Detection of antibodies in sera of weaned pigs after contact infection with Sarcoptes scabiei var. suis and after treatment with an antiparasitic agent by three different indirect ELISAs

Kessler, E; Matthes, H.-F.; Schein, E.; Wendt, Michael

doi:10.1016/s0304-4017(03)00098-0

Cited by 27 publications

(21 citation statements)

References 9 publications

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“…This is supported by Kessler et al (2003), (Tables 1, 2). The design of the study does not allow us to say whether there are any false positive results, but all of the sera were from a mangy farm and there were also no serological differences between the skin scraping positive and negative animals ( Table 2).…”

Section: Discussionmentioning

confidence: 65%

On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays

2004

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Two trials were carried out to compare the use of different enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against Sarcoptes scabiei var. suis. In the first trial, we employed four tests and 70 selected sera from a closed pig farm with sarcoptic mange. The sera were taken from the breeding area as well as from the finishing unit and compared with skin scrapings. The SARCOPTES-ELISA 2001 yielded the most positive results (88.58%), followed by the ELISA of the National Veterinary Institute, Uppsala (70%), the Acar-Test P-ELISA (52.86%), skin scrapings (48.57%), and the CHEKIT Sarcoptest (30%). In the second trial, eight litters from infected sows were examined from weeks 1-12 of life using the CHEKIT Sarcoptest and the SARCOPTES-ELISA 2001. The presence of maternal antibodies was highest on day 7 in both ELISAs and could be detected until weeks 5-9 of life. Antibodies increased as a result of an active immune response between days 56 and 63 in the SARCOPTES-ELISA 2001 and between days 70 and 77 of life in the CHEKIT Sarcoptest. Significant differences between the first and second litters were observed.

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Section: Discussionmentioning

confidence: 65%

On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays

2004

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“…In other animal models, the presence of asymptomatic seroreactors has been associated to the incubation phase [7], the subclinical course of the infestation [10] or the persistence of antibodies after therapy [23,25]. As regards ruminants, persistence of specific antibodies for more than two months after successful treatment with a macrocyclic lactone has been demonstrated in cattle [32].…”

Section: Discussionmentioning

confidence: 99%

Biotin-avidin amplified ELISA for detection of antibodies to Sarcoptes scabiei in chamois (Rupicapra spp.)

et al. 2004

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-Scabies is a major threat to the well being of mountain-dwelling Bovid hosts, Rupicapra rupicapra and Rupicapra pyrenaica. Severe outbreaks are in progress over a significant part of their distribution area and resource managers demand improved methods to monitor, analyse and possibly forecast the spread and effects of scabies at the population level. An amplified capture enzyme-linked immunosorbent assay was developed to detect antibodies to Sarcoptes scabiei in chamois (Rupicapra spp.) serum. The method used the biotin-avidin amplification system and was validated on a panel of 144 serum samples, of which 40 were obtained from scabietic and 104 from healthy unexposed individuals originating from a scabies-free area. The antigen, a whole body extract of the various developmental stages of S. scabiei, was prepared from mites actively leaving the skin lesions of naturally infested red foxes (Vulpes vulpes). The resulting LAB-ELISA was characterised by 93% sensitivity, 97% specificity and a high degree of repeatibility. A single seroreactor was found amongst 32 chamois affected with skin pathologies other than scabies, including infestations by other Acarina (Trombicula spp. and Ixodid ticks). Antibodies to S. scabiei were present in 26 out of 169 sera (15.4%) obtained by clinically healthy chamois within a scabies outbreak area, indicating that asymptomatic infestations by S. scabiei can be revealed by serological methods in the studied Caprinae hosts.Sarcoptes / chamois / ELISA / avidin-biotin

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“…The status of S. scabiei in pig herds is routinely monitored by serodiagnosis (Hejduk et al 2011). Kessler et al (2003) used indirect ELISA for detection of antibodies against S. scabiei var suis infested and treated pigs and opined that sensitivity of serological techniques must be taken into consideration for diagnosis particularly during its use in monitoring and eradication programs. For detection of antibodies in sera of S. scabiei infested pigs, Bornstein and Wallgren (1997) developed an ELISA where they observed the sensitivity and specificity of this serodiagnostic test is 87.8 and 99.5 %, respectively considering clinical diagnosis as gold standard.…”

Section: Diagnosismentioning

confidence: 99%

Sarcoptic mange infestation in pigs: an overview

Laha

2014

J Parasit Dis

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Sarcoptic mange infestation in pigs is caused by Sarcoptes scabiei var. suis. It is the most common mange infestation of pigs. The parasite is distributed worldwide. Pig owners are generally concerned about the internal parasitic infections and ignored the external parasitic infestations. But the external parasitic infestation with S. scabiei var. suis has economic significance as it causes morbidity, mortality, decreased fertility and feed conversion ratio in pigs. Keeping in view of importance of S. scabies var. suis infestation in pigs, this communication discussed about the present and past research works done on S. scabies var. suis infestation in pigs, particularly its prevalence, life cycle, pathological lesions, clinical symptoms, haematobiochemical changes, diagnosis, treatment and control, to have an idea about this infestation at a glance. It has been concluded that the research work done on sarcoptic mange infestation in pigs in India is less in comparison to other countries. It may be due to its consideration as a neglected parasite or due to it's under report. Organization of awareness programs for the farmers by extension personalities or other authorities might be able to save the farmers from economic losses due to this infestation.

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Detection of antibodies in sera of weaned pigs after contact infection with Sarcoptes scabiei var. suis and after treatment with an antiparasitic agent by three different indirect ELISAs

Cited by 27 publications

References 9 publications

On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays

On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays

Biotin-avidin amplified ELISA for detection of antibodies to Sarcoptes scabiei in chamois (Rupicapra spp.)

Sarcoptic mange infestation in pigs: an overview

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