Detection of Brucella antibodies in selected wild animals and avian species in Pakistan

Ali, Shahzad; Asghar, Muhammad; Imran, Muhammad; Rizwan, Muhammad; Iqbal, Khizar; Qādir, Ghulām; Ahmad, Haroon; Umar, Sajid; Khan, Waseem; Khan, Iahtasham; Neubauer, Heinrich

doi:10.18805/ijar.b-799

Cited by 11 publications

(20 citation statements)

References 15 publications

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“…Data for bucks (3.4%) and for female (0.8%) goats are in contrast to earlier results of [ 29 , 38 ], who found female herds often seropositive. The present results are also opposite to results of a study conducted in Michoacan, Mexico, in small ruminants with 9% prevalence in females and 5% in males [ 39 ].…”

Section: Discussioncontrasting

confidence: 99%

Prevalence and Spatial Distribution of Animal Brucellosis in Central Punjab, Pakistan

Saeed

Ali

Latif

et al. 2020

IJERPH

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Brucellosis is an important zoonotic disease of animals and humans caused by bacteria of the genus Brucella. Brucellae are Gram-negative intracellular bacteria which infect a wide variety of animals including goats, sheep, buffaloes, cows, pigs, and wildlife. The objectives of this study were to determine the seroprevalence and spatial distribution of brucellosis in Central Punjab, Pakistan. A total of 1083 blood samples of goats, sheep, buffaloes, and cows were collected from 38 villages of four districts (Kasur, Faisalabad, Lahore, and Okara) of Punjab, Pakistan, and screened for brucellosis by Rose Bengal Plate test (RBPT) and PCR confirmed. Epidemiological, demographic data and GPS coordinates for every sample were collected. By using interpolation of the Aeronautical Reconnaissance Coverage Geographic Information System (Arc GIS), a surface plot was generated applying inverse distance weight (IDW). It was found that 35 (3.23%) serum samples were positive for brucellosis. In eight (61.5%), six (75%), seven (87.5%), and eight (89%) villages, positive goats, sheep, buffaloes, and cattle were detected, respectively. In general, older animals are more often positive for brucellosis. In goats bucks were more often RBPT positive than females while in sheep, buffaloes, and cattle more females were positive. The spatial distribution of brucellosis shows that it is widely distributed in the western region of the study area in goats and in the South-West region in sheep. Similarly, for buffaloes it is restricted to the south-east and north-west regions, and in cattle brucellosis is present in western region of study area only. Reflected by this study, brucellosis poses a risk for livestock in developing countries due to lack of awareness by officials, owners, and consumers, and control measures are missing. A risk map of brucellosis was generated to develop effective strategies for awareness rising and to improve the quality of control programs in Pakistan.

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Section: Discussioncontrasting

confidence: 99%

Prevalence and Spatial Distribution of Animal Brucellosis in Central Punjab, Pakistan

Saeed

Ali

Latif

et al. 2020

IJERPH

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“…Human brucellosis is well reported and has been described as a professional health hazard [16][17][18]. However, knowledge on the status of infection and possible epidemiological role of non-ruminant domestic animals (equine, canine, and feline) and wildlife in brucellosis remains scarce in the country [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan

et al. 2019

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Brucellosis is an important bacterial zoonosis caused by B. abortus and B. melitensis in Pakistan. The status of canine brucellosis caused by B. canis remains obscure. In total, 181 serum samples were collected from stray and working dogs in two different prefectures viz. Faisalabad (n = 87) and Bahawalpur (n = 94). Presence of antibodies against B. canis and B. abortus/B. melitensis was determined using the slow agglutination test (SAT) and ELISA, respectively. Real-time PCR was performed to detect and differentiate Brucella DNA at the species level. In Faisalabad, the serological prevalence was found to be 9.2% (8/87) and 10.3% (9/87) by SAT and ELISA, respectively. Only one of the ELISA positive samples (1.15%) yielded amplification for B. abortus DNA. In Bahawalpur, 63.8% (60/94) samples were found positive by SAT; however, none of the samples was positive by ELISA or by real-time PCR. Location, age (≥1 year) and body condition (weak) were found to be associated with B. canis infection, whereas presence of wounds was found to be associated with B. abortus infection only. These findings point towards a risk of transmission from dog to livestock and humans and vice versa. The study expects to draw the attention of concerned authorities towards infection prevention and animal welfare. This study warrants further epidemiological investigation on brucellosis in pet dogs and their owners. To the best of our knowledge, this is first ever report on B. canis and B. abortus in dogs in Pakistan.

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“…This cohabitation system enhances potential transmissions from animals. A minimal number of studies [20][21][22][23][24][25] were performed in different countries to investigate whether poultry can harbor the Brucella organism (Table 1). Recently, Ali and colleges collected blood samples from 79 different avian species in Pakistan.…”

Section: Serological Evidence Of Brucellosis In Birdsmentioning

confidence: 99%

“…Recently, Ali and colleges collected blood samples from 79 different avian species in Pakistan. The use of the Rose Bengal test (RBT) showed that two samples from a peafowl (Pavo cristatus) and an Indian blue rock pigeon (Columba livia) were positive [20]. A serological survey of traditionally managed 556 local chickens, 84 guinea fowl, 50 ducks, and 40 turkeys in Borno State, North-Eastern Nigeria using the RBT and the microtiter agglutination test (MAT) showed different degrees of seroprevalence.…”

Section: Serological Evidence Of Brucellosis In Birdsmentioning

confidence: 99%

“…The ubiquitous distribution of brucellae in several reservoirs is the factor determining its global prevalence on all continents and in the majority of countries [19]. In the last decades, anti-Brucella antibodies have been found in different avian species, e.g., chickens, pigeons, and ducks, in some regions of Africa and Asia [20][21][22][23][24][25]. However, the pathogen has not been isolated yet from birds.…”

Section: Introductionmentioning

confidence: 99%

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Susceptibility of Avian Species to Brucella Infection: A Hypothesis-Driven Study

et al. 2020

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Brucellosis is a highly contagious bacterial disease affecting a wide range of animals, as well as humans. The existence of the clinically diagnosed brucellosis in avian species is controversially discussed. In the current study, we set to summarize the current knowledge on the presence of brucellae in avian species. Anti-Brucella antibodies were monitored in different avian species using classical diagnostic tools. Experimental infection of chicken embryos induced the disease and resulted in the development of specific lesions. Few empirical studies have been performed in adult poultry. However, the isolation of brucellae from naturally-infected chickens has not been possible yet.

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Detection of Brucella antibodies in selected wild animals and avian species in Pakistan

Cited by 11 publications

References 15 publications

Prevalence and Spatial Distribution of Animal Brucellosis in Central Punjab, Pakistan

Prevalence and Spatial Distribution of Animal Brucellosis in Central Punjab, Pakistan

Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan

Susceptibility of Avian Species to Brucella Infection: A Hypothesis-Driven Study

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