Detection of apoptotic cells using a synthetic fluorescent sensor for membrane surfaces that contain phosphatidylserine

Koulov, Atanas V.; Stucker, Kenneth A.; Lakshmi, C.; Robinson, J. Paul; Smith, Bradley D.

doi:10.1038/sj.cdd.4401315

Cited by 98 publications

(154 citation statements)

References 7 publications

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“…[27] Another salient point is the difference in photophysics between the coordination complexes described here and PSS-380, our previously reported first-generation sensor for apoptosis. [20] Association of PSS-380 to a PS-rich membrane leads to fluorescence enhancement, due to enhanced binding of Zn 2 + to the DPA units in PSS-380, which decreases photoinduced electron transfer (PET) quenching. [28] In contrast, the PET-quenching pathway is not significant in PSS-480, and control studies with vesicles show that association of PSS-480 with PS-rich membranes does not alter its fluorescence intensity.…”

Section: Discussionmentioning

confidence: 99%

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Fluorescent Detection of Apoptotic Cells by Using Zinc Coordination Complexes with a Selective Affinity for Membrane Surfaces Enriched with Phosphatidylserine

et al. 2005

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The appearance of phosphatidylserine on the membrane surface of apoptotic cells (Jurkat, CHO, HeLa) is monitored by using a family of bis(Zn2+-2,2'-dipicolylamine) coordination compounds with appended fluorescein or biotin groups as reporter elements. The phosphatidylserine affinity group is also conjugated directly to a CdSe/CdS quantum dot to produce a probe suitable for prolonged observation without photobleaching. Apoptosis can be detected under a wide variety of conditions, including variations in temperature, incubation time, and binding media. Binding of each probe appears to be restricted to the cell membrane exterior, because no staining of organelles or internal membranes is observed.

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Section: Discussionmentioning

confidence: 99%

“…[20] Our initial studies used fluorophores with nonoptimal properties, [21] but the work produced important structural information that led us to the modular, three-component design shown in Scheme 1. The…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Detection of Apoptotic Cells by Using Zinc Coordination Complexes with a Selective Affinity for Membrane Surfaces Enriched with Phosphatidylserine

et al. 2005

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“…To confirm that Pah1p-GFP was indeed localized to the vacuolar membrane and not due to contaminating debris, we performed deconvolution fluorescence microscopy. Here, Pah1p-GFP vacuoles were co-stained with PSS-380 to label phosphatidylserine (57). Z-series of images were collected of individual vacuoles and deconvolved using Axiovision software.…”

Section: Ppah1mentioning

confidence: 99%

Yeast Lipin 1 Orthologue Pah1p Regulates Vacuole Homeostasis and Membrane Fusion

Sasser¹,

Qiu²,

Karunakaran³

et al. 2012

Journal of Biological Chemistry

144

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Background: Pah1p is a phosphatidic acid phosphatase that generates diacylglycerol. Results: Deletion of PAH1 alters the sorting of fusion factors to the vacuole and inhibits fusion. Conclusion: Conversion of phosphatidic acid to diacylglycerol is integral to vacuole homeostasis. Significance: This is the first report that links a Lipin 1 homologue to the recruitment and activation of a Rab GTPase or the sorting of SNARE proteins.

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“…This effect would be explained by their ability to sequester calcium from the phospholipid matrix, where it interacts with proteins of the coagulation cascade. Then it would form a protective cover that would isolate the apical surface of placental villi from circulating coagulation proteins [20]. It has been postulated that displacement of annexin V from the cellular surface by aannexin V Abs found in the sera of patients with APS, would be responsible for the generation of a thrombogenic environment and the consequent fetal loss [21].…”

Section: Introductionmentioning

confidence: 99%

Antiphospholipid and antioangiogenic activity in females with recurrent miscarriage and antiphospholipid syndrome

et al. 2016

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Ethical approval: The study was approved by the Bioethics Comitee of Facultad de Ciencias Bioquímicas y Farmacéuticas (Universidad Nacional de Rosario). Res Nº 076/2008. All participants signed the corresponding informed consent. Guarantor: SMMA Contributorship: HFP performed most assays and analysis of results, and wrote the paper. EP and CB performed some assays and collaborated in the analysis of results. JM performed patients selection for the study and aided in medical matters. MB, MJS and SMD performed sample collection and conditioning, as well as some laboratory determinations. HB performed statistical analysis. SMMA coordinated and supervised the whole study. Abstract word count: 251. Actual word count (excluding abstract and references): 2664 AbstractBackground: Antiphospholipid syndrome (APS) is an autoimmune disease characterized by thrombosis, fetal losses and thrombocytopenia associated to antiphospholipid (APL) antibodies (Abs). They are directed to phospholipids, such as cardiolipins (a-cardiolipin, ACA) and lupus anticoagulant (LA), or to complexes formed by phospholipids and protein cofactors, such as β2 glycoprotein 1 (a-β2GP1) and annexin V (a-annexin V). These auto Abs may be considered as a family of Abs involved in thrombotic events and APL activity. On the other hand, some proangiogenic factors are involved in the normal development of placental vasculature, such as the vascular endothelial growth factor (VEGF). Overexpression of VEGF receptor in its soluble form (sVEGFR-1) has been associated to a higher antiangiogenic activity. Our aim was to analyze the association between ACA, LA, a-β2GP1, a-annexin V and sVEGFR-1 with recurrent miscarriage before week 10 of gestation in women with APS. Methods: We studied 24 women (primary or secondary APS), who were divided into two groups: women with recurrent miscarriage before week 10 of gestation [M; n=12] and women with no history of fetal loss [NM; n=12]. ACA, a-β2GP1, aannexin V and sVEGF-R1 levels were assessed by ELISA, while LA was assessed by screening and confirmatory tests. Results: A significant association was observed between the number of positive biomarkers and the belonging group (p<0.05). Besides, a positive result for LA and a-β2GP1 was found to be significantly associated to the M group (p<0.05). Conclusions: LA and a-β2GP1 may be implicated in pregnancies complicated by recurrent miscarriage in women with APS.

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Detection of apoptotic cells using a synthetic fluorescent sensor for membrane surfaces that contain phosphatidylserine

Cited by 98 publications

References 7 publications

Fluorescent Detection of Apoptotic Cells by Using Zinc Coordination Complexes with a Selective Affinity for Membrane Surfaces Enriched with Phosphatidylserine

Fluorescent Detection of Apoptotic Cells by Using Zinc Coordination Complexes with a Selective Affinity for Membrane Surfaces Enriched with Phosphatidylserine

Yeast Lipin 1 Orthologue Pah1p Regulates Vacuole Homeostasis and Membrane Fusion

Antiphospholipid and antioangiogenic activity in females with recurrent miscarriage and antiphospholipid syndrome

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