Detection of Borna disease virus RNA in formalin-fixed, paraffin-embedded brain tissues by nested PCR

Sorg, Ingrid; Metzler, A. E.

doi:10.1128/jcm.33.4.821-823.1995

Cited by 37 publications

(13 citation statements)

References 30 publications

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“…Primer design. Among different primer pairs tested, the one that proved to be best for the detection of classical BDV and a highly variant strain, described by Sorg & Metzler (1995), was used for screening PCR. Furthermore, by using the Primer Designer program (version 3.0; Scientific & Educational Software), six primer pairs were designed in order to amplify overlapping PCR products that comprised the complete N, X and P protein-encoding regions of the genome, as well as the 59-untranslated region of the X/P transcript, and were synthesized by Invitrogen.…”

Section: Methodsmentioning

confidence: 99%

Genetic clustering of Borna disease virus natural animal isolates, laboratory and vaccine strains strongly reflects their regional geographical origin

Kolodziejek

Dürrwald²,

Herzog

et al. 2005

Journal of General Virology

109

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The aim of this study was to gain more detailed insights into the genetic evolution and variability of Borna disease virus (BDV). Phylogenetic analyses were performed on field viruses originating from naturally infected animals, the BDV vaccine strain ‘Dessau’, four widely used laboratory strains and the novel BDV subtype No/98. Four regions of the BDV genome were analysed: the complete p40, p10 and p24 genes and the 5′-untranslated region of the X/P transcript. BDV isolates from the same geographical area exhibited a clearly higher degree of identity to each other than to BDV isolates from other regions, independent of host species and year of isolation. Five different clusters could be established within endemic areas, corresponding to the geographical regions from which the viruses originated: (i) a Swiss, Austrian and Liechtenstein Rhine valley group, related closely to the geographically bordering Baden-Wurttemberg and Bavaria II group (ii) in the western part of Germany; (iii) a third group, called Bavaria I group, limited in occurrence to Bavaria; (iv) a southern Saxony-Anhalt and bordering northern Saxony group, bound to the territories of these federal states in the eastern part of Germany; and (v) a mixed group, consisting of samples from different areas of Germany; however, these were mainly from the federal states of Thuringia and Lower Saxony. The laboratory strains and the vaccine strain clustered within these groups according to their geographical origins. All field and laboratory strains, as well as the vaccine strain, clearly segregated from the recently described and highly divergent BDV strain No/98, which originated from an area in Austria where Borna disease is not endemic.

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Section: Methodsmentioning

confidence: 99%

Genetic clustering of Borna disease virus natural animal isolates, laboratory and vaccine strains strongly reflects their regional geographical origin

Kolodziejek

Dürrwald²,

Herzog

et al. 2005

Journal of General Virology

109

View full text Add to dashboard Cite

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“…Viral DNA was extracted with two different commercial kits depending on the type of sample: a QIAamp DNA FFPE Tissue kit (Qiagen, CA, USA) was used for formalin-fixed and paraffin-embedded livers, and a NucleoSpin Blood kit (Macherey-Nagel, GmbH & Co. KG, Germany) was used for frozen tissues. When DNA had to be extracted from paraffin-embedded tissues, samples were deparaffinized following the protocol described by Sorg & Metzler (1995). DNA extraction was performed according to the protocol supplied by the manufacturers.…”

Section: Fadv Detection By Pcr and Sequencingmentioning

confidence: 99%

Epidemiological and pathological investigation of fowl aviadenovirus serotypes 8b and 11 isolated from chickens with inclusion body hepatitis in Spain (2011–2013)

et al. 2016

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Inclusion body hepatitis caused by different fowl aviadenovirus (FAdV) serotypes has been described in several countries in recent years. In Spain, from the spring of 2011 to 2013, an increased number of outbreaks in broiler and broiler breeder flocks from different regions occurred. The objectives of the present work were to carry out the molecular characterization of FAdV strains from Spanish inclusion body hepatitis cases and to study the pathogenicity and viral dynamics of these strains in specific pathogen-free (SPF) chickens. A total of 52 inclusion body hepatitis clinical cases, including 45 from broiler farms and seven from broiler breeder farms, were analysed by conventional polymerase chain reaction and sequencing targeting the FAdV hexon gene. From these, 37 strains were classified as FAdV type 8b, while the remaining 15 were classified as FAdV types 11 (n = 10), 2 (n = 4) and 8a (n = 1). In addition, two different FAdVs belonging to the genotypes 8b and 11 were used for experimental infection. Specific pathogen-free five-day-old birds were inoculated intramuscularly with a high (10 tissue culture infective dose (TCID)/ml) or low (10 TCID/ml) dose of the above-mentioned FAdVs. No mortality was observed in any of the experimental groups, and only one bird showed evident clinical signs. However, macroscopic and microscopic hepatic lesions, as well as viral DNA, were detected in birds from all infection groups. Inclusion bodies and viral DNA were also detected in the pancreas and in the small and the large intestine in some birds. Long-lasting shedding and transmission to contact birds were confirmed in all infected groups.

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“…However, the seroprevalence of BDV infections in different studies have shown controversial results. Recently, molecular assays for the detection of BDV are commonly used because they provide highly specific and sensitive results in a timely manner [11,12]. The use of reverse transcriptase PCR (RT‐PCR) to detect BDV RNA in peripheral blood mononuclear cells (PBMCs) of infected subjects is a powerful tool to evaluate the role of carrier hosts as potential sources of human BDV infection [13,14].…”

Section: Introductionmentioning

confidence: 99%

Detection and analysis of Borna disease virus in Chinese patients with neurological disorders

Wang

Zhu

et al. 2009

Euro J of Neurology

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Detection of Borna disease virus RNA in formalin-fixed, paraffin-embedded brain tissues by nested PCR

Cited by 37 publications

References 30 publications

Genetic clustering of Borna disease virus natural animal isolates, laboratory and vaccine strains strongly reflects their regional geographical origin

Genetic clustering of Borna disease virus natural animal isolates, laboratory and vaccine strains strongly reflects their regional geographical origin

Epidemiological and pathological investigation of fowl aviadenovirus serotypes 8b and 11 isolated from chickens with inclusion body hepatitis in Spain (2011–2013)

Detection and analysis of Borna disease virus in Chinese patients with neurological disorders

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