Detection of bovine papillomavirus type 2 DNA in commercial frozen semen of bulls (Bos taurus)

Silva, M. A. R.; Pontes, Nayara Elis Cabral; Silva, K.M.G. Da; Guerra, Maria Madalena Pessoa; Freitas, Antonio Carlos de

doi:10.1016/j.anireprosci.2011.11.005

Cited by 19 publications

(31 citation statements)

References 41 publications

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“…This is the first study, to the best of our knowledge, which describes the expression of BPV2 in sperm cells of bulls. Our results confirm and better define our previously obtained data on the detection of BPV2 in commercial semen [33]. Also, these results corroborate with our previous study where BPV was demonstrated being expressed in equine semen [34].…”

supporting

confidence: 83%

“…There are few reports focusing on the study of BPV in reproductive tract and its cells [13,35] although the BPV-1 were described in the prepuce and penis [16] and BPV-2 were found infecting placenta and actively expressed in equine semen [30,34]. In an early study, we demonstrate the high incidence of BPV2 in commercial doses of bull semen from national and multinational companies [33]. However, studies are needed to understand the biological significance of BPV presence in semen.…”

mentioning

confidence: 99%

“…The two set of primers were described [28,33]. PCRs were carried out according conditions described [33]. The purified amplicon from PCR were cloned into the pGEMR-TEasy vector (Promega pGEM-T Easy Vector System, Promega, USA), sequenced and analyzed as described above.…”

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confidence: 99%

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Bovine papillomavirus E2 and E5 gene expression in sperm cells of healthy bulls

Silva

Gurgel

et al. 2013

VirusDis.

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Papillomaviruses are found in epithelial lesions and are linked to different carcinogenic processes in humans and other animals. Although BPV has been characterized as epitheliotropic, the presence of viral DNA has been detected in other tissues and fluids, such as fresh semen. The aim of this study was to evaluate the presence and expression of BPV in sperm cells of bulls (Bos taurus) asymptomatic for papillomatosis. A PCR assay was carried out with specific primers to test BPV2 in 26 semen samples. The presence of BPV transcripts was assessed by RT-PCR to E2 and E5 genes. BPV2 DNA was detected in nine out of 26 samples and the expression of E2 and E5 were detected in five out of nine BPV positive samples. This is the first record of BPV2 expression in bull sperm cells.Keywords Bovine papillomavirus Á Semen Á Viral gene expression Papillomaviruses (PVs) are double-stranded DNA tumour viruses identified in a broad range of animal species that belongs to the amniotes, including human being [1]. So far, 13 genotypes of Bovine papillomavirus (BPV1-13) have been characterized [21] and classified in three different genera: Deltapapillomaviruses (BPV1 and 2), Epsilonpapillomaviruses (BPV5 and 8) and Xipapillomaviruses (BPV3, 4, 6, 9, 10 and 11). BPV7 is classified as an unassigned PV genus [12,14]. BPVs from Delta-PVs and BPV5 can infect epithelial and subepithelial fibroblasts inducing fibro-epithelial tumours in cattle, known as fibropapillomas while BPVs from another genus can induce true papillomas [22]. Normally, papillomas regress as a result of cellmediate immune response [17]. However, occasionally papillomas may develop in squamous cell carcinoma as a result of involvement of environmental and genetic cofactors [3,5]. Recently, the co-infection among BPVs in skin lesions (warts) has been described, but the real significance of this finding need to be clarified [7,25,31].PVs are described as epitheliotropic viruses, although their presence has been detected in different tissues and cells [11,20,36]. Few studies have contributed to improved understanding of PV transmission, however, its spread through non-epithelial, however, its spreading of through non-epithelial tissues and fluids has been suggested [6,11].In humans, infected sperm are able to penetrate the oocyte, to deliver HPV genome in the oocyte and HPV genes can be actively transcribed by the fertilized oocyte [10]. There are few reports focusing on the study of BPV in reproductive tract and its cells [13,35] although the BPV-1 were described in the prepuce and penis [16] and BPV-2 were found infecting placenta and actively expressed in equine semen [30,34]. In an early study, we demonstrate the high incidence of BPV2 in commercial doses of bull semen from national and multinational companies [33]. However, studies are needed to understand the biological significance of BPV presence in semen. The present study aimed to evaluate the presence of BPV in sperm cells of healthy bull, as well as the viral gene expression. The semen was collected fro...

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Bovine papillomavirus E2 and E5 gene expression in sperm cells of healthy bulls

Silva

Gurgel

et al. 2013

VirusDis.

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“…Moreover, different BPV types were also identified in the blood of healthy cattle and cattle with papillomalike samples housed together in the same herd. However, some studies have indicated that papillomaviruses can be disseminated to nonepithelial tissues through the bloodstream as a result of infection through semen, milk, urine, or reproductive tissues (7,10,13). It has been suggested that different papillomaviruses may circulate constantly in both symptomatic and asymptomatic cattle housed in the same herd, and these asymptomatic animals could also play a major epidemiological role in the transmission and persistence of the virus.…”

Section: Discussionmentioning

confidence: 99%

“…BPV types 1 and 2 have also been identified in peripheral blood leukocytes (7,8), and in different body fluids, such as milk, colostrum, and semen, obtained from animals with papillomatosis (5,9,10), and even from apparently healthy cattle (8,11) and horses (2,12). BPV-4 is associated with cutaneous papillomatosis, besides upper-gastrointestinal papillomatosis (6,8,10). Furthermore, BPV-9 and BPV-10 have been identified in squamous epithelial papillomas of the udder, whereas BPV-3 and BPV-8 are responsible for cutaneous papillomas (6).…”

Section: Introductionmentioning

confidence: 99%