Detection of bovine herpesvirus 1 and 5 in trigeminal ganglia of beef cattle in Uruguay

Furtado, Agustín; Campos, F P F; Llambí, Silvia; Maisonnave, J.; Roehe, P. M.; Oliveira, MT; Spilki, Fernando Rosado; Franco, Ana Cláudia; Puentes, Rodrigo

doi:10.4067/s0301-732x2014000300016

Cited by 3 publications

(5 citation statements)

References 28 publications

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“…In Brazil, in that study [31], bovine herpesvirus genomes were detected in 87% of the sampled animals, of which 82.8% had BoAHV1, 93.1% had BoAHV-5 and 75.9% had both BoAHV1 and BoAHV5 genomes, suggesting a higher prevalence of BoAHV5 than BoAHV1. In Uruguay, 64.5% of the sampled cattle contained herpesvirus genomes, of which 48.4% had BoAHV1, 1.6% had BoAHV5, and 14.5% had co-infections with BoAHV1 and BoAHV5 [42]. The low prevalence of BoAHV1, BoAHV5, and BuAHV1 in tonsils suggested that, although tonsils may have served as site of infection or latency for herpesviruses, such tissues are not the primary site of latency, as was previously observed [31,42].…”

Section: Discussionsupporting

confidence: 59%

“…In Uruguay, 64.5% of the sampled cattle contained herpesvirus genomes, of which 48.4% had BoAHV1, 1.6% had BoAHV5, and 14.5% had co-infections with BoAHV1 and BoAHV5 [42]. The low prevalence of BoAHV1, BoAHV5, and BuAHV1 in tonsils suggested that, although tonsils may have served as site of infection or latency for herpesviruses, such tissues are not the primary site of latency, as was previously observed [31,42]. However, additional studies should contemplate other tissues, such as trigeminal ganglia, to provide a more adequate evaluation on the prevalence of such infections.…”

Section: Discussionmentioning

confidence: 99%

“…The fragment of the gC coding region targeted here was adequate to perform phylogenetic analyses and to allow distinction between the three viruses under study. The nPCR employed here was originally designed to differentiate BoAHV1 from BoAHV5 [56], and has been extensively used in previous studies by our group and by others [36,42,56,60]. The partial region of the gC gene sequenced here corresponds to 37.13% of the complete gC gene in BoAHV1 (567 nt), 38.60% in BoAHV5 (564 nt), and 39% in BuAHV1 (564 nt).…”

Section: Discussionmentioning

confidence: 99%

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Bovine Alphaherpesvirus 1, Bovine Alphaherpesvirus 5 and Bubaline Alphaherpesvirus 1 in Palatine Tonsils from Water Buffaloes in Northern Brazil and Possible Links with the Origin of Bovine Alphaherpesvirus Type 5

Paredes-Galarza,

Oliveira,

Timm

et al. 2024

Viruses

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Herpesviruses are significant pathogens of ruminants. In water buffaloes (Bubalus bubalis), however, herpesviruses have not been thoroughly studied. Although bubaline alphaherpesvirus 1 (BuAHV1) and bovine alphaherpesvirus 1 (BoAHV1) have already been recovered from water buffaloes, to date, no reports on the occurrence of bovine alphaherpesvirus 5 (BoAHV5) in these animals have been published. Therefore, the aim of this study was to search for BuAHV1, BoAHV1, and BoAHV5 in palatine tonsils of apparently healthy water buffaloes from the Pará state, Northern Brazil. Tissue samples of tonsils (n = 293) were screened by a nested PCR (nPCR) targeting a region of UL44 (gC coding gene), followed by sequencing, to detect and differentiate between the viral types. Viral genome segments were detected in 18 out of 293 (6.1%) of the palatine tonsil samples. Two animals carried genomes of BoAHV1 only, eleven animals carried BoAHV5 genomes only, and four animals carried BuAHV1 only. Another animal had both BoAHV1 and BoAHV5 genomes in its tonsils. No infectious virus could be recovered from any of the samples. The BuAHV1 sequences identified here were more closely related to BuAHV1 genomes identified in India. Phylogenetic analyses suggested a closer relationship between the recovered BoAHV5 and BuAHV1 genomes. Therefore, evidence is provided here to confirm that not only BoAHV1 and BuAHV1, but also BoAHV5, can infect water buffaloes. This report highlights (i) the first detection of BoAHV5 in water buffaloes and (ii) the occurrence of coinfections with BoAHV1 and BoAHV5 in that species. Such findings and the similarity of BoAHV5 to Indian herpesvirus genomes suggest that the origin of type 5 may be linked to recombinations between bovine and bubaline herpesviruses within bubalines, since the scenario for generation of recombinants in buffaloes is potentially present.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Bovine Alphaherpesvirus 1, Bovine Alphaherpesvirus 5 and Bubaline Alphaherpesvirus 1 in Palatine Tonsils from Water Buffaloes in Northern Brazil and Possible Links with the Origin of Bovine Alphaherpesvirus Type 5

Paredes-Galarza,

Oliveira,

Timm

et al. 2024

Viruses

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“…Following a typical herpesvirus pattern, they can establish longterm latent infections in their hosts, with sporadic episodes of viral shedding [3]. BoHV-1 is distributed worldwide [4] while BoHV-5 is restricted to a number of countries in South America including Brazil [5], Argentina [6], Colombia [7] and Uruguay [8]. In the northern hemisphere, the occurrence of BoHV-5 infections is rare [9,10].…”

Section: Introductionmentioning

confidence: 99%

Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle

Siedler

Roloff

Sá

et al. 2017

Protein Expression and Purification

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Bovine herpesvirus (BoHV) glycoprotein E (gE) is a non-essential envelope glycoprotein and the deletion of gE has been used to develop BoHV-1 and BoHV-5 differential vaccine strains. The DIVA (Differentiation of Infected from Vaccinated Animals) strategy, using marker vaccines based on gE-negative BoHV strains, allows the identification of vaccinated or infected animals in immunoassays designed to detect anti-gE antibodies. In this study a codon optimized synthetic sequence of gE containing highly conserved regions from BoHV-1 and BoHV-5 was expressed in Pichia pastoris. Following expression, the recombinant gE (rgE) was secreted and purified from the culture medium. The rgE was identified by Western blotting (WB) using sera from cattle naturally infected with BoHV-1 and/or BoHV-5, or sera from bovines experimentally infected with wild-type BoHV-5. Sera collected from cattle vaccinated with a BoHV-5 gI/gE/US9¯ marker vaccine failed to recognise rgE. Expression of rgE, based on a sequence containing highly conserved regions from BoHV-1 and BoHV-5, in P. pastoris enabled the production of large quantities of rgE suitable for use in immunoassays for the differentiation vaccinated or infected cattle.

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“…As with BoHV-1, the number of BoHV-5-latently infected animals, as detected by PCR in neuronal tissues, surpasses by far those which display clinical disease [ 8 , 9 ]. However, BoHV-5 infections are more drastic: when signs of disease are evident, it is usually fatal [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison between DNA Detection in Trigeminal Nerve Ganglia and Serology to Detect Cattle Infected with Bovine Herpesviruses Types 1 and 5

et al. 2016

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Bovine herpesviruses (BoHVs) types 1 (BoHV-1) and 5 (BoHV-5) are alphaherpesviruses of major importance to the bovine production chain. Such viruses are capable of establishing latent infections in neuronal tissues. Infected animals tend to develop a serological response to infection; however, such response—usually investigated by antibody assays in serum—may eventually not be detected in laboratory assays. Nevertheless, serological tests such as virus neutralization (VN) and various enzyme-linked immunosorbent assays (ELISAs) are widely employed to check individual or herd status of BoHV infections. The correlation between detection of antibodies and the presence of viral nucleic acids as indicatives of infection in infected cattle has not been deeply examined. In order to investigate such correlation, 248 bovine serum samples were tested by VN to BoHV-1 and BoHV-5, as well as in a widely employed (though not type-differential) gB ELISA (IDEXX IBR gB X2 Ab Test) in search for antibodies to BoHVs. Immediately after blood withdrawal, cattle were slaughtered and trigeminal ganglia (TG) excised for DNA extraction and viral nucleic acid detection (NAD) by nested PCR. Neutralizing antibodies to BoHV-1 and/or BoHV-5 were detected in 44.8% (111/248) of sera, whereas the gB ELISA detected antibodies in 51.2% (127/248) of the samples. However, genomes of either BoHV-1, BoHV-5, or both, were detected in TGs of 85.9% (213/248) of the animals. These findings reveal that the assays designed to detect antibodies to BoHV-1 and/or BoHV-5 employed here may fail to detect a significant number of latently infected animals (in this study, 35.7%). From such data, it is clear that antibody assays are poorly correlated with detection of viral genomes in BoHV-1 and BoHV-5-infected animals.

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Detection of bovine herpesvirus 1 and 5 in trigeminal ganglia of beef cattle in Uruguay

Cited by 3 publications

References 28 publications

Bovine Alphaherpesvirus 1, Bovine Alphaherpesvirus 5 and Bubaline Alphaherpesvirus 1 in Palatine Tonsils from Water Buffaloes in Northern Brazil and Possible Links with the Origin of Bovine Alphaherpesvirus Type 5

Bovine Alphaherpesvirus 1, Bovine Alphaherpesvirus 5 and Bubaline Alphaherpesvirus 1 in Palatine Tonsils from Water Buffaloes in Northern Brazil and Possible Links with the Origin of Bovine Alphaherpesvirus Type 5

Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle

Comparison between DNA Detection in Trigeminal Nerve Ganglia and Serology to Detect Cattle Infected with Bovine Herpesviruses Types 1 and 5

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