Detection of antibodies specific for HLA-A,B,C,DR,DQ and DP by the erythrocyte antibody rosette inhibition (EAI) and immune phagocytosis inhibition (IPI) tests

Reduced Immune Phagocytosis of Monocytes from Neonates Whose Mothers Produce HLA Antibodies

“…The analysis of immune phagocytosis was carried out by a mod ification of the IPI test [1,2] omitting the inhibitory HLA antibody in vitro, and assuming its effect in vivo. In this way a 'direct IPI test' in analogy to the direct antiglobulin test was performed.…”

“…They were analyzed for HLA antibodies by the standard NIH microcyto toxicity test against T cells and -after absorption with pooled platelets -against B cells. In addition, the cytotoxicity test on adher ent monocytes [7] and the IPI test [2] were carried out employing a cell panel of selected donors ( Higher sensitization, e.g. using undiluted anti-D, results in higher IP: x±SD=59±7% [1].…”

“…IP inhibi tion (IPI) lasts for several days. The uptake of bacteria, fungi or foreign particles which does not depend on the Fc receptor is not inhibited [1][2][3].…”

“…We have developed a test procedure, the IPI test [1,2], which is positive in virtually all pregnancy sera which contain cytotoxic HLA ABC and DR antibodies [3]. In addition to these sera which are about 20-25% of all pregnancy sera, the IPI test detects those HLA antibodies which are not or only insufficiently cytotoxic.…”

Another Case of Transfusion-Related Lung Injury due to Anti-HNA-3a (-5b)

“…Granulocyte antibodies were investigated using the granulocyte immune fluorescence test (GIFT) [17] and the granulocyte aggregation test (GAT) [18]. HLA antibody screening was performed using the lymphocyte-cytotoxic test (LCT) [19] and the immune phagocytosis (Fcy receptor) inhibition test (IPI) [20]. Cell samples were obtained from 10 random donors to be used for antibody screening tests.…”