Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR

Wareth, Gamal; Melzer, Falk; Tomaso, Herbert; Roesler, Uwe; Neubauer, Heinrich

doi:10.1186/s13104-015-1173-1

Cited by 60 publications

(48 citation statements)

References 28 publications

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“…melitensis infection in cattle [97], and small ruminant brucellosis due to B . abortus [96, 98–100]. Therefore, the general tendency of rearing mixed livestock species, and use of communal grazing resources in both the pastoral and mixed crop-livestock systems are indicative of the significance of both B .…”

Section: Resultsmentioning

confidence: 99%

Brucellosis Seropositivity in Animals and Humans in Ethiopia: A Meta-analysis

Tadesse

2016

PLoS Negl Trop Dis

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BackgroundThe objectives of this study were to assess the heterogeneities of estimates and to estimate the seroprevalence of brucellosis in animals and humans in Ethiopia.Methods/Principal findingsData from 70 studies covering 75879 animals and 2223 humans were extracted. Rose Bengal Plate Test (RBPT) and Complement Fixation Test (CFT) in series were the most frequently used serological tests. A random effects model was used to calculate pooled prevalence estimates. The overall True Prevalence of brucellosis seropositivity in goats and sheep were estimated at 5.3% (95%CI = 3.5, 7.5) and 2.7% (95%CI = 1.8, 3.4), respectively, and 2.9% for each of camels and cattle. The prevalence was higher in post-pubertal than in pre-pubertal animals (OR = 3.1, 95% CI = 2.6, 3.7) and in the pastoral than in the mixed crop-livestock production system (OR = 2.8, 95%CI = 2.5, 3.2). The incidence rates of brucellosis in humans of pastoral and sedentary system origins were estimated at 160 and 28 per 100 000 person years, respectively.ConclusionsThe seroprevalence of brucellosis is higher in goats than in other species. Its occurrence is evocative of its importance in the country in general and in the pastoral system in particular. Public awareness creation could reduce the transmission of Brucella spp. from animals to humans and the potential of livestock vaccination as a means of control of brucellosis needs to be assessed.

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Section: Resultsmentioning

confidence: 99%

Brucellosis Seropositivity in Animals and Humans in Ethiopia: A Meta-analysis

Tadesse

2016

PLoS Negl Trop Dis

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“…Os agentes normalmente descritos neste tocante são a Brucella sp. (Samadi et al, 2010;Wareth et al, 2015), Chlamydophila sp. (Pereira et al, 2009;Hazlett et al, 2013), Leptospira sp.…”

Section: Introductionunclassified

Fatores de risco na transmissão e soroprevalência da infecção de Chlamydophila abortus a ovinos e caprinos

Leopoldo

Pinheiro

Alves

et al. 2016

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar os fatores de risco envolvidos na transmissão de Chlamydophila abortus a ovinos e caprinos, bem como verificar a soroprevalência da infecção. Foram selecionadas 110 propriedades, com um total de 500 amostras de ovinos -350 da Microrregião do Alto Médio Gurgueia (MRAMG) e 150 da Microrregião Homogênea de Teresina (MRHT), ambas do Estado do Piauí -e 600 amostras de caprinos -300 da MRAMG e 300 da MRHT. Um questionário epidemiológico foi empregado para identificar os principais fatores de risco. Os anticorpos anti-C. abortus foram detectados pela técnica de fixação de complemento. A prevalência geral da infecção por C. abortus foi de 7,2% (79/1.100). A prevalência em caprinos e ovinos na MRAMG foi de 7,9% (51/650) e, na MRHT, foi de 6,2% (28/450). Nas microrregiões estudadas, a prevalência em ovinos foi de 8,2% (41/500) e, em caprinos, de 6,3% (38/600). O sistema de criação, as práticas reprodutivas e o tipo racial foram fatores de risco relevantes para a infecção em ovinos. Também foram relevantes em caprinos as práticas reprodutivas e a origem das matrizes e dos reprodutores. A raça Dorper apresenta maior suscetibilidade à infecção por C. abortus.Termos para indexação: Capra aegagrus hircus, Ovis aries, clamidofilose, epidemiologia, manejo reprodutivo, pequenos ruminantes. Risk factors in the transmission and seroprevalence of Chlamydophila abortus infection in sheep and goatsAbstract -The objective of this work was to evaluate the risk factors involved in the transmission of Chlamydophila abortus to sheep and goats, as well as to verify the seroprevalence of the infection. One hundred ten farms were selected, with a total of 500 sheep samples -350 from the Microregion of Alto Médio Gurgueia (MRAMG), and 150 from the Homogeneous Microregion of Teresina (MRHT), both in the state of Piauí, Brazil -and 600 goat samples -300 of the MRAMG and 300 of the MRHT. An epidemiological questionnaire was used to identify the main risk factors. Anti-C. abortus antibodies were detected by the complement fixation technique. Overall prevalence of infection by C. abortus was 7.2% (79/1,100). The prevalence in goats and sheep in the MRAMG was 7.9% (51/650), and in the MRHT it was 6.2% (28/450). In the studied microregions, the prevalence in sheep was 8.2% (41/500), and in goats it was 6.3% (38/600). The raising system, reproductive practices, and racial type were significant risk factors for infection in sheep. Also significant in goats were the reproductive practices and the origin of the does and bucks. The Dorper breed shows increased susceptibility to infection with C. abortus.

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“…An additional problem of positive qPCR results in the presence of negative IHC might be explained through vaccination. Most farmers in Jordan vaccinate against brucellosis using live attenuated Rev-1 vaccine, and in previous studies, the vaccine strain was found in the aborted feti (Smirnova et al, 2013;Dougherty et al, 2013;Wareth et al, 2014Wareth et al, , 2015.…”

Section: Discussionmentioning

confidence: 94%

Pathological, Immunohistochemical and Molecular Diagnosis of Abortions in Small Ruminants in Jordan with Reference to Chlamydia abortus and Brucella melitensis

Hailat¹

2018

PVJ

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Abortion in small ruminants is a large problem in many countries in the world including Jordan and cause severe economic losses to sheep and goats producers. Definite diagnosis and identification of the abortive agent and its associated pathological lesions have not been fully described. Therefore, twenty-five formalinfixed and paraffin-embedded sheep and goat placentas taken at the time of abortion were examined for the presence of Chlamydia abortus and Brucella melitensis using gross, histopathological examination, immunohistochemistry and quantitative polymerase reaction (qPCR) for each of these agents. Immunohistochemistry successfully identified the etiologic agents of C. abortus and B. melitensis in 13 and 3 placentas, respectively, with adequate visualization of organisms in expected locations and corresponding lesions. Quantitative PCR for both agents was positive in 14 of the 25 placentas, with 5 being positive for both agents at the same time, and infrequent correlation of IHC with qPCR results. It is proposed that qPCR may be an overly sensitive technique for abortion diagnosis due to chronicity of infection and use of live vaccines and needs to be associated with the pathological lesions.

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Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR

Cited by 60 publications

References 28 publications

Brucellosis Seropositivity in Animals and Humans in Ethiopia: A Meta-analysis

Brucellosis Seropositivity in Animals and Humans in Ethiopia: A Meta-analysis

Fatores de risco na transmissão e soroprevalência da infecção de Chlamydophila abortus a ovinos e caprinos

Pathological, Immunohistochemical and Molecular Diagnosis of Abortions in Small Ruminants in Jordan with Reference to Chlamydia abortus and Brucella melitensis

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