Detection of Bovine Herpesvirus 1 Glycoprotein E Antibodies in Individual Milk Samples by Enzyme-Linked Immunosorbent Assays

Abstract

The purpose of this study was to determine whether individual milk samples can replace serum samples for the detection of bovine herpesvirus 1 (BHV1) glycoprotein E (gE)-specific antibodies. Serum and milk samples were collected at the same time from cattle in BHV1-free herds, cattle in unvaccinated herds, and cattle in herds that were vaccinated twice with a BHV1 marker vaccine. The samples were tested in two gE enzyme-linked immunosorbent assay (ELISA) systems. In comparison to serum, the results showed that the gE-blocking ELISA was highly sensitive for testing milk samples (0.96). In contrast, the gE ELISA was less sensitive (0.79). The specificities of the gE-blocking ELISA and the gE ELISA for testing milk samples were very high (1.00 and 0.99, respectively). The presented results indicate that individual milk samples, which can be collected relatively easily and inexpensively, can be used instead of individual serum samples in the gE-blocking ELISA for the screening of cattle for BHV1 gE antibodies.

Presence of bovine herpesvirus 1 gB‐seropositive but gE‐seronegative Dutch cattle with no apparent virus exposure

“…All the sera from the field trial were tested with a gE‐blocking elisa (Wellenberg and others 1998). Sera from the placebo‐treated cattle were also tested with a gB‐blocking elisa (Kramps and others 1994).…”

ELISA detection of antibodies to glycoprotein E of bovine herpesvirus 1 in bulk milk samples

“…These freeze‐dried bulk milk samples were dissolved in 10 ml of deionised water and analysed undiluted in the bhv ‐1‐gE elisa and in the bhv ‐1‐gB elisa , which was used as the standard test (Kramps and others 1994). The quality of each bhv ‐1 elisa batch was first checked and only approved batches were used in this study (Wellenberg and others 1998). All Danish bulk milk samples were negative in both elisa systems.…”

Bovine herpesvirus 1 (BHV1) seroprevalence in the breeding cattle population of the veneto region: prospects for the implementation of a control programme

“…Enhanced culling of seropositive animals and purchase restrictions are more appropriate measures in order to rapidly achieve the seronegativity of the entire stock. Moreover, the lower sensitivity of gE.ELISA in comparison with other ELISAs and the 24 h virus neutralization test (Perrin et al, 1996;De Wit et al, 1998;Hage et al, 1998;Wellenberg et al, 1998) should be mentioned; fully seronegative cattle therefore offer a greater guarantee of freedom from disease than those immunized with gE-deleted vaccine.…”