Detection of Bordetella pertussis by polymerase chain reaction and culture in the nasopharynx of erythromycin-treated infants with pertussis

Edelman, Kati; Nikkari, Simo; Ruuskanen, Olli; He, Qiushui; Viljanen, Matti K.; Mertsola, Jussi

doi:10.1097/00006454-199601000-00012

Cited by 45 publications

(14 citation statements)

References 15 publications

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“…The persistence of pertussis DNA after erythromycin therapy in nine infants has already been described by Edelman et al (5). Pertussis PCR remained positive for 7 days in four patients and for 10 days in two patients.…”

supporting

confidence: 62%

Treatment Failure of Nosocomial Pertussis Infection in a Very-Low-Birth-Weight Neonate

et al. 2006

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supporting

confidence: 62%

Treatment Failure of Nosocomial Pertussis Infection in a Very-Low-Birth-Weight Neonate

et al. 2006

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“…The persistent presence of B. pertussis DNA has been detected by PCR after treatment of patients with antibiotics for up to 21 days (80,156).…”

Section: Pcr Assaysmentioning

confidence: 99%

Laboratory Diagnosis of Pertussis

2015

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SUMMARYThe introduction of vaccination in the 1950s significantly reduced the morbidity and mortality of pertussis. However, since the 1990s, a resurgence of pertussis has been observed in vaccinated populations, and a number of causes have been proposed for this phenomenon, including improved diagnostics, increased awareness, waning immunity, and pathogen adaptation. The resurgence of pertussis highlights the importance of standardized, sensitive, and specific laboratory diagnoses, the lack of which is responsible for the large differences in pertussis notifications between countries. Accurate laboratory diagnosis is also important for distinguishing between the several etiologic agents of pertussis-like diseases, which involve both viruses and bacteria. If pertussis is diagnosed in a timely manner, antibiotic treatment of the patient can mitigate the symptoms and prevent transmission. During an outbreak, timely diagnosis of pertussis allows prophylactic treatment of infants too young to be (fully) vaccinated, for whom pertussis is a severe, sometimes fatal disease. Finally, reliable diagnosis of pertussis is required to reveal trends in the (age-specific) disease incidence, which may point to changes in vaccine efficacy, waning immunity, and the emergence of vaccine-adapted strains. Here we review current approaches to the diagnosis of pertussis and discuss their limitations and strengths. In particular, we emphasize that the optimal diagnostic procedure depends on the stage of the disease, the age of the patient, and the vaccination status of the patient.

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“…The timing of specimen acquisition may also be relevant, since the average delay between onset of illness and specimen collection was 9 days for our PCR-positive cases versus 16 days for all cases studied with broad-range PCR. Although bacterial DNA persists longer than cultivatable organisms after therapy is initiated ( 27 ), the former still has a limited half-life. Finally, it must be kept in mind that some of these cases may not have a microbial cause.…”

Section: Discussionmentioning

confidence: 99%

Broad-Range Bacterial Detection and the Analysis of Unexplained Death and Critical Illness

Nikkari

Lopez²,

Lepp³

et al. 2002

Emerg. Infect. Dis.

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Broad-range rDNA polymerase chain reaction (PCR) provides an alternative, cultivation-independent approach for identifying pathogens. In 1995, the Centers for Disease Control and Prevention initiated population-based surveillance for unexplained life-threatening infections (Unexplained Death and Critical Illness Project [UNEX]). To address the causes of UNEX cases, we examined 59 specimens from 46 cases by using broad-range bacterial 16S rDNA PCR and phylogenetic analysis of amplified sequences. Specimens from eight cases yielded sequences from Neisseria meningitidis (cerebrospinal fluid from two patients with meningitis), Streptococcus pneumoniae (cerebrospinal fluid from one patient with meningitis and pleural fluid from two patients with pneumonia), or Stenotrophomonas maltophilia (bone marrow aspirate from one patient with pneumonia). Streptococcus pneumoniae rDNA sequence microheterogeneity was found in one pleural fluid specimen, suggesting the presence of multiple strains. In conclusion, known bacterial pathogens cause some critical illnesses and deaths that fail to be explained with traditional diagnostic methods.

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Detection of Bordetella pertussis by polymerase chain reaction and culture in the nasopharynx of erythromycin-treated infants with pertussis

Abstract: The results of this study show that PCR assay can achieve the specific diagnosis of pertussis infection in a large proportion of infants even when antimicrobial treatment has killed the organisms and culture is no longer positive.

Cited by 45 publications

References 15 publications

Treatment Failure of Nosocomial Pertussis Infection in a Very-Low-Birth-Weight Neonate

Treatment Failure of Nosocomial Pertussis Infection in a Very-Low-Birth-Weight Neonate

Laboratory Diagnosis of Pertussis

Broad-Range Bacterial Detection and the Analysis of Unexplained Death and Critical Illness

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