Detection of Bartonella sp. and a novel spotted fever group Rickettsia sp. in Neotropical fleas of wild rodents (Cricetidae) from Southern Brazil

Schott, Diogo; Umeno, Karen; Dall’Agnol, Bruno; Souza, Ugo Araújo; Webster, Anelise; Michel, Thais; Peters, Felipe Bortolotto; Christoff, Alexandre Uarth; André, Marcos Rogério; Ott, Ricardo; Jardim, Márcia M. A.; Reck, José

doi:10.1016/j.cimid.2020.101568

Cited by 12 publications

(10 citation statements)

References 37 publications

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“…Indeed, gltA sequences from B. harrusi sp. nov. showed 100% identity with Bartonella genotypes previously detected in rodent-associated fleas (Polygenis (Polygenis) bohlsi bolshi, Polygenis occidentalis occidentalis, Polygenis platensis and Craneopsylla minerva minerva) in the biomes of Pantanal (the state of Mato Grosso do Sul), Pampa and the Atlantic Forest, in the state of Rio Grande do Sul [16,68]. Interestingly, the Bartonella genotypes detected in fleas from centralwestern and southern Brazil were found in regions where the circulation of B. machadoae, a species recently described in rodents, was also reported [10].…”

Section: Discussionmentioning

confidence: 61%

First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella

et al. 2022

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The genus Bartonella (Rhizobiales: Bartonellaceae) encompasses facultative intracellular Gram-negative alphaproteobacteria that parasitize mainly erythrocytes and endothelial cells, as well as macrophages, monocytes and dendritic cells. Although they can infect numerous mammal species and arthropod vectors worldwide, reports of Bartonella infections in marsupials are scarce. In fact, such agents have only been detected in marsupials and/or associated ectoparasites in Australia and the United States of America until the present moment. The present study aimed to isolate and characterize molecularly, morphologically and phenotypically Bartonella infecting free-living marsupials sampled in the Brazilian Pantanal, the largest wetland in South America. Two marsupials were captured in December 2018 and six marsupials in February 2019, totaling eight small mammals sampled: five (62.5%) Thylamys macrurus and three (37.5%) Monodelphis domestica. All blood samples were submitted to qPCR for Bartonella spp. based on the nuoG gene, a pre-enrichment liquid culture and a chocolate agar solid culture. Bartonella sp. was isolated from 3 T. macrurus and one M. domestica. One Bartonella isolate obtained from a T. macrurus blood sample (strain 117A) that showed to be closely related to the Bartonella vinsonii complex and Bartonella machadoae was selected for whole genome sequencing using a hybrid approach based on Illumina NovaSeq and Nanopore sequencing platforms. This strain showed a genome of 2.35 Mbp, with an average C + G content of 38.8%, coding for 2013 genes, and a 29 kb plasmid with an average C + G content of 34.5%. In addition, this strain exhibited an average nucleotide identity (ANI) of 85% with Bartonella species belonging to the B. vinsonii group and 91% with B. machadoae. Phylogenomic analysis based on 291 protein coding genes shared by the genomes of 53 Bartonella species positioned this strain closely to B. machadoae. This new isolated species was named Bartonella harrusi sp. nov., which was characterized as having small capnophilic, microaerophilic and aerobic rods with an absence of pili and flagella. In conclusion, the present work describes the biochemical, phenotypic and genomic characteristics of Bartonella harrusi, a new species isolated from the T. macrurus blood samples of the Brazilian Pantanal. Finally, a review of the taxonomic classification of members of the genus Bartonella is proposed, based on the ANI values accessed by whole genome sequencing analyses.

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Section: Discussionmentioning

confidence: 61%

First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella

et al. 2022

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“…The zoonotic potential is yet unknown, and further studies should be carried out, since Bartonella species belonging to B. vinsonii complex are associated with clinical manifestations in humans in different regions of the world 37 . Fleas have been suggested as putative vector for Bartonellla among rodents in Brazil 65 , 66 . Indeed, Bartonella sp.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, Bartonella sp. has been molecularly detected in Polygenis bohlsi bolshi fleas collected from rodents in the Pantanal wetlands biome, Mato Grosso do Sul state, central-western Brazil 65 , as well as in Polygenis occidentalis, Polygenis platensis , and Craneopsylla minerva from Pampa grasslands and Atlantic rainforest biomes, in the State of Rio Grande do Sul, southern Brazil 66 . Recently, B. machadoae has been molecularly detected in Amblyomma sculptum nymphs collected from wild boars ( Sus scrofa ), which showed to be negative for Bartonella spp.…”

Section: Discussionmentioning

confidence: 99%

Co-infection by multiple vector-borne agents in wild ring-tailed coatis (Nasua nasua) from Iguaçu National Park, southern Brazil

Perles

Moraes

Silva

et al. 2023

Sci Rep

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The present study aimed to detect molecularly the presence of co-infections by vector-borne agents (VBA) in ring-tailed coatis’ (Nasua nasua) blood samples from Iguaçu National Park (INP), southern Brazil, and assess the phylogenetic positioning of the detected agents. DNA blood samples were submitted to molecular screening and characterization for Anaplasmataceae agents, Piroplasmids, Hepatozoon sp., hemotropic mycoplasmas, and Bartonella spp. In total, 42 (85.7%) coatis were positive for hemotropic Mycoplasma sp., 12 (24.5%) for Bartonella machadoae, 7 (14.3%) for Anaplasma sp. closely related to ‘Candidatus Anaplasma brasiliensis’, and 3 (6%) for Hepatozoon procyonis. The most prevalent co-infections observed was from bacterial VBA: while 18.3% were co-infected by hemotropic Mycoplasma sp. and Bartonella sp., 12.2% were co-infected by Anaplasma sp. and hemotropic Mycoplasma sp. Only two animals (4%) presented co-infections by three VBA (Bartonella sp., Anaplasma sp. and hemotropic Mycoplasma sp.). The coati is a wild carnivore found in INP, mainly in areas visited by tourists. These animals are frequently seen searching for food in garbage dumps or in tourists’ belongings. The present study expands the host specificity range of B. machadoae, which has been isolated only from rodents until the present moment. Since the zoonotic potential and transmission routes of the detected VBA are not yet known, surveillance in this area is much needed.

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“…The mechanisms of pathogen transmission by fleas are often multifactorial, and each species has several transmission routes to ensure maintenance. Therefore, it is essential to conduct further experimental studies on the capacity of different species of fleas to transmit rickettsial bacteria, considering their wide distribution in the fleas of the Atlantic Forest ecoregion (Horta et al, 2007;Schott et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Ecology of fleas and their hosts in the trifinio of north‐east Argentina: first detection of Rickettsia asembonensis in Ctenocephalides felis felis in Argentina

Urdapilleta

Pech-May

Lamattina

et al. 2021

Medical Vet Entomology

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Fleas are important in public health due to their role as parasites and vectors of pathogens, including Rickettsia. The aim of this study was to evaluate the diversity, abundance and prevalence of fleas and the presence of Rickettsia in the trifinio of north-east Argentina. Fleas from household and synanthropic animals were obtained from urban and periurban areas. They were taxonomically identified and samples of 227 fleas in 86 pools were analysed by polymerase chain reaction targeting the gltA and ompB genes of Rickettsia spp. The study revealed that Ctenocephalides felis felis was dominant on dogs, cats and opossums, with higher prevalence in the periurban area. The Shannon-Wiener and Morisita-Horn indices expressed differences in the diversity and similarity values of the absolute abundances of the species between the areas compared. DNA amplifications revealed 30.8% C. f . felis pools positive for Rickettsia spp. Phylogenetic analysis showed that the haplotype obtained was identical to Rickettsia asembonensis from Peru and Brazil. This is the first detection in Argentina of R. asembonensis that infects C. f . felis, and we emphasize the importance of conducting research from a 'One Health' perspective on the role of opossums and rodents in the integration of the transmission cycles of rickettsial bacteria.

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Detection of Bartonella sp. and a novel spotted fever group Rickettsia sp. in Neotropical fleas of wild rodents (Cricetidae) from Southern Brazil

Cited by 12 publications

References 37 publications

First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella

First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella

Co-infection by multiple vector-borne agents in wild ring-tailed coatis (Nasua nasua) from Iguaçu National Park, southern Brazil

Ecology of fleas and their hosts in the trifinio of north‐east Argentina: first detection of Rickettsia asembonensis in Ctenocephalides felis felis in Argentina

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