Detection of bacteria by 16S rRNA PCR and sequencing in culture‐negative chronic rhinosinusitis

Abstract

NA. Laryngoscope, 128:2223-2225, 2018.

Association of the sinonasal bacterial microbiome with clinical outcomes in chronic rhinosinusitis: a systematic review

“…The fourth and fifth columns of Table 2 lists the primers used by each study for polymerase chain reaction (PCR) amplification and 16s‐rRNA gene regions for bacterial identification. Each study used swabs as the means of sampling with some variability in the site used, while Karunasagar et al 28 . and Joss et al 29 .…”

“…reported SNOT‐22 scores for their patients but did not report any further analysis related to the microbiome and the survey results. However, Karunasagar et al 28 . showed that of the 9 patients with severe SNOT‐22 scores (>70), 5 had Staphylococcus species as their most abundant, 2 had Pseudomonas , 1 had Klebsiella , and 1 Escherichia .…”

“…Multiple authors failed to find a significant difference in bacterial composition between CRSwNP and CRSsNP 22,29 . Also of note, Karunsagar et al 28 . noted that in 2 patients with recurrent polyposis who were culture‐negative, Staphylococcus was detected in rRNA PCR, highlighting the importance of molecular techniques in this population.…”

From correlation to causality: the case of Subdoligranulum

“…The number of vials with visible growing within the three following days allowed the calculation of the live bacteria concentration in the culture. 41 For the administration to the mice, bacterial culture was centrifuged at 4000 g during 20 min at 4°C. Then supernatant was removed, and the pellet was washed twice in anoxic PBS supplemented with antioxidants and finally resuspended in glycerol (25% vol/vol) and stored at −80°C.…”

Composição e diversidade do microbioma do meato médio de pacientes com rinossinusite crônica com polipose nasal

“…Nas vias aéreas, o desbalanço do microbioma também se mostra um fator importante em doenças das vias inferiores, como em doenças obstrutivas pulmonares (60) . (61,62) . Estudos moleculares demonstraram a presença de microrganismos em seio esfenoidal, maxilar e etmoidal saudáveis (38,42) .…”