membrane antigens (TAMA) by means of a solid-phase radioimmunoassay (RIA). THe immunoassay employed 125-labelled Staphylococcus aureus protein A (SPA) to detect specific antibody binding to glutaraldehyde-fixed Lewis lung tumor target cells. The protein-A antibody-binding (PAAB) method is rapid, sensitive and reproducible. The PAAB revealed a possible LLCa-TAMA as evidenced by the reactivity of xenoantisera raised to LL paraformaldehyde tumor cell vaccine (TCV) and LL tumor Triton X-100 (TTri) and 3M KCI (TKCI) extracts of membrane preparations. The reactivity of our antisera followed linear dose-response kinetics but the degree of reactivity decreased from anti-TCV to anti-TTri to anti-TKCI respectively. The LL-TAMA demonstrated specificity since our antisera did not significantly cross-react with normal C56BL/6 cellular antigens or other known murine tumor-associated surface antigens of virally and chemically-induced tumor systems.

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Activity of antibodies recovered from immune complexes of ovarian cancer patients

Lutz

Dawson

1984

Cancer Immunol Immunother

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Ascites fluids from ovarian cancer patients contain immune complexes (IC). Attempts were made to characterize those antigens to which the patient is reacting using antibody recovered from these complexes. Polyethylene glycol (PEG) precipitation and protein A Sepharose 4B affinity chromatography were used to isolate IC. Dissociation of the IC was achieved by G-150 gel filtration in 0.1 M acetic acid, 0.15 M NaCl. Activity of antibody preparations was measured using a binding assay with 125I-labeled staphylococcal protein A. Confluent monolayers of various cell lines (including human ovarian and cervical carcinoma lines, human lymphoblastoid cell lines, human and chick embryo fibroblasts) were used. Six of nine antibody preparations isolated from ovarian cancer patient IC contained at least some reactivity against all cell types. Specificity was further defined using cell adsorption assays and polyacrylamide gel electrophoresis. These results indicate what appears to be autoreactivity directed against a normal component(s) of many cells. No evidence for an ovarian cancer-restricted response was shown. Immunoprecipitation analyses showed several polypeptide bands on autoradiograms (49K, 46K, 33K, 25K), which deviated distinctly from the pattern obtained for whole cell extracts.

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Detection of antibodies bound to tumor cell surface antigens with radioiodinated Staphylococcus aureus protein A (SPA)

Cited by 8 publications

References 18 publications

Radioimmunometric Demonstration of Immunoglobulin G on Cancer Cells Derived from Malignant Pleural Effusions

Radioimmunometric Demonstration of Immunoglobulin G on Cancer Cells Derived from Malignant Pleural Effusions

Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma

Activity of antibodies recovered from immune complexes of ovarian cancer patients

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