Detection of Botryosphaeriaceae species within grapevine woody tissues by nested PCR, with particular emphasis on the Neofusicoccum parvum/N. ribis complex

Spagnolo, Alessandro; Marchi, Guido; Peduto, F.; Phillips, Alan J. L.; Surico, Giuseppe

doi:10.1007/s10658-010-9715-9

Cited by 35 publications

(27 citation statements)

References 23 publications

(34 reference statements)

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“…This is due to the fact that Neofusicoccum parvum is the anamorph of Botryosphaeria parva [20]. N. parvum and N. ribis were originally considered to be part of the Botryosphaeria dothidea complex [21].…”

Section: Resultsmentioning

confidence: 99%

Growth inhibition of an Araucaria angustifolia (Coniferopsida) fungal seed pathogen, Neofusicoccum parvum, by soil streptomycetes

et al. 2013

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BackgroundAraucariaceae are important forest trees of the southern hemisphere. Life expectancy of their seedlings can largely be reduced by fungal infections. In this study we have isolated and characterized such a fungus and investigated the potential of Streptomyces Actinobacteria from the respective rhizosphere to act as antagonists.ResultsThe pathogenic fungus from Araucaria angustifolia seeds was identified by morphological markers (pore-associated Woronin-bodies) as belonging to the Pezizomycotina. Molecular data identified the fungus as Neofusicoccum parvum (Botryosphaeriaceae). Co-cultures on agar of this fungus with certain streptomycete isolates from the rhizosphere, and from the surface of Araucaria roots significantly reduced the growth of the fungus. HPLC analysis of the agar yielded streptomycete-specific exudate compounds which were partly identified. There were differences in compounds between single (bacteria, fungus) and dual cultures (bacteria + fungus).ConclusionStreptomycetes from the rhizosphere of Araucariaceae produce exudates which can suppress the development of pathogenic fungi in their seeds.

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Section: Resultsmentioning

confidence: 99%

Growth inhibition of an Araucaria angustifolia (Coniferopsida) fungal seed pathogen, Neofusicoccum parvum, by soil streptomycetes

et al. 2013

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“…Although the exact cause of these chloro-necrotic foliar symptoms is still unclear [5], they could be the result, wholly or in part, of the synergistic action of the toxic metabolites produced by species of Botryosphaeriaceae and Pa. chlamydospora [132]. …”

Section: Botryosphaeriaceae Species Involved In Grapevine Trunk DImentioning

confidence: 99%

Phytotoxins Produced by Fungi Associated with Grapevine Trunk Diseases

Andolfi

Mugnai

Luque

et al. 2011

Toxins

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Up to 60 species of fungi in the Botryosphaeriaceae family, genera Cadophora, Cryptovalsa, Cylindrocarpon, Diatrype, Diatrypella, Eutypa, Eutypella, Fomitiporella, Fomitiporia, Inocutis, Phaeoacremonium and Phaeomoniella have been isolated from decline-affected grapevines all around the World. The main grapevine trunk diseases of mature vines are Eutypa dieback, the esca complex and cankers caused by the Botryospheriaceae, while in young vines the main diseases are Petri and black foot diseases. To understand the mechanism of these decline-associated diseases and the symptoms associated with them, the toxins produced by the pathogens involved in these diseases were isolated and characterised chemically and biologically. So far the toxins of only a small number of these decline fungi have been studied. This paper presents an overview of the toxins produced by the most serious of these vine wood pathogens: Eutypa lata, Phaeomoniella chlamydospora, Phaeoacremonium aleophilum and some taxa in the Botryosphaeriaceae family, and examines how these toxins produce decline symptoms. The chemical structure of these metabolites and in some cases their vivotoxin nature are also discussed.

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“…In New Zealand, Botryosphaeriaceae species were frequently isolated from failed young plants (I. Harvey, Plantwise, New Zealand, personal communication). Spagnolo et al (2011) also detected these pathogens within standing vine wood samples and dormant grafted plants from different Italian and French nurseries using two Botryopshaeriaceae-specific nested PCR assays. These findings indicate that infected planting materials may have been used in the propagation process.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and distribution of Botryosphaeriaceae species in New Zealand grapevine nurseries

et al. 2012

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To determine presence of infection by botryosphaeriaceous fungi in plant materials from different grapevine nurseries, different types of grafted plants and cuttings were requested from nine grapevine nurseries around New Zealand. Of the 311 propagation materials and plants collected, 23 % were infected by botryosphaeriaceous fungi. The highest incidence was in failed grafted plants (33 %), followed by Grade 1 plants (28 %), rootstock cuttings (19 %), scion cuttings (17 %) and Grade 2 plants (7 %). For grafted plants, most isolates (80 %) were from near the graft unions while infections on rootstocks and scion cuttings were mostly from the middle (39 %) and basal parts (46 %). Identification of isolates by morphological and molecular methods showed that seven species were present in nurseries, being Neofusicoccum luteum (57 %), N. parvum (18 %), N. australe (8 %), Diplodia mutila (8 %), Botryosphaeria dothidea (5 %) and D. seriata (3 %), with one novel isolate of N. macroclavatum. These results show that Botryosphaeriaceae species were present in propagation materials and in the grafted plants supplied by nurseries, indicating that some of the current vineyard infections may have originated in the propagation nurseries.

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Detection of Botryosphaeriaceae species within grapevine woody tissues by nested PCR, with particular emphasis on the Neofusicoccum parvum/N. ribis complex

Abstract: Several species of Botryosphaeriaceae and

Cited by 35 publications

References 23 publications

Growth inhibition of an Araucaria angustifolia (Coniferopsida) fungal seed pathogen, Neofusicoccum parvum, by soil streptomycetes

Growth inhibition of an Araucaria angustifolia (Coniferopsida) fungal seed pathogen, Neofusicoccum parvum, by soil streptomycetes

Phytotoxins Produced by Fungi Associated with Grapevine Trunk Diseases

Prevalence and distribution of Botryosphaeriaceae species in New Zealand grapevine nurseries

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