Detection of antimicrobial resistance genes in urban air

Becsei, Ágnes; Solymosi, Norbert; Csabai, István; Magyar, Donát

doi:10.1002/mbo3.1248

Cited by 4 publications

(4 citation statements)

References 42 publications

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“…Analyzing the land use and weather conditions during the sampling period could potentially provide valuable insights and help identify explanatory variables. Indeed, previous studies have demonstrated that the urban environment, characterized by human activities, serves as a reservoir for AMR due to factors like water disposal systems and pollution [9,61,62]. Additionally, specific molecules, including cephalosporins (CTX, FOX, KF), tetracyclines (DO, TE), and penicillins (AX), were found to be statistically associated with provincial disparities.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial-Resistant Environmental Bacteria Isolated Using a Network of Honey Bee Colonies (Apis mellifera L. 1758)

Cilia,

Resci,

Scarpellini

et al. 2023

Transboundary and Emerging Diseases

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The phenomenon of antibiotic resistance stands as a paramount health challenge in the contemporary era. Within a One Health approach, it becomes crucial to effectively track the dissemination of antibiotic resistance, not only within humans and animals but also within the environment. To investigate the environment, the honey bee (Apis mellifera) has emerged as a prominent environmental bioindicator due to its social, behavioral, and morphological features. The objective of this study was to describe the antimicrobial resistance (AMR) patterns of bacterial isolates from the body surface and the gut of honey bees sampled from 33 colonies throughout the Emilia-Romagna region (Italy). A total of 608 strains were examined for 19 distinct antimicrobial compounds from various classes, and the results showed that more than 50% of the isolates for eight out of nine provinces showed characteristics of nonsusceptibility toward amoxicillin and penicillin, and, generally, 98.19% of isolated strains were considered AMR and 74.67% exhibited multidrug resistance (MDR) characteristics, more frequent in Gram-negative strains (87.74%) than in Gram-positive ones (60.34%). Additionally, a significant correlation with a lower prevalence of MDR bacteria was demonstrated for one province (Ferrara, odds ratio (OR) = 3.33, (1.67; 6.64), p = 0.0006 ). In conclusion, this study provides evidence for the utility of A. mellifera colonies as bioindicators for MDR bacteria, enabling their characterization and distribution at a geographical level. Additional investigations are required to further explore the potential role of honey bees as bioindicators for antimicrobial-resistant bacteria, particularly in terms of their association with environmental characteristics.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial-Resistant Environmental Bacteria Isolated Using a Network of Honey Bee Colonies (Apis mellifera L. 1758)

Cilia,

Resci,

Scarpellini

et al. 2023

Transboundary and Emerging Diseases

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“…The evidence has continuously shown that antimicrobial resistance has displayed its existence in various environments, such as human or animal hospitals [ 3 – 5 ], indigenous residents, wild animals [ 6 ], and water circumstances (including rivers, lakes, oceans, etc.) [ 7 , 8 ], soil [ 9 ], polar lands [ 10 ], food, sewage, sludge [ 11 , 12 ], and air [ 13 ]. While antimicrobial resistance genes (ARGs) have shared their specific ways to transfer via the horizontal genetic elements (HGEs), such as plasmids, integrons, transposons, and bacteriophages, among or between different species [ 14 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

The universality of eAREs in animal feces suggesting that eAREs function possibly in horizontal gene transfer

Jiang¹,

Zhao²,

Li³

et al. 2023

J Adv Vet Anim Res

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Objectives: This study aimed to pinpoint the universality of extracellular antimicrobial resistance elements (eAREs) and compare the contents of eAREs with those of intracellular AREs (iAREs) in animal feces, thus laying a foundation for the further analysis of the horizontal transfer of antimicrobial resistance genes (ARGs) in the animal guts. Materials and Methods: Extracellular DNAs were isolated from the fecal samples of Pavo cristatus (n = 18), Ursus thibetanus (n = 2), two breeds of broilers (n = 21 and 11, respectively), and from the contents of rabbit intestines (n = 5). eAREs were detected by PCR technology. iAREs in P. cristatus and broiler feces were also detected and compared with the corresponding eAREs. In addition, some gene cassettes of class 1 integrons were sequenced and analyzed. Results: The results showed that eAREs exist in animal feces and intestinal contents. In this study, different eAREs were detected from animal feces and intestinal contents, and tetA, tetB, sul1, sul2, class 1 integron, and IncFIB presented the highest detection rates. The detection rates of certain eAREs were significantly higher than those of parallel iAREs. The integral cassettes with intact structures were found in eAREs, and the cassettes carried ARGs. Conclusions: The presented study here sheds light on the presence of eAREs in animal feces or guts, and eAREs may play an important role in the horizontal gene transfer of ARGs.

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“…Traditionally in the extant literature, most airborne microorganism's characterization depended on culture-based methods [13][14][15], leading to an underestimation of the total bacterial diversity. The cultural method only recognizes 1.5-15.3% of all the species that are able to form a colony and the microorganisms that are not viable or unable to grow are not identified [16].…”

Section: Introductionmentioning

confidence: 99%

“…A dysbiosis state causes the reduction of immune-related commensal species and the increase of pathogens and pathobionts (commensal microorganisms which can become pathogens when genetic and environmental conditions change) [29,30]. Microorganisms that composed bioaerosol are also a reservoir for the resistome, the complex of the antimicrobial resistance genes (ARGs), further decreasing antibiotic's efficacy [15]. Exposure to PM-associated bioaerosol has been markedly associated with various health effects, mainly with infectious diseases but also respiratory diseases, allergies, and lung cancer [13,14,20,26,27,29].…”

Section: Introductionmentioning

confidence: 99%

Urban Aerobiome and Effects on Human Health: A Systematic Review and Missing Evidence

et al. 2022

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Urban air pollutants are a major public health concern and include biological matters which composes about 25% of the atmospheric aerosol particles. Airborne microorganisms were traditionally characterized by culture-based methods recognizing just 1.5–15.3% of the total bacterial diversity that was evaluable by genome signature in the air environment (aerobiome). Despite the large number of exposed people, urban aerobiomes are still weakly described even if recently advanced literature has been published. This paper aims to systematically review the state of knowledge on the urban aerobiome and human health effects. A total of 24 papers that used next generation sequencing (NGS) techniques for characterization and comprised a seasonal analysis have been included. A core of Proteobacteria, Actinobacteria, Firmicutes, and Bacteroides and various factors that influenced the community structure were detected. Heterogenic methods and results were reported, for both sampling and aerobiome diversity analysis, highlighting the necessity of in-depth and homogenized assessment thus reducing the risk of bias. The aerobiome can include threats for human health, such as pathogens and resistome spreading; however, its diversity seems to be protective for human health and reduced by high levels of air pollution. Evidence of the urban aerobiome effects on human health need to be filled up quickly for urban public health purposes.

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Detection of antimicrobial resistance genes in urban air

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 4 publications

References 42 publications

Antimicrobial-Resistant Environmental Bacteria Isolated Using a Network of Honey Bee Colonies (Apis mellifera L. 1758)

Antimicrobial-Resistant Environmental Bacteria Isolated Using a Network of Honey Bee Colonies (Apis mellifera L. 1758)

The universality of eAREs in animal feces suggesting that eAREs function possibly in horizontal gene transfer

Urban Aerobiome and Effects on Human Health: A Systematic Review and Missing Evidence

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