Detection of Apoptosis: From Bench Side to Clinical Practice

Bozza, William P.; Twomey, Julianne D.; Kim, Su-Ryun; Zhang, Baolin

doi:10.1007/978-1-4939-3588-8_2

Cited by 3 publications

(7 citation statements)

References 112 publications

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“…DAPI was used as a marker of DNA fragmentation seen in late apoptotic or necrotic cells. Mid-stage apoptosis was measured by caspase-3 activity, which plays a crucial role in coordinating the destruction of cellular structures, such as DNA fragmentation or degradation of cytoskeletal proteins. , …”

Section: Resultsmentioning

confidence: 99%

“…Mid-stage apoptosis was measured by caspase-3 activity, which plays a crucial role in coordinating the destruction of cellular structures, such as DNA fragmentation or degradation of cytoskeletal proteins. 79,80 From Figure 14b (the early-and late-stage apoptosis of the SCs on different scaffolds), the results indicate that the cells on the PLGA scaffolds have slightly lower relative populations in the early-and late-stage apoptosis than on the PLCL scaffolds. The scaffolds deposited by PPy (3D/E/PPy) promoted healthy cells and decreased both early-and late-stage apoptosis of the SCs when compared to the 3D and 3D/E scaffolds.…”

Section: Cell Proliferation and Attachmentmentioning

confidence: 99%

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Surface-Modified Polypyrrole-Coated PLCL and PLGA Nerve Guide Conduits Fabricated by 3D Printing and Electrospinning

et al. 2022

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The efficiency of nerve guide conduits (NGCs) in repairing peripheral nerve injury is not high enough yet to be a substitute for autografts and is still insufficient for clinical use. To improve this efficiency, 3D electrospun scaffolds (3D/E) of poly(l-lactide-co-ε-caprolactone) (PLCL) and poly(l-lactide-co-glycolide) (PLGA) were designed and fabricated by the combination of 3D printing and electrospinning techniques, resulting in an ideal porous architecture for NGCs. Polypyrrole (PPy) was deposited on PLCL and PLGA scaffolds to enhance biocompatibility for nerve recovery. The designed pore architecture of these “PLCL-3D/E” and “PLGA-3D/E” scaffolds exhibited a combination of nano- and microscale structures. The mean pore size of PLCL-3D/E and PLGA-3D/E scaffolds were 289 ± 79 and 287 ± 95 nm, respectively, which meets the required pore size for NGCs. Furthermore, the addition of PPy on the surfaces of both PLCL-3D/E (PLCL-3D/E/PPy) and PLGA-3D/E (PLGA-3D/E/PPy) led to an increase in their hydrophilicity, conductivity, and noncytotoxicity compared to noncoated PPy scaffolds. Both PLCL-3D/E/PPy and PLGA-3D/E/PPy showed conductivity maintained at 12.40 ± 0.12 and 10.50 ± 0.08 Scm–1 for up to 15 and 9 weeks, respectively, which are adequate for the electroconduction of neuron cells. Notably, the PLGA-3D/E/PPy scaffold showed superior cytocompatibility when compared with PLCL-3D/E/PPy, as evident via the viability assay, proliferation, and attachment of L929 and SC cells. Furthermore, analysis of cell health through membrane leakage and apoptotic indices showed that the 3D/E/PPy scaffolds displayed significant decreases in membrane leakage and reductions in necrotic tissue. Our finding suggests that these 3D/E/PPy scaffolds have a favorable design architecture and biocompatibility with potential for use in peripheral nerve regeneration applications.

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Section: Resultsmentioning

confidence: 99%

Section: Cell Proliferation and Attachmentmentioning

confidence: 99%

Surface-Modified Polypyrrole-Coated PLCL and PLGA Nerve Guide Conduits Fabricated by 3D Printing and Electrospinning

et al. 2022

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“…Later, DNA breaks were identified by finding that which caspases were activated. Currently used methods are based on morphological, immunohistochemical, immunological, biochemical, and molecular biology [13,[17][18][19][20]. These include light microscopy, special / fluorescent dyes, immunohistochemistry, electron microscopy, PCR, TUNEL, DNA agarose gel electrophoresis, Flow cytometry, In situ3 -end labeling method (ISEL), Western blotting and caspase colorimetric assay, Nuclease assay [20].…”

Section: Discussionmentioning

confidence: 99%

“…The biggest problem is that the methods used require separating, washing and transferring the cells. These procedures can damage cell membranes and alter the cell population distribution of viable, apoptotic and / or necrotic cells [13,[17][18][19][20].…”

Section: Discussionmentioning

confidence: 99%