Detection of Bacterial Alkaline Phosphatase Activity by Enzymatic In Situ Self-Assembly of the AIEgen-Peptide Conjugate

Zhang, Xue; Ren, Chunhua; Hu, Fang; Gao, Yang; Wang, Zhongyan; Li, Huiqiang; Liu, Jianfeng; Liu, Bin; Yang, Cuihong

doi:10.1021/acs.analchem.9b05704

Cited by 85 publications

(47 citation statements)

References 51 publications

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“…The supernatant was taken and the OD value at 540 nm was measured by microplate reader (Thermo Fisher, HYX-YQ0015, USA). The hemolysis rate was calculated with reference to the following formula [ 41 ].

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Section: Methodsmentioning

confidence: 99%

Water-soluble PANI:PSS designed for spontaneous non-disruptive membrane penetration and direct intracellular photothermal damage on bacteria

Tang

Liu

et al. 2021

Bioactive Materials

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The major challenge in the field of antibacterial agents is to overcome the low-permeability of bacteria cell membranes that protects the cells against diverse drugs. In this work, water-soluble polyaniline (PANI)-poly (p-styrenesulfonic acid) (PSS) (PANI:PSS) is found to spontaneously penetrate bacteria cellular membranes in a non-disruptive way, leaving no evidence of membrane poration/disturbance or cell death, thus avoiding side effects caused by cationic ammonia groups in traditional ammonia-containing antibacterial agents. For aqueous synthesis, which is important for biocompatibility, the polymer is synthesized via an enzyme-mimetic route relying on the catalysis of a nanozyme. Owing to its fluorescent properties, the localization of as-prepared PANI:PSS is determined by the confocal microscope, and the results confirm its rapid entry into bacteria. Under 808 nm near-infrared (NIR) irradiation, the internalized PANI:PSS generates local hyperthermia and destroys bacteria highly efficiently from inside the cells due to its excellent photothermal effects. Staphylococcus aureus ( S. aureus ), M ethicillin-resistant Staphylococcus aureus ( MRSA ) and Escherichia coli ( E. coli ) could be effectively eliminated as well as the corresponding bacterial biofilms. Results of in vivo antibacterial experiments demonstrate excellent antibacterial activities of the water-soluble PANI:PSS without side effects. Therefore, the prepared water-soluble polymer in this study has great potential in the treatment of various bacterial infections.

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…”

Section: Methodsmentioning

confidence: 99%

Water-soluble PANI:PSS designed for spontaneous non-disruptive membrane penetration and direct intracellular photothermal damage on bacteria

Tang

Liu

et al. 2021

Bioactive Materials

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“…In addition, they offer a complementary strategy to smart wound technologies and point of care (PoC) devices [11] . Current small‐molecule fluorescent probes utilise enzyme‐based biomarkers to facilitate the detection of pathogenic bacteria, [12] which include elastases, [10e] phosphatases, [13] glycosidases, [14] proteases [15] and lipases [16]…”

Section: Methodsmentioning

confidence: 99%

The Evaluation of Ester Functionalised TCF‐Based Fluorescent Probes for the Detection of Bacterial Species

Gwynne

Williams

Yan

et al. 2021

Israel Journal of Chemistry

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The ester functionality is commonly seen in the areas of chemical biology and medicinal chemistry for the design of cell‐permeable active molecules. Ester‐based pro‐drug/pro‐sensor strategies are employed to mask polar functional groups (i. e. carboxylic acids) and improve the overall cell permeability of these functional molecules. However, their use as reactive units for sensing applications, including bacterial detection, has not been fully explored. Herein, we synthesised two TCF‐based fluorescent probes, TCF‐OAc and TCF‐OBu. As expected, both TCF‐OAc and TCF‐OBu demonstrated a significant fluorescence (22‐ and 43‐fold, respectively) and colorimetric response (yellow to purple) towards porcine liver esterase (PLE) with a limit of detection of 1.18 mU/mL and 0.45 mU/mL, respectively. With these results in hand, the ability of these probes to detect planktonic suspensions of gram‐positive Staphylococcus aureus (S. aureus) and gram‐negative Pseudomonas aeruginosa (P. aeruginosa), and Escherichia coli (E. coli) were evaluated. Different fluorescence responses for gram‐positive and gram‐negative bacteria were observed between TCF‐OAc and TCF‐OBu. After 1 h incubation, TCF‐OAc proved more sensitive towards S. aureus, demonstrating a significant fluorescence “turn on” response (16‐fold); whereas, TCF‐OBu was more selective towards P. aeruginosa, with a 22‐fold increase in the fluorescence response observed. These results demonstrate the influence of the ester chain length on the selectivity for bacterial species.

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“…In another study, Yang et al developed an alkaline phosphatase (ALP)-activated fluorescent turn-on AIEgen-peptide conjugate probe 24 (Figure 12A) (Zhang et al, 2020b). Probe 24 can undergo dephosphorylation by bacterial ALP to form an aggregated fibrous structure on the bacterial surface and provide AIE-based fluorescence.…”

Section: Alkaline Phosphatase Detectionmentioning

confidence: 99%

Strategies of Detecting Bacteria Using Fluorescence-Based Dyes

et al. 2021

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Identification of bacterial strains is critical for the theranostics of bacterial infections and the development of antibiotics. Many organic fluorescent probes have been developed to overcome the limitations of conventional detection methods. These probes can detect bacteria with “off-on” fluorescence change, which enables the real-time imaging and quantitative analysis of bacteria in vitro and in vivo. In this review, we outline recent advances in the development of fluorescence-based dyes capable of detecting bacteria. Detection strategies are described, including specific interactions with bacterial cell wall components, bacterial and intracellular enzyme reactions, and peptidoglycan synthesis reactions. These include theranostic probes that allow simultaneous bacterial detection and photodynamic antimicrobial effects. Some examples of other miscellaneous detections in bacteria have also been described. In addition, this review demonstrates the validation of these fluorescent probes using a variety of biological models such as gram-negative and -positive bacteria, antibiotic-resistant bacteria, infected cancer cells, tumor-bearing, and infected mice. Prospects for future research are outlined by presenting the importance of effective in vitro and in vivo detection of bacteria and development of antimicrobial agents.

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Detection of Bacterial Alkaline Phosphatase Activity by Enzymatic In Situ Self-Assembly of the AIEgen-Peptide Conjugate

Cited by 85 publications

References 51 publications

Water-soluble PANI:PSS designed for spontaneous non-disruptive membrane penetration and direct intracellular photothermal damage on bacteria

Water-soluble PANI:PSS designed for spontaneous non-disruptive membrane penetration and direct intracellular photothermal damage on bacteria

The Evaluation of Ester Functionalised TCF‐Based Fluorescent Probes for the Detection of Bacterial Species

Strategies of Detecting Bacteria Using Fluorescence-Based Dyes

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