Detection of biofilm related genes, classical enterotoxin genes and agr typing among Staphylococcus aureus isolated from bovine with subclinical mastitis in southwest of Iran

Khoramrooz, Seyed Sajjad; Mansouri, Fariba; Marashifard, Masoud; Hosseini, Seyed Ali; Chenarestane-Olia, Fereshteh Akbarian; Ganavehei, Banafsheh; Gharibpour, Farzaneh; Shahbazi, Afsane; Mirzaii, Mehdi; Darban-Sarokhalil, Davood

doi:10.1016/j.micpath.2016.05.022

Cited by 44 publications

(41 citation statements)

References 55 publications

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“…High frequency of MRSA in Iran in nosocomial and community-acquired infections causes many concerns about increasing rate of these organisms in the country. As we expected, there was no resistance to the effective drugs such as tigecycline, linezolid, synercid (quinupristin/dalfopristin), teicoplanin, and vancomycin that are in agreement with other studies from Iran [2,13]. In contrast with some studies [18,19], we found high rate of resistance to rifampicin (53%) in MRSA isolates that is in agreement with other studies [2,20].…”

Section: Discussionsupporting

confidence: 92%

“…SCCmec type III has been found to be the main type in other studies from Iran with varying ranges from 45% to 76% [11,21,23,24]. Although some studies from the east of Asia including Taiwan, Thailand, Vietnam, India, Sri Lanka, China, and Hong Kong reported SCCmec types II and IVc as the main types, SCCmec type III has been found to be the most isolated type in hospital-acquired infections in this area [13]. We found six different spa types among MRSA strains.…”

Section: Discussionmentioning

confidence: 77%

“…Boiling method was used for DNA extraction of S. aureus colonies [4,13]. For confirmation of S. aureus, PCR amplification of S. aureus-specific nuclease (nucA) gene was performed as described previously [14].…”

Section: Dna Extraction and Identification Of Mrsa Isolatesmentioning

confidence: 99%

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High frequency of methicillin-resistant Staphylococcus aureus (MRSA) with SCCmec type III and spa type t030 in Karaj’s teaching hospitals, Iran

Bayat

Zade

Mansouri

et al. 2017

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Methicillin-resistant Staphylococcus aureus (MRSA) has been one of the most important antibiotic-resistant pathogen in many parts of the world over the past decades. This cross-sectional study was conducted to investigate MRSA isolated between July 2013 and July 2014 in Karaj, Iran. All tested isolates were collected in teaching hospitals from personnel, patients, and surfaces and each MRSA was analyzed by SCCmec and spa typing. Antibiotic susceptibility testing was accomplished by disk diffusion method. Out of 49 MRSA isolates from the Karaj's teaching hospitals, 82%, 10%, and 6% of the isolates were SCCmec types III, II, and I, respectively. The main spa type in this study was spa t030 with frequency as high as 75.5% from intensive care unit (ICU) of the hospitals and high rate of resistance to rifampicin (53%) was found in MRSA isolates. In conclusion, high frequency of spa t030 with SCCmec type III and MRSA phenotype illustrated circulating of one of the antibiotic-resistant strains in ICU of Karaj's teaching hospitals and emphasizes the need for ongoing molecular surveillance, antibiotic susceptibility monitoring, and infection control.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 77%

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High frequency of methicillin-resistant Staphylococcus aureus (MRSA) with SCCmec type III and spa type t030 in Karaj’s teaching hospitals, Iran

Bayat

Zade

Mansouri

et al. 2017

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“…In this study agr type III was the most predominant agr type (71.9%) among S. aureus isolates, which is in accordance with a previous report from Brazil (48.2%) (Silva et al, 2013 ). However, agr I and agr II could be predominant types according to previous reports (Fabres-Klein et al, 2015 ; Khoramrooz et al, 2016 ; Mistry et al, 2016 ). Only 14.6 and 13.5% of our isolates were identified as agr I and IV respectively, which are lower than previous reports (Fabres-Klein et al, 2015 ; Mistry et al, 2016 ).…”

Section: Discussionmentioning

confidence: 62%

Prevalence and Characterization of Staphylococcus aureus Cultured From Raw Milk Taken From Dairy Cows With Mastitis in Beijing, China

et al. 2018

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The colonization of dairy herds and subsequent contamination of raw milk by Staphylococcus aureus (S. aureus), especially those expressing a multi-drug resistance (MDR), biofilm and toxins producing ability, remains an important issue for both the dairy producer and public health. In this study, we investigated the prevalence, antimicrobial resistance, virulence, and genetic diversity of S. aureus in raw milk taken from 2 dairy farms in Beijing, China. Ninety (46.2%, 90/195) samples were positive for S. aureus. Resistant to penicillin (PEN) (31.3%), ciprofloxacin (18.8%) and enrofloxacin (15.6%) were the most often observed. Isolates cultured from farm B showed significantly higher resistance to penicillin (73.9%), ciprofloxacin (34.8%), enrofloxacin (34.8%), tilmicosin (17.4%), and erythromycin (17.4%) than those from farm A (p < 0.05). Totally, 94.8% S. aureus harbored at least one virulence gene and the pvl (93.8%), sec (65.6%), and sea (60.4%) genes were the most frequently detected. The pvl and sec genes were more often detected in isolates from farm A (97.3% and 84.9% respectively) than those from farm B (p < 0.05). Of all 77 staphylococcus enterotoxin (SE)-positive isolates, more than 90% could produce enterotoxins and 70.1% could produce two types. Biofilm related genes (icaA/D, clf/B, can, and fnbA) were detected in all96 isolates. All 96 isolates could produce biofilm with 8.3, 70.8, and 18.8% of the isolates demonstrating weak, moderate and strong biofilm formation, respectively. A total of 5 STs, 7 spa types (1 novel spa type t17182), 3agr types (no agrII), and 14 SmaI-pulso-types were found in this study. PFGE cluster II-CC1-ST1-t127-agr III was the most prevalent clone (56.3%). Isolates of agr III (PFGE Cluster I/II-CC1-ST1-t127/2279) had higher detection of virulence genes than those of agr I and agr IV. TheMSSA-ST398-t1456-agr I clone expressed the greatest MDRbut with no virulence genes and weakly biofilm formation. Our finding indicated a relatively high prevalence of S. aureus with less antimicrobial resistance but often positive for enterotoxigenicity and biofilm formation. This study could help identify predominant clones and provide surveillance measures to eliminate and decrease the contamination of S. aureus in raw milk of dairy cows with mastitis.

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“… Cafiso et al (2007) also reported that agr group II S. aureus strains are usually prolific biofilm formers, while strains in agr group III are less so. However, Khoramrooz et al (2016) reported a significant association between agr group III and biofilm production in S. aureus isolates, and concluded that the type III isolates are potent biofilm producers. The relationship between S. aureus agr groups and antibiotic resistance is also of interest.…”

Section: Association Of Agr Types With Specific Bimentioning

confidence: 99%

Therapeutic Targeting of the Staphylococcus aureus Accessory Gene Regulator (agr) System

et al. 2018

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Staphylococcus aureus can cause numerous different diseases, which has been attributed to its large repertoire of virulence factors, many of which are under the control of the accessory gene regulator (agr) quorum sensing system. Under conditions of high cell density, agr increases the production of many virulence factors, decreases expression of several colonization factors, and is intimately associated with the pathogenesis and biofilm formation of S. aureus. This review summarizes our current understanding of the molecular mechanisms underlying agr quorum sensing and the regulation of agr expression. The discussion also examines subgroups of agr and their association with different diseases, and concludes with an analysis of strategies for designing drugs and vaccines that target agr to combat S. aureus infections.

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Detection of biofilm related genes, classical enterotoxin genes and agr typing among Staphylococcus aureus isolated from bovine with subclinical mastitis in southwest of Iran

Cited by 44 publications

References 55 publications

High frequency of methicillin-resistant Staphylococcus aureus (MRSA) with SCCmec type III and spa type t030 in Karaj’s teaching hospitals, Iran

High frequency of methicillin-resistant Staphylococcus aureus (MRSA) with SCCmec type III and spa type t030 in Karaj’s teaching hospitals, Iran

Prevalence and Characterization of Staphylococcus aureus Cultured From Raw Milk Taken From Dairy Cows With Mastitis in Beijing, China

Therapeutic Targeting of the Staphylococcus aureus Accessory Gene Regulator (agr) System

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