Detection of blaVIM-7 in an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST1284 in Brazil

Paula, Suelen Balero de; Cayô, Rodrigo; Streling, Ana Paula; Nodari, Carolina Silva; Matos, Adriana Pereira; Perugini, Marcia Regina Eches; Gales, Ana Cristina; Carrara-Marroni, Floristher Elaine; Yamada‐Ogatta, Sueli Fumie

doi:10.1016/j.diagmicrobio.2017.06.008

“…It shares only a 77% identity with VIM-1 and 74% with VIM-2, and lies on a 24-kb plasmid, which can be readily transferred into Enterobacteriaceae and other pseudomonads, and was thought to originate from a different ancestral source. After original detection in North America, VIM-7 was recently described in another report from Brazil [90]. Our group has lately detected several VIM enzymes including VIM-2, VIM-30, VIM-31, and VIM-42 from nosocomial P. aeruginosa in Dubai, United Arab Emirates [67], with VIM-2 co-existing with the Ambler class A carbapenemase GES-5.…”

Section: Ambler Class B Carbapenemases (Metallo-β-lactamases)mentioning

confidence: 69%

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

Halat

¹

,

Moubareck

²

2020

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Carbapenemases are β-lactamases belonging to different Ambler classes (A, B, D) and can be encoded by both chromosomal and plasmid-mediated genes. These enzymes represent the most potent β-lactamases, which hydrolyze a broad variety of β-lactams, including carbapenems, cephalosporins, penicillin, and aztreonam. The major issues associated with carbapenemase production are clinical due to compromising the activity of the last resort antibiotics used for treating serious infections, and epidemiological due to their dissemination into various bacteria across almost all geographic regions. Carbapenemase-producing Enterobacteriaceae have received more attention upon their first report in the early 1990s. Currently, there is increased awareness of the impact of nonfermenting bacteria, such as Acinetobacter baumannii and Pseudomonas aeruginosa, as well as other Gram-negative bacteria that are carbapenemase-producers. Outside the scope of clinical importance, carbapenemases are also detected in bacteria from environmental and zoonotic niches, which raises greater concerns over their prevalence, and the need for public health measures to control consequences of their propagation. The aims of the current review are to define and categorize the different families of carbapenemases, and to overview the main lines of their spread across different bacterial groups.

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“…(15) Often, these strains cause regional or nationwide outbreaks, express MDR phenotypes, and are associated with high mortality. (31, 37, 38) VIM-producing ST1284 P. aeruginosa have been described from Brazil, (39), and ST389 from cystic brosis patients in Italy. (40) Both sequence types have otherwise limited geographic distribution.…”

Section: Discussionmentioning

confidence: 99%

β-lactamase-mediated Resistance in MDR-Pseudomonas Aeruginosa from Qatar

Ahmed

¹

,

Khan

²

,

Sultan

³

et al. 2020

Preprint

0

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Background: Anti-pseudomonal b-lactam antibiotics are of paramount importance in the management of Pseudomonas aeruginosa infections. The distribution of b-lactam resistance genes in P. aeruginosa is often closely related to the distribution of certain high-risk international clones. In the present study whole-genome sequencing (WGS) was used to identify the predominant sequence types (STs) and b-lactamase genes in clinical isolates of multidrug-resistant (MDR)-P. aeruginosa from QatarMethods: Microbiological identification and susceptibility tests were performed by automated BD PhoenixTM system and manual Liofilchem MIC Test Strips. Seventy-five MDR-P. aeruginosa isolates were subsequently processed for WGS. Results: Among 75 MDR-P. aeruginosa isolates; the largest proportions of susceptibility were to ceftazidime-avibactam (n=36, 48%), followed by ceftolozane-tazobactam (30, 40%), ceftazidime (n=21, 28%) and aztreonam (n=16, 21.33%). Almost all isolates possessed Class C and Class D b-lactamases (n=72, 96% each), while metallo-β-lactamases were detected in 20 (26.67%) isolates. Eight (40%) metallo-β-lactamases producers were susceptible to aztreonam and did not produce any concomitant extended-spectrum b-lactamases. The most frequent sequence types identified were ST235 (n=16, 21.33%), ST357 (n=8, 10.67%), ST389 and ST1284 (6, 8% each). Nearly all ST235 (15/16; 93.75%) were resistant to all tested b-lactams.Conclusion: MDR-P. aeruginosa isolates from Qatar are highly resistant to antipseudomonal b-lactams. Global high-risk STs are predominant in Qatar and their associated multi-resistant phenotypes are a cause for considerable concern. The molecular epidemiology trends of P. aeruginosa should be surveillance at national levels to track their trends and institute the appropriate control strategies.

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“…(15) Often, these strains cause regional or nationwide outbreaks, express MDR phenotypes, and are associated with high mortality. (31,37,38) VIM-producing ST1284 P. aeruginosa have been described from Brazil, (39), and ST389 from cystic brosis patients in Italy. (40) Both sequence types have otherwise limited geographic distribution.…”

Section: Discussionmentioning

confidence: 99%

β-Lactamase-Mediated Resistance in MDR-Pseudomonas Aeruginosa from Qatar

Ahmed

¹

,

Khan

²

,

Sultan

³

et al. 2020

Preprint

1

0

View full text Add to dashboard Cite

Background: Anti-pseudomonal b-lactam antibiotics are of paramount importance in the management of Pseudomonas aeruginosa infections. The distribution of b-lactam resistance genes in P. aeruginosa is often closely related to the distribution of certain high-risk international clones. In the present study whole-genome sequencing (WGS) was used to identify the predominant sequence types (STs) and b-lactamase genes in clinical isolates of multidrug-resistant (MDR)-P. aeruginosa from QatarMethods: Microbiological identification and susceptibility tests were performed by automated BD PhoenixTM system and manual Liofilchem MIC Test Strips. Seventy-five MDR-P. aeruginosa isolates were subsequently processed for WGS. Results: Among 75 MDR-P. aeruginosa isolates; the largest proportions of susceptibility were to ceftazidime-avibactam (n=36, 48%), followed by ceftolozane-tazobactam (30, 40%), ceftazidime (n=21, 28%) and aztreonam (n=16, 21.33%). Almost all isolates possessed Class C and Class D b-lactamases (n=72, 96% each), while metallo-β-lactamases were detected in 20 (26.67%) isolates. Eight (40%) metallo-β-lactamases producers were susceptible to aztreonam and did not produce any concomitant extended-spectrum b-lactamases. The most frequent sequence types identified were ST235 (n=16, 21.33%), ST357 (n=8, 10.67%), ST389 and ST1284 (6, 8% each). Nearly all ST235 (15/16; 93.75%) were resistant to all tested b-lactams.Conclusion: MDR-P. aeruginosa isolates from Qatar are highly resistant to antipseudomonal b-lactams. Global high-risk STs are predominant in Qatar and their associated multi-resistant phenotypes are a cause for considerable concern. The molecular epidemiology trends of P. aeruginosa should be surveillance at national levels to track their trends and institute the appropriate control strategies.

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Detection of blaVIM-7 in an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST1284 in Brazil

Abstract: We described for the first time an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST1284 carrying a plasmid-mediated bla in Brazil. The bla was harbored by an integron that also carried aacA4 and bla. Multiple virulence factors were also detected.

Cited by 12 publications

References 22 publications

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

β-lactamase-mediated Resistance in MDR-Pseudomonas Aeruginosa from Qatar

β-Lactamase-Mediated Resistance in MDR-Pseudomonas Aeruginosa from Qatar

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Detection of blaVIM-7 in an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST1284 in Brazil

Abstract: We described for the first time an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST1284 carrying a plasmid-mediated bla in Brazil. The bla was harbored by an integron that also carried aacA4 and bla. Multiple virulence factors were also detected.

Cited by 12 publications

References 22 publications

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

β-lactamase-mediated Resistance in MDR-Pseudomonas Aeruginosa from Qatar

β-Lactamase-Mediated Resistance in MDR-Pseudomonas Aeruginosa&nbsp;from Qatar

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β-Lactamase-Mediated Resistance in MDR-Pseudomonas Aeruginosa from Qatar