Detection of bacterial growth in blood components using oxygen consumption as a surrogate marker in a tertiary oncology setup

Chavan, P. D.; Bhat, VG; Ojha, Shashank; Rs, Kelkar; Rajadhyaksha, SB; Marathe, AN

doi:10.4103/0255-0857.96695

Cited by 5 publications

(1 citation statement)

References 11 publications

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“…Much effort has thus been devoted to the rapid and sensitive detection of bacterial pathogens in blood 18 – 21 . The enhanced bacterial detection (eBDS) system (Haemonetics Corporation, Braintree, MA, USA) indirectly detects bacteria by measuring decreases in oxygen concentration over 24 h, but cannot detect anaerobic bacteria 22 . The Platelet Pan-Genera Detection (PGD) test (Verax Biomedical, Marlborough, MA, USA) is a lateral flow immunoassay detecting lipoteichoic acid (LTA) and lipopolysaccharide (LPS) in aerobic and anaerobic Gram-positive and -negative bacterial species, respectively, within 30 min 23 .…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of bacterial contamination in plasma using magnetic-based enrichment

Lee

Abafogi

et al. 2022

Sci Rep

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Bacterial contamination of blood products is a major problem in transfusion medicine, in terms of both morbidity and mortality. Platelets (PLTs) are stored at room temperature (under constant agitation) for more than 5 days, and bacteria can thus grow significantly from a low level to high titers. However, conventional methods like blood culture and lateral flow assay have disadvantages such as long detection time, low sensitivity, and the need for a large volume of blood components. We used real-time polymerase chain reaction (PCR) assays with antibiotic-conjugated magnetic nanobeads (MNBs) to detect enriched Gram-positive and -negative bacteria. The MNBs were coated with polyethylene glycol (PEG) to prevent aggregation by blood components. Over 80% of all bacteria were captured by the MNBs, and the levels of detection were 101 colony forming unit [CFU]/mL and 102 CFU/mL for Gram-positive and -negative bacteria, respectively. The detection time is < 3 h using only small volumes of blood components. Thus, compared to conventional methods, real-time PCR using MNBs allows for rapid detection with high sensitivity using only a small volume of blood components.

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Section: Introductionmentioning

confidence: 99%

Molecular detection of bacterial contamination in plasma using magnetic-based enrichment

Lee

Abafogi

et al. 2022

Sci Rep

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Prévention du risque bactérien : inactivation des pathogènes/détection des bactéries

Morel

Naegelen

Deschaseaux

et al. 2013

Transfusion Clinique et Biologique

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Detection of bacterial contamination in platelet concentrates from Brazilian donors by molecular amplification of the ribosomal 16S gene

Viana

Ferreira

Matana

et al. 2018

Transfusion Medicine

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Objective:The aim of our work was to establish a semi-automated high-throughput DNA amplification method for the universal screening of bacteria in platelet concentrates (PCs).Background: Among cases of transfusion transmission of infectious agents, bacterial contamination ranks first in the number of events, morbidity and mortality. Transmission occurs mainly by transfused PCs. Automated culture is adopted by some blood banks for screening of bacterial contamination, but this procedure is expensive and has a relatively long turnaround time.Methods: PCs were spiked with suspensions of five different bacterial species in a final concentration of 1 and 10 colony-forming units (CFU) per millilitre. After incubation, the presence of bacteria was investigated by real-time polymerase chain reaction (PCR) and by the Enhanced Bacterial Detection System (eBDS, Pall) assay as a reference method. Real-time PCR amplification was performed with a set of universal primers and probes targeting the 16S rRNA gene. Co-amplification of human mitochondrial DNA served as an internal control. Results:Using the real-time PCR method, it was possible to detect the presence of all bacterial species tested with an initial concentration of 10 CFU mL −1 24 h after contamination, except for Staphylococcus hominis. The PCR assay also detected, at 24 h, the presence of Serratia marcescens and Enterobacter cloacae with an initial concentration of 1 CFU mL −1 . Conclusions:The real-time PCR assay may be a reliable alternative to conventional culture methods in the screening of bacterial

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Detection of bacterial growth in blood components using oxygen consumption as a surrogate marker in a tertiary oncology setup

Cited by 5 publications

References 11 publications

Molecular detection of bacterial contamination in plasma using magnetic-based enrichment

Molecular detection of bacterial contamination in plasma using magnetic-based enrichment

Prévention du risque bactérien : inactivation des pathogènes/détection des bactéries

Detection of bacterial contamination in platelet concentrates from Brazilian donors by molecular amplification of the ribosomal 16S gene

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