Detection of biological responses to low-dose radiation in humans

Yamaguchi, Masaru; Tatara, Yota; Nugraha, Eka Djatnika; Ramadhani, Dwi; Tamakuma, Yuki; Sato, Yoshiaki; Miura, Tomisato; Hosoda, Masahiro; Yoshinaga, Shinji; Syaifudin, Mukh; Kashiwakura, Ikuo; Tokonami, Shinji

doi:10.1016/j.freeradbiomed.2022.04.006

Cited by 6 publications

(15 citation statements)

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“…Based on the results of our previous report [ 1 ], the oxidative modification sites of MSA obtained in this study were compared with the results of humans with chronic low-dose radiation exposure ( Figure 5 ). Among the identified amino acid sequences of mouse albumin, lysine, methionine and tyrosine underwent dose-dependent oxidative modification.…”

Section: Resultsmentioning

confidence: 98%

“…In our previous study, we developed an MRM-HR method targeting the 38 patterns of hOMSA using LS-MS/MS and performed the assay on serum samples collected from the residents of a newly discovered HBRA (annual effective dose approximately 50 mSv y −1 ) [ 1 ]. As a result, we reported a dose-dependent oxidative change in a specific sequence of human serum albumin.…”

Section: Discussionmentioning

confidence: 99%

“…The measurement was performed according to a previous report [ 1 ]. Briefly, serum proteins were precipitated with acetone and the precipitates were dissolved and denatured with 50% trifluoroethanol.…”

Section: Methodsmentioning

confidence: 99%

“… Comparison of oxidative modification sequences of human and mouse serum albumin. Based on the results of our previous report [ 1 ], the oxidative modification sites of the serum albumin obtained in this study were compared. Among the identified amino acid sequences of mouse albumin, lysine, methionine and tyrosine underwent dose-dependent oxidative modification.…”

Section: Figurementioning

confidence: 99%

“…Very recently we reported on a proteomic analysis and oxidative modification profiling of serum collected from residents of a newly discovered high-level natural background radiation area (HBRA, annual effective dose of approximately 50 mSv y −1 ) and normal-level background radiation area (NBRA, 1.22 mSv y −1 ) in Mamuju, Indonesia [ 1 ]. A proteomic analysis showed that the apolipoprotein B-100 and hemoglobin subunit α1 expression of residents in the HBRA was significantly lower than that of residents in the NBRA.…”

Section: Introductionmentioning

confidence: 99%

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Serum Proteomic and Oxidative Modification Profiling in Mice Exposed to Total Body X-Irradiation

et al. 2022

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The details of the dose-dependent response of serum proteins exposed to ionizing radiation, especially the oxidative modification response in amino acid sequences of albumin, the most abundant protein, are unknown. Thus, a proteomic analysis of the serum components from mice exposed to total body X-irradiation (TBI) ranging from 0.5 Gy to 3.0 Gy was conducted using LC-MS/MS. The analysis of oxidative modification sequences of albumin (mOMSA) in TBI mouse serum revealed significant moderate or strong correlations between the X-irradiation exposure dose and modification of 11 mOMSAs (especially the 97th, 267th and 499th lysine residues, 159th methionine residue and 287th tyrosine residues). In the case of X-irradiation of serum alone, significant correlations were also found in the 14 mOMSAs. In addition, a dose-dependent variation in six proteins (Angiotensinogen, Odorant-binding protein 1a, Serine protease inhibitor A3K, Serum paraoxonase/arylesterase 1, Prothrombin and Epidermal growth factor receptor) was detected in the serum of mice exposed to TBI. These findings suggest the possibility that the protein variation and serum albumin oxidative modification responses found in exposed individuals are important indicators for considering the effects of radiation on living organisms, along with DNA damage, and suggests their possible application as biomarkers of radiation dose estimation.

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Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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