Detection of Blastocystis sp. in pigs in Aragon (Spain)

Quílez, Joaquín; Clavel, Antonio; Sánchez-Acedo, Caridad; Causapé, A. C.

doi:10.1016/0304-4017(94)00682-3

Cited by 31 publications

(18 citation statements)

References 10 publications

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“…In general, however, the infection rate of animals is reduced with increasing age on pig farms. Thus, these data support the theory that adult pigs are parasitized to a lesser extent (Sanford, 1987; Quílez et al, 1996; Guselle et al, 2003; Maddox-Hyttel et al, 2006; Vítovec et al, 2006; Hamnes et al, 2007). …”

Section: Discussionsupporting

confidence: 83%

Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe

Němejc

Sak

Květoňová

et al. 2013

Veterinary Parasitology

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From 2011 to 2012, to identify Cryptosporidium spp. occurrence in Eurasian wild boars (Sus scrofa) 29 randomly selected localities (both forest areas and enclosures) across the Central European countries of Austria, the Czech Republic, Poland, and the Slovak Republic were investigated. Cryptosporidium oocysts were microscopicaly detected in 11 out of 460 faecal samples examined using aniline-carbol-methyl violet staining. Sixty-one Cryptosporidium infections, including the 11 infections that were detected by microscopy, were detected using genus- or species-specific nested PCR amplification of SSU rDNA. This represents a 5.5 fold greater sensitivity for PCR relative to microscopy. Combining genus-and species-specific PCR tools significantly changes the perspective on the occurrence of Cryptosporidium spp. in wild boars. While RFLP and direct sequencing of genus specific PCR-amplified products revealed 56 C. suis (20) and C. scrofarum (36) monoinfections and only 5 mixed infections of these species, species-specific molecular tools showed 44 monoinfections and 17 mixed infections with these species. PCR analysis of the gp60 gene did not reveal any other Cryptosporidium infections. Similar to domestic pigs, C. scrofarum was detected as a dominant species infecting adult Eurasian wild boars (Sus scrofa). Cryptosporidium infected wild boars did not show signs of clinical disease. This report is perhaps the most comprehensive survey of cryptosporidial infection in wild boars.

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Section: Discussionsupporting

confidence: 83%

Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe

Němejc

Sak

Květoňová

et al. 2013

Veterinary Parasitology

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“…One phylotype was affiliated to Blastocystis sp. This parasite has been detected previously by light microscopy in pig faeces and pigs with diarrhoea but no correlation was found between intensity of Blastocystis infection and the occurrence of diarrhoea [44,45].…”

Section: Discussionmentioning

confidence: 60%

Characterisation of the microbial diversity in a pig manure storage pit using small subunit rDNA sequence analysis

Snell‐Castro

Godon

Delgenès

et al. 2005

FEMS Microbiology Ecology

145

103

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The microbial community structure of pig manure slurry (PMS) was determined with comparative analysis of 202 bacterial, 44 archaeal and 33 eukaryotic small subunit (SSU) rDNA partial sequences. Based on a criterion of 97% of sequence similarity, the phylogenetic analyses revealed a total of 108, eight and five phylotypes for the Bacteria, Archaea and Eukarya lineages, respectively. Only 36% of the bacterial phylotypes were closely related (>or=97% similarity) to any previously known sequence in databases. The bacterial groups most often represented in terms of phylotype and clone abundance were the Eubacterium (22% of total sequences), the Clostridium (15% of sequences), the Bacillus-Lactobacillus-Streptococcus subdivision (20% of sequences), theMycoplasma and relatives (10% of sequences) and the Flexibacter-Cytophaga-Bacteroides (20% of sequences). The global microbial community structure and phylotype diversity show a close relationship to the pig gastrointestinal tract ecosystem whereas phylotypes from the Acholeplasma-Anaeroplasma and the Clostridium purinolyticum groups appear to be better represented in manure. Archaeal diversity was dominated by three phylotypes clustering with a group of uncultured microorganisms of unknown activity and only distantly related to the Thermoplasmales and relatives. Other Archaea were methanogenic H2/CO2 utilisers. No known acetoclastic Archaea methanogen was found. Eukaryotic diversity was represented by a pluricellular nematode, two Alveolata, a Blastocystis and an Entamoebidae. Manure slurry physico-chemical characteristics were analysed. Possible inhibitory effects of acetate, sulphide and ammonia concentrations on the microbial anaerobic ecosystem are discussed.

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“…hominis-like organisms has been found in several animal kinds (Abe et al 2002(Abe et al , 2003Boreham and Stenzel 1993;Duda et al 1998;Quílez et al 1993) and the description of new Blastocystis species has been accomplished attending to the host species and few biological characteristics (Belova and Kostenko 1990;Chen et al 1997;Krylov and Belova 1997), criteria considered as insufficient for speciation (Yoshikawa et al 1998). Nevertheless, some of them have singular phenetic characteristics that make them different from those found in humans and other homeothermic animals, such happens with isolates from snakes (Blastocystis lapemi and Blastocystis pythoni), iguana (Blastocystis cycluri) and tortoise (Blastocystis geocheloni) (Teow et al 1991;Singh et al 1996).…”

Section: Introductionmentioning

confidence: 99%

High prevalence of subtype 4 among isolates of Blastocystis hominis from symptomatic patients of a health district of Valencia (Spain)

et al. 2009

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In order to know the genetic diversity of Blastocystis hominis from a health district of Valencia (Spain) 51 clinical isolates from symptomatic patients, 31 axenic and 20 monoxenic, were ribotyped by analysing the restriction fragment length polymorphism (RFLP) of amplicons obtained by polymerase chain reaction (PCR) of small-subunit of ribosomal DNA genes (SSU-rDNA). For this purpose, DNA was subjected to two independent PCR (RD3-RD5, F1-R1) and to three independent treatments with restrictases (AluI, HinfI and RsaI). The digested DNA was separated electrophoretically, the isolates were clustered into ribotypes (ribodemes, RD3-RD5; subgroups, F1-R1) according to their profiles and the results were translated into genetic subtypes (ST) proposed by a consensus terminology. The results show that the isolates studied are an heterogeneous population and that both PCR-RFLP SSU-rDNA protocols have a similar discriminative power, since it allowed the ribotyping of all isolates and their clustering into four demes: ribodemes 1, 3 and 3-r and 6, which include isolates belonging to subgroup III, IV, V and V-r, respectively; which were assigned to ST1 (2%), ST2 (3.9%) and ST4 (94.1%). The most common of which is a zoonotic subtype (Blastocystis ratti) which includes, according to recent studies, non-pathogenic and pathogenic variants.

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Detection of Blastocystis sp. in pigs in Aragon (Spain)

Cited by 31 publications

References 10 publications

Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe

Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe

Characterisation of the microbial diversity in a pig manure storage pit using small subunit rDNA sequence analysis

High prevalence of subtype 4 among isolates of Blastocystis hominis from symptomatic patients of a health district of Valencia (Spain)

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