Detection of antibodies to Hypoderma lineatum in cattle by Western blotting with recombinant hypodermin C antigen

Boldbaatar, Damdinsuren; Xu, Xuan; Kimbita, Elikira; Huang, Xiaohong; Igarashi, Ikuo; Byambaa, Badarch; Battsetseg, Badgar; Battur, Banzragch; Battsetseg, G; Batsukh, Zayat; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi

doi:10.1016/s0304-4017(01)00457-5

Cited by 13 publications

(7 citation statements)

References 12 publications

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“…In Albania, the seropositivity rates were 38.7% and 41.3% in two sampling seasons (10). Our study found a higher rate of seroprevalence (73.8%) than that reported by some other researchers (10,13,14,17,19,30,33,34) and lower than that found in Mongolia by Boldbaatar et al (35). The seroprevalence rate ranged from 49.5% to 96.8% among the villages that were studied.…”

Section: Discussioncontrasting

confidence: 54%

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Epidemiological survey and economic significance of bovine hypodermosis on the Kars Plateau in the Northeast Anatolia Region of Turkey

Taşçi¹,

Sari²,

Aydın³

et al. 2018

Turk J Vet Anim Sci

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The aim of this study was to determine the prevalence and seroprevalence of hypodermosis and to calculate the economic loss associated with hide damage in the cattle on the Kars Plateau, Turkey. A total of 6563 randomly selected cattle were examined by skin palpation from 1 April to 31 May 2014. A subset of 465 serum samples was collected from October to November in 2014 and analyzed using a commercial ELISA kit. The overall prevalence of hypodermosis was 16.9% in the cattle examined by skin palpation. In respect of each of the risk factors assessed, the highest prevalence rates were recorded in 2-year-old (26.4%), Brown Swiss crossbred (17.9%), female (18.1%), and dark-colored (49.7%) cattle. A total of 15,349 warble larvae were detected under the skin of 1114 cattle. The larval count per animal ranged from 1 to 91, with a mean count of 13.8. Seroprevalence was found to be 73.8% (343/465), with the highest rates of infestation in 2-year-old (87.7%), dark-colored (81.1%), female (80.4%), and Brown Swiss crossbred (87.7%) cattle. The highest seroprevalence rate was 96.8% and the lowest was 49.5%. The economic loss caused by the disease was calculated as 18,615 Turkish lira in the survey areas.

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Section: Discussioncontrasting

confidence: 54%

“…The seroprevalence rates of hypodermosis were determined as 42.3% in Spain (34), 43.3% in Italy (30), between 6% and 51.7% in China (17), 96% in Mongolia, and 0% in Japan (35). In Albania, the seropositivity rates were 38.7% and 41.3% in two sampling seasons (10).…”

Section: Discussionmentioning

confidence: 99%

Epidemiological survey and economic significance of bovine hypodermosis on the Kars Plateau in the Northeast Anatolia Region of Turkey

Taşçi¹,

Sari²,

Aydın³

et al. 2018

Turk J Vet Anim Sci

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“…Sometime, western blotting technique is also used for the detection of the antibodies of H. lineatum. This technique is identical to that of ELISA (Boldbaatar et al 2001).…”

Section: Diagnosismentioning

confidence: 94%

Bovine hypodermosis—a global aspect

Hassan

Khan

Abubakar³

et al. 2010

Trop Anim Health Prod

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Cattle hypodermosis (warble fly infestation) is a notorious veterinary problem throughout the world. Larvae of Hypoderma species cause a subcutaneous myiasis of domesticated and wild ruminants. This disease is caused by, Hypoderma bovis, Hypoderma lineatum in cattle whereas, Hypoderma diana, Hypoderma actaeon, and Hypoderma tarandi, affect roe deer, red deer, and reindeer, respectively. Adults of the cattle grub are commonly known as heel flies, warble flies, bomb flies or gad flies. The biology of hypodermosis is complex because it passes through ecto- as well as endoparasitic stages in the life cycle. The parasitic stage of hypodermosis lasts about 1 year in domesticated as well as in the wild animals, while in the adult stage, a free-living fly lasts only for few days. The diagnosis of hypodermosis is of prime importance for planning treatment and the eradication program. Generally, there are two methods that are routinely used for diagnosis of hypodermosis, i.e., the direct clinical examination and immuno diagnosis by the use of pooled serum and/or milk sample. For the control of hypodermosis, different preparations are available and their use in most of the countries is limited to an individual level but never cover the whole cattle population of a country. Re-infestation in the herd occurs due to the untreated animals that remain the reservoir of the disease. The disease causes huge economic losses in animal production due to the effect of this disease on meat, milk, and the leather industry. It can also affect the general health status as well as the immune system of the body of the diseased animals. As regards the control measures of the disease, different methods have been efficiently practiced and consequently this disease is controlled at national level in many European countries.

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“…Proteins were electrophoretically transferred to polyvinylidene difluoride membranes (Millipore, Bedford, Mass. ), and then the membranes were treated as described previously (2).…”

Section: Methodsmentioning

confidence: 99%

Characterization ofToxoplasma gondiiSAG2 Expressed in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

Huang

Suzuki

et al. 2002

Clin Vaccine Immunol

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A baculovirus carrying the SAG2 gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs infected with T. gondii. Mice immunized with S-rSAG2 produced high titers of specific immunoglobulin G2a (IgG2a) and IgG1 antibodies. In an indirect fluorescent antibody test, all mouse antisera against S-rSAG2 reacted strongly to the natural parasites, but those against rSAG2 expressed in Escherichia coli (E-rSAG2) only showed very weak reaction, although no markedly difference was found in the reaction to denatured antigen, T. gondii lysate, in Western blot analysis. The results suggest that S-rSAG2 is better than E-rSAG2 in both antigenicity and immunogenicity. Enzyme-linked immunosorbent assay (ELISA) with S-rSAG2 could differentiate clearly between sera from 30 specific-pathogen-free cats and 4 experimentally infected cats. Serum samples from domestic cats in Japan were tested by the ELISA and compared with a latex agglutination test (LAT) and ELISA with E-rSAG2. Of 187 samples, all 35 LAT-positive sera had strong reactions to S-rSAG2 and E-rSAG2. Of the 152 LAT-negative sera, 18 were positive in the ELISA with S-rSAG2, whereas only 2 were positive in the ELISA with E-rSAG2. Although there were significant correlations among the three methods, the ELISA with S-rSAG2 was more sensitive than the others, which could be attributed to the fact that S-rSAG2 shares some common conformational structure with the native antigen. The results suggest that S-rSAG2 would be a useful reagent for the detection of T. gondii infection in cats.Toxoplasmosis is a worldwide zoonosis caused by Toxoplasma gondii. As many as one-third of the world's population shows serological evidence of infection (10). Although it is generally asymptomatic in healthy adults, it may cause abortion, neonatal death, severe sequelae in neonates, and lifethreatening lesions in AIDS patients. Furthermore, it may complicate immunosuppressive therapy. Of all the encephalitis occurring in human immunodeficiency virus-positive patients, ca. 50% is due to T. gondii infection (4). In addition to being as major source of infection for humans, it is also of considerable importance in domestic animals and is responsible for abortion in sheep and swine (25). Therefore, there is an urgent need to develop an effective diagnostic kit and vaccine.Surface antigen 2 (SAG2 and P22) of T. gondii is a major surface protein known as an attachment ligand (8) that also has good antigenicity and immunogenicity (1, 14, 21, 23). The recombinant SAG2 expressed in Escherichia coli (E-rSAG2) was effective in detecting the immunoglobulin G (IgG) antibody to T. gondii in human patients with acute toxoplasmosis (21, 23). In our previous study, E-rSAG2 was used as an antigen for ELISA to detect T. gondii infection in domestic cats and was shown to be a good reagent for the diagnosis of toxoplasmosis (9). Vaccination with E-rSAG2 provided partial protection against a lethal infection of T. g...

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Detection of antibodies to Hypoderma lineatum in cattle by Western blotting with recombinant hypodermin C antigen

Cited by 13 publications

References 12 publications

Epidemiological survey and economic significance of bovine hypodermosis on the Kars Plateau in the Northeast Anatolia Region of Turkey

Epidemiological survey and economic significance of bovine hypodermosis on the Kars Plateau in the Northeast Anatolia Region of Turkey

Bovine hypodermosis—a global aspect

Characterization ofToxoplasma gondiiSAG2 Expressed in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

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