Detection of biological thiols based on a colorimetric method

Xu, Yuanyuan; Sun, Yangyang; Zhang, Yujuan; Lu, Chenhe; Miao, Jinfeng

doi:10.1631/jzus.b1500232

Cited by 11 publications

(7 citation statements)

References 21 publications

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“…Molecular-Dimension-Mediated Ultrasensitive and Fast-Responsive Fluorosensing of Biothiols. Given amino acids are considered as potential biomarkers and include immense biological relevance, 22,54 we perused their fluorosensing studies counting on noteworthy optical emission and N-functionality-engineered optimum-sized pores in this MOF. Fluorescence titrations were executed via sequential addition of 1 mM aqueous solution of different amino acids, having assorted functional groups, to 18a (Figures S20−S26).…”

Section: ■ Results and Discussionsupporting

confidence: 60%

Brønsted Acid-Functionalized Ionic Co(II) Framework: A Tailored Vessel for Electrocatalytic Oxygen Evolution and Size-Exclusive Optical Speciation of Biothiols

Goswami

Karthick

Das

et al. 2022

ACS Appl. Mater. Interfaces

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Metal−organic frameworks (MOFs) not only combine globally demanded renewable energy generation and environmental remediation onto a single platform but also rationalize structure−performance synergies to devise smarter materials with remarkable performance. The robust and non-interpenetrated cationic MOF exemplifies a unique bifunctional scaffold for the efficient electrochemical oxygen evolution reaction (OER) and ultrasensitive monitoring of biohazards. The microporous framework containing Brønsted acidfunctionalized [Co 2 (μ 2 -OH)(CO 2 ) 2 ] secondary building units (SBUs) exhibits remarkable OER performance in 1 M KOH, requiring 410 mV overpotential to obtain 10 mA cm −2 anodic current density, and a low Tafel slope of 55 mV/dec with 93.1% Faradaic efficiency. Apart from the high turnover frequency and electrochemically assessable surface area, steady OER performance over 500 cycles under potentiodynamic and potentiostatic conditions result in long-term catalyst durability. The highly emissive attribute from nitrogen-rich fluorescent struts benefits the MOF in recyclable and selective fluoro-detection of three biothiols (L-cysteine, homocysteine, and glutathione) in water with a fast response time. In addition to colorimetric monitoring in the solid and solution phases, control experiments validate size-exclusive biothiol speciation through molecular-dimension-mediated pore diffusion. The role of SBUs in the OER mechanism is detailed from density functional theory-derived free energy analysis, which also validates the importance of accessible N-sites in sensing via portraying framework−analyte supramolecular interactions.

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Section: ■ Results and Discussionsupporting

confidence: 60%

Brønsted Acid-Functionalized Ionic Co(II) Framework: A Tailored Vessel for Electrocatalytic Oxygen Evolution and Size-Exclusive Optical Speciation of Biothiols

Goswami

Karthick

Das

et al. 2022

ACS Appl. Mater. Interfaces

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“…In the presence of biothiols, the Ag-S bond is preferred; thus, the DNA binding protection of AgNPs is cancelled and the salt-induced aggregation is possible, which results in the color change from yellow to brown. Indirect colorimetric method was presented in the work of Xu et al [113]. The determination is based on 3,3′,5,5′tetramethylbenzidine (TMB) causing a reduction of Ag + , which results in a formation of AgNPs and a blue color of the solution.…”

Section: Colorimetrymentioning

confidence: 99%

Use of metal nanoparticles for preconcentration and analysis of biological thiols

2022

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Metal nanoparticles (NPs) exhibit several unique physicochemical properties, including redox activity, surface plasmon resonance, ability to quench fluorescence, biocompatibility, or a high surface‐to‐volume ratio. They are being increasingly used in analysis and preconcentration of thiol containing compounds, because they are able to spontaneously form a stable Au/Ag/Cu–S dative bond. They thus find wide application in environmental and particularly in medical science, especially in the analysis of biological thiols, the endogenous compounds that play a significant role in many biological systems. In this review article, we provide an overview of various types of NPs that have been applied in analysis and preconcentration of biological thiols, mainly in human biological fluids. We first discuss shortly the types of NPs and their synthesis, properties, and their ability to interact with thiol compounds. Then we outline the sample preconcentration and analysis methods that were used for this purpose with special emphasis on optical, electrochemical, and separation techniques.

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“…High level of biothiols can cause serious diseases such as cardiovascular diseases, Alzheimer and Parkinson, while low level of biothiols can cause liver damage, slowed growth and weight loss. They found that the presence of biothiols can change the color of test solution from blue to colorless [6]. Muhammad Zahir et al have proved that color change of tropical fruits can be related to its intrinsic properties such as soluble solid content and pH.…”

Section: 𝐴 = ∈ 𝑙𝑐 (1)mentioning

confidence: 99%

Specialized Colorimeter for Phenol red pH Measurement

Louis

Hamid

Raffay

et al. 2022

J. Phys.: Conf. Ser.

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The phenol red is commonly used in cell culture medium as visual pH indicator by changing its color from darker red color to the orange-yellowish color as the pH is changing from neutral to acidic level. The color change of phenol red is hardly to be determined by using naked eyes that will be interpreted differently by different person. Thus, this study is executed to develop a specialized colorimeter for phenol red pH measurement by using Raspberry Pi as a microcontroller unit, green LED as a light source and photodiode in tandem with capacitor as a light sensor. The Raspberry Pi calculated the capacitor charging time which is in relative to the amount of light sensed by the photodiode. Based on the results, the capacitor charging time can be related to the pH value by using non-linear relationship with high accuracy of R2 = 0.8946 and RMSE = 0.4447. This study successfully introduced the development of low-cost system for phenol red pH measurement by applying the capacitor charging time which is in correspond with the absorbance value.

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Detection of biological thiols based on a colorimetric method

Cited by 11 publications

References 21 publications

Brønsted Acid-Functionalized Ionic Co(II) Framework: A Tailored Vessel for Electrocatalytic Oxygen Evolution and Size-Exclusive Optical Speciation of Biothiols

Brønsted Acid-Functionalized Ionic Co(II) Framework: A Tailored Vessel for Electrocatalytic Oxygen Evolution and Size-Exclusive Optical Speciation of Biothiols

Use of metal nanoparticles for preconcentration and analysis of biological thiols

Specialized Colorimeter for Phenol red pH Measurement

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