Detection of Bacterial Deposits and Bio Aerosols by Time-Resolved Laser-Induced Breakdown Spectroscopy (Trelibs)

Leone, Nicolas; D'Arthur, G.; Adam, P.; Amouroux, J.

doi:10.1615/hightempmatproc.v8.i1.10

Cited by 10 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Introduction Laser-induced breakdown spectroscopy (LIBS) is a sensitive spectrochemical technique that has recently begun to be used for characterizing biological samples such as microorganisms (i.e., bacteria) and tissues [1][2][3][4][5][6][7][8][9][10][11][12]. The speed, portability, and robustness of the technique suggest that LIBS may be applicable as rapid point-of-care medical diagnostic technology.…”

mentioning

confidence: 99%

Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: the effect of mixed cultures and sample dilution on bacterial identification

2010

View full text Add to dashboard Cite

Rehse, Steven J.; Mohaidat, Q.I.; and Palchaudhuri, S.. (2010). Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: The effect of mixed cultures and sample dilution on bacterial identification. Laser-induced breakdown spectroscopy has been utilized to classify and identify bacterial specimens on the basis of their atomic composition. We have characterized the effect that the presence of a second bacterial species in the ablated specimen had on the identification of the majority species. Specimens with a reduced number of bacterial cells (approximately 2500) were identified with 100% accuracy when compared to undiluted specimens. In addition, a linear dependence of the total spectral power as a function of cell number was determined. Lastly, a high selectivity was obtained for a LIBS-based analysis of nine separate bacterial strains from four genera.

show abstract

mentioning

confidence: 99%

Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: the effect of mixed cultures and sample dilution on bacterial identification

2010

View full text Add to dashboard Cite

show abstract

“…These authors tested six different biotypes of bacteria, include three bacilli (spore forming) bacteria, and species of Escherichia, Staphylococcus, and Proteus. This group expanded upon this work by developing an aerosol delivery system to illustrate the feasibility of using LIBS to detect biological aerosols [40]. The demonstration of the detection of single cells (1-5 m) in aerosol form had been demonstrated for the first time a year earlier by Hybl et al in dense clouds and streams [30].…”

Section: Initial Studiesmentioning

confidence: 99%

“…In some of the early experiments previously described, it was initially believed that a univariate analysis of the intensities or ratios of intensities of certain lines in the LIBS spectrum (e.g. Ca or Mg) could provide sufficient information to discriminate the bacteria [28,39,40,43,44]. Other groups utilized modified linear correlation techniques [42] comparing the relative intensities of one or several elements [48].…”

Section: Chemometricsmentioning

confidence: 99%

A review of the use of laser-induced breakdown spectroscopy for bacterial classification, quantification, and identification

Rehse

2019

Spectrochimica Acta Part B: Atomic Spectroscopy

View full text Add to dashboard Cite

The use of laser-induced breakdown spectroscopy to determine the elemental composition of bacterial cells has been described in the peer-reviewed literature since 2003. Fifteen years on, significant accomplishments have been reported that have served to clarify and underscore the areas of bacteriological investigation that LIBS is well-suited for as well as the challenges that yet remain to be faced. This review will attempt to summarize the state of the field by surveying the available body of knowledge. The early days of these experiments, roughly from 2003 to 2007, in which many of the most fundamental experiments were initially conducted will be described. The more in-depth investigations that followed in the subsequent decade will then be detailed. Many important aspects of performing LIBS on bacterial cells were reported on and are summarized here including: the use of chemometric algorithms for statistical classification of unknown spectra; the influence of the mounting substrate on classification; the effect of the testing gas atmosphere and the choice of bacterial cell growth nutrient medium on the measured LIBS spectrum; the efficacy of a LIBS-based test as a genus-level or strainlevel discrimination test; the ability of LIBS to determine the cell titer or concentration of cells in the initial sample; the effects that possible contaminations or interferents within the sample would have on the LIBS spectrum; the influence that environmental stresses the cells may be exposed to during growth and the state of reproductive health of the cells could have on the LIBS spectrum; the use of standoff or remote apparatus to minimize the risk to the operators during bacteriological identification of unknown specimens; and the combination of other optical modalities with LIBS to enhance the sensitivity or specificity of identification. Lastly, tables are provided which summarize both every species of bacteria ever tested with LIBS as well as the major lessons learned by the community through 15 years of careful investigation. laser-induced breakdown spectroscopy; pathogens; bacteria

show abstract

“…Specifically, it must be proven that the ability to identify a given target organism is not eliminated by the bacteria's exposure to a wide variety of growth conditions and/or nutrient environments. Early work by Leone et al 27 concluded that discrimination of two strains of Bacillus globigii cultured in two different kinds of nutritive media was indeed possible. We have also demonstrated that P. aeruginosa cultured in a trypticase soy agar ͑TSA͒ medium ͑a surrogate for a sputum sample͒ and on a blood agar plate ͑BAP͒ ͑a surrogate for a blood sample͒ showed no substantial difference in our discrimination.…”

Section: A Laser-induced Breakdown Spectroscopy On Biological Samplesmentioning

confidence: 99%

A membrane basis for bacterial identification and discrimination using laser-induced breakdown spectroscopy

Rehse

Jeyasingham

Diedrich

et al. 2009

Journal of Applied Physics

View full text Add to dashboard Cite

Follow this and additional works at: https://scholar.uwindsor.ca/physicspub Recommended Citation Recommended Citation Rehse, Steven J.; Jeyasingham, N.; and Palchaudhuri, S.. (2009). A membrane basis for bacterial identification and discrimination using laser-induced breakdown spectroscopy. Journal of Applied Physics, 105 (10).Nanosecond single-pulse laser-induced breakdown spectroscopy ͑LIBS͒ has been used to discriminate between two different genera of Gram-negative bacteria and between several strains of the Escherichia coli bacterium based on the relative concentration of trace inorganic elements in the bacteria. Of particular importance in all such studies to date has been the role of divalent cations, specifically Ca 2+ and Mg 2+ , which are present in the membranes of Gram-negative bacteria and act to aggregate the highly polar lipopolysaccharide molecules. We have demonstrated that the source of emission from Ca and Mg atoms observed in LIBS plasmas from bacteria is at least partially located at the outer membrane by intentionally altering membrane biochemistry and correlating these changes with the observed changes in the LIBS spectra. The definitive assignment of some fraction of the LIBS emission to the outer membrane composition establishes a potential serological, or surface-antigen, basis for the laser-based identification. E. coli and Pseudomonas aeruginosa were cultured in three nutrient media: trypticase soy agar as a control, a MacConkey agar with a 0.01% concentration of bile salts including sodium deoxycholate, and a trypticase soy agar with a 0.4% deoxycholate concentration. The higher concentration of deoxycholate is known to disrupt bacterial outer membrane integrity and was expected to induce changes in the observed LIBS spectra. Altered LIBS emission was observed for bacteria cultured in this 0.4% medium and laser ablated in an all-argon environment. These spectra evidenced a reduced calcium emission and in the case of one species, a reduced magnesium emission. Culturing on the lower ͑0.01%͒ concentration of bile salts altered the LIBS spectra for both the P. aeruginosa and two strains of E. coli in a highly reproducible way, although not nearly as significantly as culturing in the higher concentration of deoxycholate did. This was possibly due to the accumulation of divalent cations around the bacteria by the formation of an extracellular polysaccharide capsule. Lastly, a discriminant function analysis demonstrated that in spite of alterations in the LIBS spectrum induced by growth in the three different media, the analysis could correctly identify all samples better than 90% of the time. This encouraging result illustrates the potential utility of LIBS as a rapid bacteriological identification technology.

show abstract

Detection of Bacterial Deposits and Bio Aerosols by Time-Resolved Laser-Induced Breakdown Spectroscopy (Trelibs)

Cited by 10 publications

References 0 publications

Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: the effect of mixed cultures and sample dilution on bacterial identification

Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: the effect of mixed cultures and sample dilution on bacterial identification

A review of the use of laser-induced breakdown spectroscopy for bacterial classification, quantification, and identification

A membrane basis for bacterial identification and discrimination using laser-induced breakdown spectroscopy

Contact Info

Product

Resources

About