Detection of bacteria by time-resolved laser-induced breakdown spectroscopy

Morel, Stéphane; Leone, Nicolas; Adam, P.; Amouroux, J.

doi:10.1364/ao.42.006184

Cited by 190 publications

(100 citation statements)

References 11 publications

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“…As reported in LIBS studies on bacterial samples [38,39], the CN emission bands result either from the direct ablation of the C-N bonds of the amino acid structures that are part of living cells or from the recombination between C 2 from the plasma plume and N 2 from the ambient air. Independent from the mechanism of the CN-molecule formation, a higher intensity of the CN or C 2 bands indicates a higher content of the molecules and hence a higher concentration of carbon in the sample, as is the case for fat tissue in the present study.…”

Section: Resultsmentioning

confidence: 78%

Investigation of the differentiation ofex vivonerve and fat tissues using laser-induced breakdown spectroscopy (LIBS): Prospects for tissue-specific laser surgery

et al. 2016

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In the present study, the elemental compositions of fat and nerve tissue during their plasma mediated laser ablation are studied in the context of tissue differentiation for laser surgery applications by using Laser‐Induced Breakdown Spectroscopy (LIBS). Tissue samples of porcine fat and nerve were prepared as ex vivo experimental objects. Plasma mediated laser ablation is performed using an Nd : YAG laser in open air and under normal stray light conditions. The performed measurements suggest that the two tissue types show a high similarity in terms of qualitative elemental composition while at the same time revealing a distinct difference in the concentration of the constituent elements. Different analysis approaches are evaluated and discussed to optimize the tissue‐differentiation performance of the LIBS approach.

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Section: Resultsmentioning

confidence: 78%

Investigation of the differentiation ofex vivonerve and fat tissues using laser-induced breakdown spectroscopy (LIBS): Prospects for tissue-specific laser surgery

et al. 2016

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“…Breakdown spectroscopy: Many groups have also utilised different types of breakdown spectroscopy (BS) to identify elemental composition as means of biological aerosol detection and analysis, although these have not been as widely applied to ambient measurements. Laserinduced breakdown spectroscopy (LIBS) has easily been the most common form of BS method and has been employed to characterise pollen (Boyain-Goitia et al, 2003), and fungal spores (Hybl et al, 2003), bacteria (Morel et al, 2003) and a variety of biological aerosol particle types (Samuels et al, 2003). Other forms of elemental analysis such as spark-induced breakdown spectroscopy (SIBS), particle-induced X-ray emission (PIXE) and various forms of combustion analysis have also been utilised for laboratory study of biological aerosol (Sarantaridis and Caruana, 2010;Schmidt and Bauer, 2010).…”

Section: Light Detection and Ranging (Lidar) And Remote Sensingmentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,194

1,114

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A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.

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“…Morel et al 301 used the LIBS technique for analyzing biological matter for the detection of biological hazards. Eight species were considered in this experiment, 6 bacteria and 2 pollens in pellet form.…”

Section: Military and Homeland-security Applicationsmentioning

confidence: 99%

Laser Induced Breakdown Spectroscopy

Pasquini¹,

Cortez²,

Silva³

et al. 2007

J. Braz. Chem. Soc.

342

195

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Esta revisão descreve os aspectos fundamentais, a instrumentação, as aplicações e tendências futuras de uma técnica analítica que se encontra em seu estágio de consolidação e que está em vias de estabelecer o seu nicho entre as técnicas espectrofotométricas modernas. A técnica é denominada Espectroscopia de Emissão em Plasma Induzido por Laser (em inglês, Laser Induced Breakdown Spectroscopy, LIBS) e sua principal característica está no uso de pulsos de laser como fonte de energia para vaporizar a amostra e excitar a emissão de radiação eletromagnética, a partir de seus elementos e/ou fragmentos moleculares. A radiação emitida é analisada por meio de instrumentos ópticos de alta resolução e as suas intensidades são medidas, usualmente com detectores rápidos de estado sólido. Em conjunto, esses dispositivos permitem a geração e a medida de um espectro de emissão de faixa ampla do fenômeno induzido pelo pulso de laser. O espectro registrado contém informação qualitativa e quantitativa que pode ser correlacionada com a identidade da amostra ou empregada na determinação da quantidade de seus constituintes. Essa revisão é dividida em quatro partes. A primeira aborda aspectos históricos da técnica e os conceitos teóricos relevantes associados com LIBS; então, os aspectos práticos de diversas abordagens experimentais e instrumentais empregadas na implementação da técnica são revistos de forma crítica; as aplicações encontradas na literatura, incluindo aquelas que empregam quimiometria, são classificadas e exemplificadas por meio de trabalhos relevantes recentemente publicados. Finalmente, uma tentativa de estabelecer uma avaliação global e as perspectivas futuras para a técnica é apresentada.This review describes the fundamentals, instrumentation, applications and future trends of an analytical technique that is in its early stages of consolidation and is establishing its definitive niches among modern spectrometric techniques. The technique has been named Laser Induced Breakdown Spectroscopy (LIBS) and its main characteristic stands in the use of short laser pulses as the energy source to vaporize samples and excite the emission of electromagnetic radiation from its elements and/or molecular fragments. The emitted radiation is analyzed by high resolution optics and the intensities are recorded, usually by fast triggered solid state detectors. Together, these devices allow producing and registering a wide ranging emission spectrum of the short-lived phenomenon induced by the laser pulse. The spectrum contains qualitative and quantitative information which can be correlated with sample identity or can be used to determine the amount of its constituents. This review is divided in four parts. First, the relevant historical and theoretical concepts associated with LIBS are presented; then the main practical aspects of the several experimental and instrumental approaches employed for implementation of the technique are critically described; the applications related in the literature, including those making use of chemometri...

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Detection of bacteria by time-resolved laser-induced breakdown spectroscopy

Cited by 190 publications

References 11 publications

Investigation of the differentiation ofex vivonerve and fat tissues using laser-induced breakdown spectroscopy (LIBS): Prospects for tissue-specific laser surgery

Investigation of the differentiation ofex vivonerve and fat tissues using laser-induced breakdown spectroscopy (LIBS): Prospects for tissue-specific laser surgery

Primary biological aerosol particles in the atmosphere: a review

Laser Induced Breakdown Spectroscopy

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