Detection of Brucella Abortus in Caprine and Ovine by Real-Time PCR Assay

Arain, Muhammad Bilawal; Babar, Abdullah; Panhwar, Muhammad Ibrahim; Hal, Khush; Farooq, Muhammad; Lanjar, Zainab; Soomro, Ali Gul; Fazilani, Saqib Ali; Jan, Muhammad Naeem; Lakhani, Loveson; Yousaf, Adnan; Sarki, Inayatullah; Shahnawaz, Rehana; Mathan,

doi:10.11648/j.avs.20210905.13

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“…According to the PCR assay used in this study, all sero-positive samples from cattle, sheep, and goats in serological tests were found to be positive for B. abortus , but B. melitensis was not detected in either ruminant species. This finding is consistent with the findings of [ 30 ], who investigated 1270 goat and 770 sheep sera samples. By real-time PCR, all sero-positive sera samples from these small ruminants were found to be positive for B. abortus , but B. melitensis was not identified.…”

Section: Discussionsupporting

confidence: 93%

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Comparative evaluation of RBPT, I-ELISA, and CFT for the diagnosis of brucellosis and PCR detection of Brucella species from Ethiopian sheep, goats, and cattle sera

Legesse,

Mekuriaw,

Gelaye

et al. 2023

BMC Microbiol

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Background Brucellosis is an economically devastating animal disease and has public health concern. Serological methods such as Rose Bengal Plate Test (RBPT), Complement Fixation Test (CFT), and Indirect-Enzyme-Linked Immunosorbent Assay (I-ELISA) have been used to detect brucellosis. However, there is limited comparative evaluation studies and lack of molecular confirmation of the causative agents in the study areas. The study was aimed to compare RBPT, I-ELISA, CFT, and confirmation using Polymerase Chain Reaction (PCR). A total of 2317 sera samples were collected from brucellosis-affected areas of Ethiopia with no vaccination history. All sera were subjected to comparative serological assays. Post-cross tabulation, sensitivity, and specificity were determined using Receiver Operating Characteristics (ROC) curve analysis software. PCR was performed on 54 seropositive samples using genus- and species-specific primers. Results Among the 2317 sera tested for comparative serological assays, 189 (8.16%) were positive for RBPT, 191 (8.24%) for I-ELISA, and 48 (2.07%) for CFT. Sensitivity to RBPT was 100% (95%) in shoats and 74% (95%) in cattle. Specificity on RBPT was 98.69% (95%), 99.28% (95%), 100% (95%) in sheep, goats, and cattle, respectively. CFT sensitivity was 4 (95%) in sheep, 9.65 (95%) goats, and 72 (95%) cattle. Specificity on CFT was 100% (95%) for sheep, goats, and cattle. A 223bp Brucella genus-specific and 156bp B. abortus species-specific detected. However, B. melitensis not detected. Conclusion In this study, I-ELISA was the most sensitive and specific test. RBPT detected all Brucellosis-infected sheep and goats; nevertheless, it showed false positive in sheep and goats and false negative in cattle. The presence of B. abortus in small and large ruminants was confirmed by PCR. This is the first report of B. abortus detection in small ruminant in Ethiopia. B.abortus detected in non-preferred hosts. The findings suggest further study on molecular epidemiology of Brucella species.

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Section: Discussionsupporting

confidence: 93%

“…B. abortus may be problematic in non-preferred hosts, such as small ruminants. According to the same authors, Brucella’s eco-diversity and multi-pathogenicity allow it to cross the species barrier [ 30 ].…”

Section: Discussionmentioning

confidence: 99%