Detection of biofilm formation of a collection of fifty strains of Staphylococcus aureus isolated in Algeria at the University Hospital of Tlemcen

Abstract

The burden of disease caused by Staphylococcus aureus continues to grow; this organism has the ability to form biofilm and it is also a frequent cause of medical device and implant-related infections. The objective of this study was to evaluate the biofilm-forming ability of a collection of clinical isolates of S. aureus. In a total of 240 Staphylococcus spp. isolated from catheters, retrieved at five services (neonatology, internal medicine, pneumology, pediatric and neurology), only 50 (20.83%) strains were identified by conventional microbiological methods as S. aureus species; these strains were screened by microtiter plate assay for detection of biofilm formation. Of the 50 clinical isolates, 16 (32%) were non adherent, 20(40%) weakly, 10 (20%) moderately and 4(8%) strongly adherent. The quantitative method of microtiter plate can be involved as a simple, rapid, inexpensive and reproducible assay to assess biofilm formation which is further an important feature of pathogenecity of S. aureus in the clinical setting.

In-Vitro Biofilm Formation and Antimicrobial Resistance of Escherichia coli in Diabetic and Nondiabetic Patients

“…[21, 26]. It has been reported that more than 65% of all bacterial infections are related to biofilm production; however, it also depends on the type of the infection and etiology [27]. Previous studies have also highlighted the role of biofilm formation in UTI [28, 29].…”

Molecular Detection of Biofilm-Producing <i>Staphylococcus aureus</i> Isolates from National Orthopaedic Hospital Dala, Kano State, Nigeria

“…Diabetic foot wound patients with S. aureus colonization have a 2-fold increase in healing time [17]. Biofilm protection of microorganisms from opsonophagocytosis and antibiotics make S. aureus capable of causing chronic infection and sepsis an economically important organism in nosocomial infections [18].…”

Investigation of Biofilm Formation Among Methicillin-Resistant Staphylococcus aureus Isolated from Children

“…3 U n c o r r e c t e d P r o o f (36.7%) strains produced no biofilm. Other studies have reported a slightly higher number of biofilm producers among Staphylococcal species (23,29). In the CRA method, biofilm formation was observed in 80 (81.6%) isolates whereas 17 (17.3%) strains did not show any biofilm formation.…”

“…The OD cut-off value was separately calculated for each Microtiter plate. Biofilm formation by isolates was calculated and categorized according to the absorbance of the crystal violetstained attached cells (23,24) (Table 2).…”