Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study

Noto, A.M. Di; Sciortino, Sonia; Cardamone, Cinzia; Ciravolo, Cosimo; Napoli, Concetta; Alio, Vincenzina; Arculeo, Pietro; Oliveri, Giuseppa; Costa, Antonella

doi:10.4081/ijfs.2018.7171

Cited by 11 publications

(11 citation statements)

References 31 publications

(55 reference statements)

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“…Specifically, the results obtained in this study agree with previous studies, although with variable prevalence rates (González and Ferrús, 2011 ; Mottola et al ., 2016b ; González et al ., 2017 ; Ramees et al ., 2018 ; Vicente-Martins et al ., 2018 ; Kim et al ., 2019 ). However, the results of this study are in contrast with Di Noto et al ( 2018 ), who found vegetable samples negative for Arcobacter spp. The presence of Arcobacter spp.…”

Section: Discussioncontrasting

confidence: 99%

Occurrence and characterization of <em>Arcobacter</em> spp. from ready-to-eat vegetables produced in Southern Italy

et al. 2021

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Given that the number of foodborne illness outbreaks linked to the consumption of ready-to-eat vegetables has been widely documented and considering that data on the occurrence of Arcobacter spp. in such foodstuffs are lacking, the aim of the present study was to evaluate the presence of Arcobacter spp. and the occurrence of virulence factors as well as to genotype Arcobacter spp. in ready-to-eat (RTE) vegetable samples, using cultural and biomolecular assays. Arcobacter spp. was detected in 16/110 (14.5%) samples, with A. butzleri being detected in 15/16 and A. cryaerophilus in 1/16 isolates. PCRs aimed at the nine putative virulence genes demonstrated widespread distribution of such genes among A. butzleri and A. cryaerophilus isolates. In addition, multilocus sequence type (MLST) analysis revealed a low genetic diversity within the arcobacters isolates. The results underline the need to develop an appropriate surveillance system based on biomolecular characterization for an integrated microbiological risk assessment of ready-toeat vegetables, and consequently of composite foods.

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Section: Discussioncontrasting

confidence: 99%

Occurrence and characterization of <em>Arcobacter</em> spp. from ready-to-eat vegetables produced in Southern Italy

et al. 2021

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“…The reported prevalence of Arcobacter species in foods varies greatly among different studies. However, most studies agree that the contamination rates of poultry meat are higher in comparison to red meat, raw cow milk and vegetables [24,[39][40][41]. In this study, Arcobacter spp.…”

Section: Discussionsupporting

confidence: 60%

Prevalence, antimicrobial susceptibility and virulence gene profiles of Arcobacter species isolated from human stool samples, foods of animal origin, ready-to-eat salad mixes and environmental water

Uljanovas¹,

Gölz²,

Brückner³

et al. 2021

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Background Members of the genus Arcobacter are considered as emerging zoonotic food and waterborne pathogens that cause gastroenteritis and bacteremia in humans. However, the potential risk that Arcobacter species pose to public health remains unassessed in various countries, including Baltic states. Therefore, the aim of this study was to determine the prevalence, antimicrobial susceptibility and presence of putative virulence genes of Arcobacter isolates recovered from humans, food products and environmental water in Lithuania. Results A total of 1862 samples were collected and examined from 2018 to 2020 in the city of Kaunas. Overall, 11.2% (n = 208) of the samples were positive for the presence of Arcobacter spp. The highest prevalence was detected in chicken meat (36%), followed by environmental water (28.1%), raw cow milk (25%), ready-to-eat salad mixes (7.1%) and human stool (1.7%). A. butzleri was the most frequently isolated species (n = 192; 92.3%), followed by A. cryaerophilus (n = 16; 7.7%). Arcobacter spp. antimicrobial susceptibility testing revealed unimodally distributed aggregated minimal inhibitory concentrations (MICs) for gentamicin, tetracycline, ciprofloxacin, ampicillin and erythromycin. However, a bimodal distribution for azithromycin was found with 96.2% of determined MICs above the epidemiological cut-off value (ECOFF) defined for C. jejuni (0.25 µg/ml). Majority of the Arcobacter isolates (n = 187; 89.9%) showed high susceptibility to ciprofloxacin with MICs below or equal to the ECOFF value of 0.5 µg/ml. The putative virulence genes cadF (100%), ciaB (100%), cj1349 (99%), tlyA (99%), mviN (97.9%) and pldA (95.8%) were the predominant genes detected among A. butzleri isolates. In contrast, the mviN and ciaB genes were present in all, whereas cj1349 (12.5%), tlyA (25%) and hecA (12.5%) were only detected in few A. cryaerophilus isolates. Conclusions Our results demonstrate that food products and environmental water in Lithuania are frequently contaminated with Arcobacter spp. that carry multiple putative virulence genes. Furthermore, A. butzleri were isolated from 1.7% of inpatients. Fluoroquinolones and aminoglycosides were found to be more effective against Arcobacter in comparison to other antimicrobial agents. However, further studies are needed to determine the pathogenic mechanisms and factors that facilitate the spread of Arcobacter infections.

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“…DNA was extracted by using the protocol previously reported [ 15 ] and utilized as template in a multiplex PCR assay to amplify the 16S and 23S rRNA genes in order to obtain a specific and simultaneous identification of A. butzleri , A. cryaerophilus , and A. skirrowii [ 64 ]. The selected primers amplified a 401-bp fragment from A. butzleri , a 257-bp fragment from A. cryaerophilus , and a 641-bp fragment from A. skirrowii .…”

Section: Methodsmentioning

confidence: 99%

“…Arcobacter spp. are emerging entero-pathogens that can be isolated worldwide from different aquatic matrices, such as lakes and rivers [ 3 , 4 , 5 , 6 ], groundwater [ 7 , 8 ], wastewater [ 9 , 10 , 11 ], drinking water [ 12 ], seawater [ 13 , 14 ], and food of both animal and non-animal origin [ 15 , 16 , 17 , 18 ]. Thus, food and water are considered the main vehicle of the pathogen [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments

et al. 2021

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Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence and the antimicrobial resistance profile of Arcobacter isolates recovered from different aquatic sources. Besides, we searched for Arcobacter spp. in seaweeds and the corresponding seawater samples. Bacteriological and molecular methods applied to 100 samples led to the isolation of 28 Arcobacter isolates from 27 samples. The highest prevalence was detected in rivers followed by artificial ponds, streams, well waters, and spring waters. Seaweeds contained a higher percentage of Arcobacter than the corresponding seawater samples. The isolates were identified as Arcobacter butzleri (96.4%) and Arcobacter cryaerophilus (3.6%). All the isolates showed a multi-drug resistance profile, being resistant to at least three different classes of antibiotics. Molecular analysis of genetic determinants responsible for tetracycline resistance in nine randomly chosen isolates revealed the presence of tetO and/or tetW. This work confirms the occurrence and the continuous emergence of antibiotic-resistant Arcobacter strains in environmental samples; also, the presence of quinolone-resistant Arcobacter spp. in aquatic sources used for water supply and irrigation represents a potential risk for human health.

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Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study

Cited by 11 publications

References 31 publications

Occurrence and characterization of <em>Arcobacter</em> spp. from ready-to-eat vegetables produced in Southern Italy

Occurrence and characterization of <em>Arcobacter</em> spp. from ready-to-eat vegetables produced in Southern Italy

Prevalence, antimicrobial susceptibility and virulence gene profiles of Arcobacter species isolated from human stool samples, foods of animal origin, ready-to-eat salad mixes and environmental water

Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments

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