Detection of blaOXA-145, blaOXA-224, blaOXA-539, and blaOXA-675 Genes and Carbapenem-Hydrolyzing Class D β-Lactamases (CHDLs) in Clinical Isolates of Pseudomonas aeruginosa Collected from West of Iran, Hamadan

Sezadehghani, Arash; Dehbashi, Sanaz; Tahmasebi, Hamed; Arabestani, Mohammad Reza

doi:10.1155/2022/3841161

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…bla SHV is recognized as the most prevalent ESBL gene that confers antibiotic resistance to pathogenic bacteria worldwide, primarily in E. coli and Klebsiella spp. [28][29][30][31], whereas bla OXA is typically identified in Pseudomonas aeruginosa [32] and Acinetobacter baumannii [33]. However, R. ornithinolytica harboring bla SHV and bla OXA has been isolated from a patient in China [34].…”

Section: Discussionmentioning

confidence: 99%

Emergence of NDM-1-producing Raoultella ornithinolytica from reservoir water in Northeast Thailand

Karnmongkol,

Wiriyaampaiwong,

Teerakul

et al. 2023

Vet World

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Background and Aim: Antibiotic resistance is a major global health threat. The increasing prevalence of drug-resistant bacteria poses a serious challenge to the effective treatment of infections in both humans and animals. Water is a major source of human and animal exposure to bacteria, and the presence of drug-resistant bacteria in water could present a severe threat to public health and animal production. This study investigated the presence of drug-resistant bacteria in Lam Pao Dam (LPD) water in Kalasin, Thailand. Materials and Methods: Ampicillin-resistant strains were obtained from LPD water and identified using 16s rDNA sequencing. Antibiotic resistance genes were detected by polymerase chain reaction using specific primers. The presence of antibiotic-resistant bacteria was evaluated using 16s amplicon analysis. The minimum inhibitory concentration (MIC) of Raoultella ornithinolytica strains against antibiotics was determined. Results: A total of 12 R. ornithinolytica, 4 Bacillus cereus, and 4 Enterococcus faecalis isolates were resistant to ampicillin. Almost all R. ornithinolytica strains harbored blaSHV and blaOXA genes, and two strains also harbored the blaNDM-1 gene. All four E. faecalis strains harbored the blaIMP gene. The most abundant species in the LPD sample was Exiguobacterium indicum, followed by E. faecalis and R. ornithinolytica. The MICs of 10 R. ornithinolytica strains against five antibiotics revealed that all strains were resistant to ampicillin but susceptible to meropenem, doripenem, ertapenem, and imipenem. Conclusion: These findings suggest a high prevalence of drug-resistant bacteria in LPD water. This is a cause for concern, as it could spread antibiotic-resistant infections in the community. Keywords: antibiotic resistance, carbapenemase, Raoultella ornithinolytica, waterborne pathogens.

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Section: Discussionmentioning

confidence: 99%

Emergence of NDM-1-producing Raoultella ornithinolytica from reservoir water in Northeast Thailand

Karnmongkol,

Wiriyaampaiwong,

Teerakul

et al. 2023

Vet World

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“…Interestingly, it was found that the presence of the blaOXA-4 gene is significantly associated with resistance to cefepime. In a study in Hamadan, Iran, Sezadehghani et al identified frequency carbapenem encoding genes (OXA), including blaOXA-145 (27.5%), blaOXA-224 (22.0%), blaOXA-539 (20.1%), and blaOXA-675 (11.9%) in clinical isolates of P. aeruginosa, which exhibited MDR (30). In a study by Radmehr et al in North Khorasan, Iran, the frequency of blaOXA-23 gene was reported 61.42% (31).…”

Section: Discussionmentioning

confidence: 99%

Genotypic Investigation of Antibiotic Resistant blaOXA-4 Gene in Clinical Isolates of Pseudomonas aeruginosa

Gholampour Matin,

Shapouri,

Nahaei

et al. 2023

Avicenna J Clin Microbiol Infect

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Background: Pseudomonas aeruginosa, an opportunistic Gram-negative bacterium, is responsible for 10-15% of hospital infections worldwide. The acquisition of resistance genes is one of the important mechanisms that causes the spread of resistance in this bacterium. This study aimed to conduct a phenotypic and genotypic investigation of the blaOXA-4 resistance gene in P. aeruginosa isolated from clinical samples. Methods: In this study, 110 P. aeruginosa strains were isolated from various clinical samples. The disk diffusion method was applied to reveal the resistance pattern in the isolates. Moreover, the combined disk method was used for the phenotypic analysis of extended-spectrum beta-lactamases (ESBL). Finally, the presence of the blaOXA-4 beta-lactamase gene was analyzed genotypically by polymerase chain reactions (PCR) method. Results: The highest sensitivity and resistance of the isolates were related to amikacin (65.45%) and ceftazidime (86.36%), respectively. The phenotypic analysis indicated that 72 isolates (65.45%) of P. aeruginosa are ESBL-producing. Furthermore, the presence of blaOXA-4 was approved genotypically in 33 P. aeruginosa isolates (45.83%). Conclusion: This study revealed a high prevalence of antibiotic-resistant isolates of P. aeruginosa in the East Azerbaijan population that may be associated with the presence of the blaOXA-4 gene. However, further studies are necessary to identify other resistant genes in ESBL-producing isolates and other geographical areas with larger sample size.

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“…30 Based on research by Sezahdeghani, et al, P. aeruginosa isolates were 40.3%, 43.1%, and 44.9% resistant to meropenem, ertapenem, and imipenem, respectively. 31 Carbapenemases are βlactamases and include serine-β-lactamases (KPC, OXA, and GES genes) and metallo-β-lactamases (MBL). Approximately 15.5% of the isolates produced strong biofilms, 37.6% of the isolates produced moderate biofilms, and 10.2% of the isolates produced weak biofilms.…”

Section: Biofilm Formationmentioning

confidence: 99%

Pseudomonas aeruginosa biofilm formation and its resistance to beta-lactam antibiotics

Gozali,

Tjampakasari

2023

DJM

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Introduction: An opportunistic pathogen, Pseudomonas aeruginosa is a Gram-negative bacteria that causes acute and chronic human infections. By adhering to appropriate surfaces and creating a biofilm matrix, Pseudomonas aeruginosa has a high-resistance structure. Due to biofilm resistance mechanisms, this bacterial biofilm may increase natural antibiotic resistance. Antibiotics have difficulty penetrating the exopolysaccharide matrix that makes up the biofilm structure (Psl, Pe, alginate, and eDNA). Alginate is involved in generating mucus, and Psl and Pel components are implicated in biofilm development and antibiotic resistance. Due to the connection between biofilm production and antibiotic resistance, Pseudomonas aeruginosa involves mechanisms of beta-lactam antibiotic resistance. Objective: On this basis, learning more about the connection between Pseudomonas aeruginosa bacteria's ability to form biofilms and their resistance to beta-lactam antibiotics is essential. Methods: We use literature methods from various literature on the biofilm formation of P. aeruginosa and its resistance to beta-lactam antibiotics, including research papers and studies with no restrictions types of studies included in this article. Discussion: Pseudomonas aeruginosa has intrinsic and adaptive antibiotic resistance mechanisms. Decreased membrane permeability, the production of enzymes resistant to antibiotics, chromosomal changes, and horizontal gene transfer from other bacteria contribute to antimicrobial resistance. Beta-lactam antibiotic resistance mechanisms are known to be developed by Pseudomonas aeruginosa. The resistance characteristics of Pseudomonas aeruginosa may change phenotypically due to biofilm development. This article will be helpful in future research on Pseudomonas aeruginosa therapy and medication.

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Detection of blaOXA-145, blaOXA-224, blaOXA-539, and blaOXA-675 Genes and Carbapenem-Hydrolyzing Class D β-Lactamases (CHDLs) in Clinical Isolates of Pseudomonas aeruginosa Collected from West of Iran, Hamadan

Cited by 3 publications

References 30 publications

Emergence of NDM-1-producing Raoultella ornithinolytica from reservoir water in Northeast Thailand

Emergence of NDM-1-producing Raoultella ornithinolytica from reservoir water in Northeast Thailand

Genotypic Investigation of Antibiotic Resistant blaOXA-4 Gene in Clinical Isolates of Pseudomonas aeruginosa

Pseudomonas aeruginosa biofilm formation and its resistance to beta-lactam antibiotics

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